Dietary Arsenic Supplementation Induces Oxidative Stress By Suppressing Nuclear Factor Erythroid 2-related Factor 2 in The Livers And Kidneys Of Laying Hens

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ABSTRACT

This study investigated the effects of dietary arsenic supplementation on laying performance, egg quality, hepatic and renal histopathology, and oxidative stress in the livers and kidneys of laying hens. Furthermore, the nuclear factor erythroid 2-related factor 2 (Nrf2)-Kelch-like ECH-associated protein 1(Keap1) pathway was explored to reveal the molecular mechanism of the stress. Five hundred and twelve 40-week-old Hyline White laying hens were randomly allocated to 4 groups with 8 pens per group and 16 hens per pen. The doses of arsenic administered to the 4 groups were 0.95, 20.78, 40.67, and 60.25 mg/kg. The results revealed that dietary arsenic supplementation significantly reduced hen-day egg production (P, 0.05), average egg weight (P, 0.05), Haugh units (P, 0.05), albumen height (P, 0.05), and eggshell strength (P, 0.05). Dietary arsenic supplementation also induced the accumulation of arsenic and histopathological damages in the liver and kidney. In accordance, dietary arsenic supplementation signifificantly enhanced serum alanine aminotransferase (P, 0.05), aspartate aminotransferase (P, 0.05), blood urea nitrogen (P, 0.05), and uric acid (P, 0.05) levels. After arsenic exposure, the activities of superoxide dismutase (SOD) (P , 0.05), catalase (P , 0.01), glutathione reductase (P, 0.05), glutathione peroxidase (P, 0.05), and glutathione content (P, 0.05) were signifificantly decreased, while the malondialdehyde level was significantly increased (P, 0.05) in the liver and kidney. Positive correlations occurred between antioxidant enzyme activities and antioxidant enzyme gene expression in the liver and kidney, except for renal manganese superoxide dismutase gene expression and SOD activity. Additionally, hepatic and renal Nrf2 mRNA expression was positively correlated with antioxidant gene expression and negatively correlated with Keap1 mRNA expression. In summary, dietary arsenic supplementation induced oxidative stress by suppressing the Nrf2-Keap1 pathway in the livers and kidneys of laying hens.

Keywords: arsenic, laying hen, Nrf2-Keap1 pathway, oxidative stress, kidneys

INTRODUCTION

In recent years, environmental hazards have been found to occur in increasing concentrations. Arsenic is a highly metalloid toxicant, even at a very low concentration in poultry feedstuff. Its physiological role in poultry is well defined, as it is necessary for the synthesis of methionine metabolites including cysteine. The recommended concentrations of arsenic in poultry feedstuff are between 0.012 and 0.050 mg/kg (Balo
s et al., 2019). However, a previous investigation showed that the arsenic concentrations in poultry feedstuff are likely beyond the tolerance levels of animals when the poultry feedstuff contains seaweeds, cupric carbonate, cupric sulfate pentahydrate, dicopper chloride trihydroxide, or ferrous carbonate (Adamse et al., 2017). Kazi et al. (2013) reported that there is a high possibility that arsenic in poultry feedstuff affects the health of broiler chickens. The excessive amounts of arsenic in poultry feedstuff and its toxicological effects on poultry are still serious problems. When excess arsenic enters an animal, it can induce a variety of adverse health effects, such as immunotoxicity, respiratory toxicity, cardiovascular toxicity, hepatotoxicity, hepatotoxicity, nephrotoxicity, neurovirulence, reproductive toxicity, and genotoxicity. The toxicological effects of arsenic on visceral organs have been primarily documented in mammalian studies, suggesting that arsenic poses a risk to hepatic and renal functions (Waalkes et al., 2004; Mazumder, 2005; Zheng et al., 2014). Nevertheless, the toxicological effects of dietary arsenic exposure on the liver and kidney of laying hens are still unclear. The toxicity of arsenic in animals is closely related to oxidative stress, which disturbs the pro/antioxidant balance (Flora, 2011). When arsenic enters a cell, it binds with intracellular glutathione (GSH) or oxidizes it, leading to free radical generation. As we know, cytoprotective genes can be regulated by a number of intracellular transcription factors, including nuclear factor erythroid 2-related factor 2 (Nrf2), activator protein 1, and nuclear factor kappa-B (Kwak et al., 2001). The transcription factor Nrf2 is a vital molecule that regulates stress levels in cells. Under quiescent conditions, Nrf2 interacts with Kelch-like ECH-associated protein 1 (Keap1), which is mostly located in the cytoplasm. When oxidative stress is triggered, Nrf2 translocates from the cytoplasm to the nucleus after separating from the Keap1 molecule and then activates the expressions of cytoprotective genes (Motohashi and Yamamoto, 2004). Thereafter, cytoprotective genes further regulate the activities of downstream antioxidant enzymes, including superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GSH-Px), and catalase (CAT). A previous study has shown that the transcription factor Nrf2 participates in arsenic-induced stress in mammals (Sinha et al., 2013). However, the exact effects of arsenic exposure on oxidative stress effects in laying hens remain elusive. In the present study, we investigated the effect of dietary arsenic supplementation on laying performance, egg quality, serum biochemical indices, hepatic and renal histopathological changes, and oxidative stress in laying hens. Furthermore, the Nrf2-Keap1 pathway was explored to identify the molecular mechanism in the livers and kidneys of laying hens. This study provides some insights on the biological theory of dietary excess arsenic toxicity in the liver and kidney of laying hens.

