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Antioxidant effect of Total glycoside of cistanche salsa on mouse tissues

Wang Xiaowen, Li Linlin, Mu Hu Yati, Wang Xuefei, Du Niansheng

【Abstract】 Objective: To study the antioxidant effect of Total glycoside of cistanche salsa (GCS)on mouse tissues. Methods: Pyrogallol autoxidation, thiobarbituric acid (T BA), and fluorescence methods were used to determine SOD activity, M DA, and lipofuscin content in mouse heart, liver, brain, and kidney tissues, respectively. Results: The GCS (Total glycoside of cistanche salsa) (125mg/kg, 250mg/kg) group can significantly increase the SOD activity in the brain and kidney tissues, and at 250 mg/kg it can increase the SOD activity in the heart and liver tissues, and significantly reduce the activity of SOD in the heart and liver tissues. Tissue M DA and lipofuscin content. Conclusion: The results suggest that GCS (Total glycoside of cistanche salsa) can effectively improve the antioxidant capacity of body tissues and prevent tissue lipid peroxidation damage.

Keywords: Total glycoside, cistanche salsa, SOD, malondialdehyde, lipofuscin, antioxidant

Cistanche salsa (C. A. Mey.) G. Beck has the effects of nourishing the middle and nourishing qi, nourishing essence, and aphrodisiac. The probability of appearing in ancient anti-aging and prolonging anti-aging prescriptions is second only to ginseng. In modern pharmacological research, cistanche salsa can enhance the immune function of the body [1], promote memory, improve intelligence [2], and anti-aging [3]. The crude preparation of cistanche salsa significantly reduces the content of myocardial lipofuscin [3]. This article mainly studies the effect of Total glycoside of cistanche salsa on the antioxidant effect and lipofuscin content of mouse heart, liver, brain, and kidney tissues.

total glycoside of cistanche salsa has an antioxidant effect

1 Material

1.1 Medicinal materials and reagents:

Total glycoside of cistanche salsa was extracted and identified by the Plant Chemistry Laboratory, Department of Pharmacy, Xinjiang Medical University [4]. Pyrogallol, produced by the second chemical plant in Zunyi City; thiobarbituric acid, produced by the second Shanghai reagent plant; quinine, produced by the second Shanghai reagent plant; 1, 1, 3, 3-tetra ethoxy alkane (T EP ), Coomassie Brilliant Blue are all products of Sigma.

1.2 Animals:

NIH mice, provided by the Animal Experiment Center of Xinjiang Institute of Endemic Diseases.

cistanche is a tyrosinase inhibitor

2 Methods and results

2.1 Effect of GCS (Total glycoside of cistanche salsa) on SOD activity and MDA content in mouse tissue

Divided into normal saline control group (NS) and different doses of GCS (Total glycoside of cistanche salsa) group (62.5, 125, 250mg kg-1 10 IH juvenile mice, weighing (15.2 ± 1.5) g, ig the same volume of NS, once a day for 30 consecutive days, each group was sacrificed 1.5 hours after the last dose, the cervical vertebrae were sacrificed, and the chest and abdomen were opened. Immediately take out the heart, liver, brain, and kidney tissues, rinse with frozen NS, weigh the tissues, grind, prepare tissue homogenate, centrifuge at 3000 r·min-1 for 30 min, take the supernatant for testing, and use pyrogallol for auto-oxidation The SOD activity of the tissue was measured by the method [5]; the MDA content of the tissue was measured by the TBA color method [6]; the tissue protein was measured by the Coomassie brilliant blue method [7].

The results showed that the SOD activity in the heart and liver tissues of the 5-month-old NS group was significantly lower than that of the 1-month-old NS group, and the MDA content in each tissue was significantly higher than that of the 1-month-old NS group, the GCS (Total glycoside of cistanche salsa) group, and the 5-month-old NS group. Compared with the age NS group, the SOD activity in the heart and liver tissues was significantly increased at 250 mg·kg -1, which was close to the 1-month-old group; the 125 and 250 mg·kg -1 groups all decreased the MDA content in the heart and liver tissues, which was close to the 1-month-old group The 125 and 250 mg·kg -1 groups significantly increased the SOD activity in the brain and kidney tissues, and significantly reduced the MDA content, which was close to the 1-month-old group (Table 1).

Table 1 Effects of Total Glycosides of Cistanche Salsa on SOD Activity and MDA Content in Heart, Liver, Brain, and Kidney Tissues of Mice

2.2 The effect of GCS (Total glycoside of cistanche salsa) on lipofuscin content in mouse tissues.

Select 40 healthy 5-month-old NIH mice, weighing (32 ± 1.5) g, both male and female, and divided into 4 groups according to their weight balance. , NS control group and different dose GCS (Total glycoside of cistanche salsa) group (62.5, 125, 250mg·kg -1·d-1, ig), continuous 30 days, and 10 NIH mice of 1-month-old, Bodyweight (15 ± 1.5) g, ig the same volume of NS, once a day, for 30 days, each group was sacrificed 1.5h after the last dose of cervical vertebrae, and the heart, liver, brain, and kidney tissues were taken respectively. Filter paper to absorb water, weigh 0.2g of each tissue, according to Sohal [8] method, divide the tissue into 8ml homogenate with chloroform-methanol (2∶1) mixture, and incubate it in a 40 ℃ water bath for 5 min, 3000r ·Min -1 Centrifuge for 10 minutes, take the chloroform layer on Shimadzu RF-540 fluorescence spectrophotometer, excitation wavelength 351.6nm, emission wavelength 451.1nm, measure the fluorescence intensity, according to the fluorescence intensity of quinine sulfate 1μg·ml -1 As 100 units, calculate the fluorescence counting unit per gram of wet tissue, expressed in U·g -1.

The results are shown in Table 2. Each group is compared with the 5-month-old mouse NS group. In the 1-month-old NS group, the lipofuscin content in liver and brain tissue is significantly lower than that in the 5-month-old NS group. GCS (Total glycoside of cistanche salsa) The 250 mg·kg -1 group significantly reduced the lipofuscin content in the heart tissue, 125 and 250 mg·kg -1 both significantly reduced the lipofuscin content in the liver and kidney tissues, and the decrease was more significant at 250 mg·kg -1.

Table 2 Effects of Total glycoside of cistanche salsa on the lipofuscin content of heart, liver, brain and kidney tissues in mice

3 Discussion

The free radical theory of aging believes that with age, the generation and elimination of free radicals in the body is out of balance, which reduces the activity of free radical scavenging enzyme SOD, causes lipid peroxidation in biological membranes, and destroys the structure and function of biological macromolecules. Damage to the heart, liver, brain, kidney, etc., the main organs in the body. In addition, lipofuscin is a product of free radical oxidation, and its content in tissues is positively correlated with lipid peroxidation, which is an indicator of aging. Therefore, maintaining the homeostasis and balance of free radicals in the body, eliminating harmful free radical reactions, interrupting lipid peroxidation, and reducing lipid peroxidation products are very important for delaying aging. This depends on improving the activity of the free radical scavenging enzyme system. Current research suggests that the anti-aging mechanism of most traditional Chinese medicines may be related to their antioxidant effects [9]. This study found that the active ingredient Total glycoside in cistanche salsa can significantly increase the activity of free radical scavenging enzyme SOD in the heart, liver, brain, and kidney tissues of 5-month-old mice, and is sensitive to the effects of brain and kidney tissues. And reduce the content of MDA and lipofuscin in the above-mentioned tissues. It is suggested that GCS (Total glycoside of cistanche salsa) has anti-oxidant and anti-aging effects.

total glycoside of cistanche salsa

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