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MATERIALS AND METHODS

This study was approved by the Institutional Animal Care and Use Committee. All experimental procedures performed on animals were implemented in accordance with the Chinese Association for Laboratory Animal Sciences.

Animals, Diets, and Experimental Design

Five hundred and twelve 40-week-old Hyline White laying hens with similar body conditions were randomly selected and separated into 4 groups. Each group contained 8 replicates of 16 birds. Arsenic was added to the basal corn-bean diet at 4 different concentrations (0, 20, 40, and 60 mg/kg; in the form of arsanilic acid) (Supplement Table 1). The concentrations of arsenic in the feed were measured by hydride generation atomic absorption spectrometry according to previous methodology (Dos Passos et al., 2012). The actual concentrations of arsenic in the 4 groups were 0.95, 20.78, 40.67, and 60.25 mg/kg. The birds were kept in cages (60 ! 50 ! 50 cm3) equipped with 1 feeder and 2 nipple drinkers, and 2 hens were housed per cage. Throughout the entire experimental period, the hens had free access to feedstuff and drink. The entire experiment lasted 10 wk, including a 1-week adjustment period and a 9-week formal experimental period.

Laying Performance and Egg Quality

Throughout the entire experimental period, laying performance indices were recorded daily, including feed consumption, hen-day egg production, and egg weight (EW). Determinations of feed intake and EW in each group were performed using a sensitive weight scale (XS2002S, Mettler Toledo, Zurich, Switzerland). Feed conversion ratio (FCR) was calculated according to the following formula: FCR 5 feed intake in grams/ egg mass in grams. A total of 40 eggs from each group were randomly collected to measure egg quality parameters within 24 h of oviposition at the end of the experiment. The eggs were weighed using a sensitive weight scale (XS2002S, Mettler Toledo). Thereafter, Haugh unit, albumen height, yolk color, and eggshell strength were determined by a digital egg tester (DET6000, Nabel Co. Ltd., Kyoto, Japan). Eggshell thickness with the inner membrane was determined at the sharp, middle, and blunt region of the egg using a dial gauge micrometer(547-350, Mitutoyo, Kawasaki, Japan), and the mean values were used for the statistical analysis.

Collection of Samples

After the rearing experiment, 32 birds from each group were randomly selected and euthanized by cutting off the neck veins. Blood samples were collected in sterile centrifuge tubes and immediately transported to the laboratory for measurement of serum biochemical indices. Thereafter, the birds were dissected, and the liver and kidneys were removed from the abdominal cavity. The liver and kidney samples were cut into 4 parts. One part was immediately fixed in 4% paraformaldehyde for histopathological examination. The other 3 parts were immediately stored in liquid nitrogen for further determination of oxidative stress parameters, arsenic deposition, and gene expression.

Arsenic Deposition Assay

After measurement of egg quality, the yolk was separated from the albumen. Accumulation of arsenic in the albumen and yolk was measured by hydride generation atomic absorption spectrometry according to previous methodology (Dos Passos et al., 2012). Accumulation of arsenic in the whole egg was calculated by summing the arsenic content in the albumen and yolk. In a similar way, hydride generation atomic absorption spectrometry was used to determine the accumulation of arsenic in the liver and kidney of laying hens (Dos Passos et al., 2012).

Determination of Serum Biochemical Indices

Total protein, albumin, globulin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels are important indexes for evaluating liver function. These parameters were measured using appropriate assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) in accordance with the manufacturer's instructions. Blood urea nitrogen (BUN), uric acid (UA), and creatinine (CT) levels are important indexes for evaluating kidney function and were determined using detection kits (Nanjing Jiancheng Bioengineering Institute).

Histopathological Changes

Hepatic and renal tissues fixed in 4% paraformaldehyde were dehydrated in 70, 80, 90, 95, and 100% ethanol and ultimately embedded in paraffin. The tissues were sliced into 6-mm thickness sections and then stained with hematoxylin and eosin. Thereafter, observations of histopathological changes in the liver and kidney tissues were performed by a pathologist under an optical microscope (Olympus, Melville, NY).

Lipid Peroxidation (LPO) and Antioxidant Enzyme Activity Assays

The activities of SOD, CAT, GR, and GSH-Px, and the contents of malondialdehyde (MDA) and GSH in the liver and kidney were determined by appropriate analysis kits (Nanjing Jiancheng Bioengineering Institute). Briefly, MDA content was measured by a spectrophotometric method based on the reaction between thiobarbituric acid and MDA (Janero, 1990). GR activity and GSH content were determined by using 5,5- dithiobis(2-nitrobenzoic acid) (Carlberg and Mannervik, 1985; Abegg et al., 2012). SOD activity was determined according to the inhibitory reaction between nitro blue tetrazolium reduction and xanthine oxidase. CAT activity was determined based on the formation of a stable hydrogen peroxideammonium molybdate complex (Aebi, 1984). The GSH-Px activity was determined by evaluating the reduction of t-butyl hydroperoxide (Wheeler et al., 1990).

Total RNA Isolation and Real-Time Quantitative PCR

Total RNA was isolated from the liver and kidney tissues with the Trizol RNAiso Kit (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions. The RNA samples were reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (TaKaRa, Dalian, China). The forward and reverse primers for manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), CAT, GR, GSH-Px, Nrf2, Keap1, and the housekeeping gene (b-actin) are shown in Supplemental Table 2. Gene abundance was measured by a StepOnePlus Real-Time PCR system (ABI 7500, Applied Biosystems, Foster City, CA). The cycling conditions were 95
C for 30 s followed by 35 cycles of 95
C for 5 s, 59
C for 10 s, and 72
C for 30 s. The fold difference in mRNA expression was measured using the relative quantification method utilizing real-time PCR efficiencies and normalized to the level of b-actin, to compare the relative CT changes among all groups (Livak and Schmittgen, 2001).

Statistical Analyses

All data are expressed as the mean 6 SE. Statistical analysis was performed by one-way ANOVA using SPSS version 20.0 (SPSS Inc., Chicago, IL). When the differences between the groups were significant (indicated by P, 0.05), the means were compared with Tukey’s honestly significant difference for post hoc multiple comparisons. Pearson correlations were analyzed by bivariate correlation analysis (SPSS version 20.0, SPSS Inc.). The significance and correlation coefficients are represented as “p” and “r,” respectively.

RESULTS

Laying Performance and Egg Quality Compared with those in the 0.95 mg/kg arsenic group, hen-day egg production, and EW were signifificantly decreased in the 60.25 mg/kg arsenic group (P, 0.05). However, dietary arsenic did not affect feed intake or FCR (Table 1). Compared with that in the 0.95 mg/kg arsenic group, the Haugh unit was signifificantly decreased in the 40.67 mg/kg (P, 0.05) and 60.25 mg/kg (P, 0.05) arsenic groups. Furthermore, both albumen height and eggshell strength were signifificantly decreased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05), plateaued in the 40.67 mg/kg arsenic group, and sharply decreased in the 60.25 mg/kg arsenic group (P, 0.05). Dietary arsenic did not affect yolk color or eggshell thickness (Table 1)

Deposition of Arsenic

The deposition of arsenic in the albumen (P, 0.05), yolk (P, 0.05), and the whole egg (P, 0.05) significantly increased as the dose of dietary arsenic increased from 0.95 to 60.25 mg/kg (Table 2). Similarly, as the dose of dietary arsenic increased from 0.95 to 60.25 mg/kg, the deposition of arsenic in the liver (P, 0.05) and kidney (P, 0.05) signifificantly increased (Table 2)

Correlation Analysis Between Egg Quality and Deposition of Arsenic in the Egg

The deposition of arsenic in the albumen was negatively correlated with the Haugh unit (r 5 20.622, P, 0.01), albumen height (r 5 20.878, P, 0.01), and eggshell strength (r 5 20.897, P, 0.01). Meanwhile, the deposition of arsenic in the yolk was also negatively related to the Haugh unit (r 5 20.654, P, 0.01), albumen height (r 5 20.893, P, 0.01), and eggshell strength (r 5 20.902, P, 0.01). Similarly, negative relationships were found between the deposition of arsenic in the whole egg and the Haugh unit (r 5 20.640, P, 0.01), albumen height (r 5 20.888, P, 0.01), and eggshell strength (r 5 20.902, P, 0.01) (Table 3)

Serum Biochemical Indices

Compared with those in the 0.95 mg/kg arsenic group, ALT levels were signifificantly increased in the 20.78 mg/ kg (P , 0.05), 40.67 mg/kg (P , 0.05), and 60.25 mg/kg (P , 0.05) arsenic groups. Meanwhile, AST levels in the 60.25 mg/kg arsenic group were signifificantly increased compared with those in the 0.95 mg/kg arsenic group (P, 0.05). Dietary arsenic did not affect total protein or albumin levels in serum (Table 4).

Compared with those in the 0.95 mg/kg arsenic group, both BUN and UA levels were signifificantly increased in the 60.25 mg/kg arsenic group (P, 0.05), whereas dietary arsenic exposure did not affect the CT level in the serum (Table 4).

Histopathological Changes

The appearance of hepatic tissue was normal and unaltered in the 0.95 mg/kg arsenic group. However, as the dose of dietary arsenic increased from 20.78 to 60.25 mg/ kg, the proliferation of the bile duct, hepatocyte steatosis, and deformation of the central vein became more severe (Figures 1A–1D). The appearance of renal tissue was normal and unaltered in the 0.95 mg/kg arsenic group. However, there was severe glomerular shrinkage in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group. As the dose of dietary arsenic increased from 40.67 to 60.25 mg/ kg, enlargement of renal tubules, tubular fifibrosis, and hyalinization became more severe (Figures 1E–1H).

Oxidative Stress Biomarkers

Compared with those in the 0.95 mg/kg arsenic group,hepatic MDA levels were signifificantly increased in the 60.25 mg/kg arsenic group (P , 0.05), and renal MDA levels were significantly increased in the 20.78 mg/kg (P , 0.05), 40.67 mg/kg (P , 0.05), and 60.25 mg/kg (P , 0.05) arsenic groups (Figure 2A). GSH levels in the liver and kidney signifificantly decreased in the 20.78 mg/kg arsenic group compared with those in the 0.95 mg/kg arsenic group (P, 0.05), and plateaued in the 40.67 and 60.25 mg/kg arsenic groups (Figure 2B). Hepatic SOD activity was signifificantly decreased in the 40.67 mg/kg (P, 0.05) and 60.25 mg/kg (P, 0.05) arsenic groups compared with the 0.95 mg/kg arsenic group. Renal SOD activity was signifificantly decreased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/kg arsenic groups (Figure 2C). CAT activity in the liver and kidney signifificantly decreased as the dose of dietary arsenic increased from 0.95 to 60.25 mg/kg (P, 0.05, Figure 2D). Compared with that in the 0.95 mg/kg arsenic group, GR activity in the liver and kidney was signifificantly decreased in the 60.25 mg/kg arsenic group (P, 0.05, Figure 2E). In addition, compared with the 0.95 mg/kg arsenic group, hepatic GSH-Px activity was signifificantly decreased in the 60.25 mg/kg arsenic group (P, 0.05), and renal GSHPx activity sharply decreased in the 20.78 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/kg arsenic groups (Figure 2F).

Gene Expressions of Antioxidant Enzymes, Nrf2, and Keap1 Molecules

Hepatic CuZnSOD gene expression was signifificantly decreased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/kg arsenic groups. Renal CuZnSOD gene expression was signifificantly decreased in the 40.67 mg/kg (P , 0.05) and 60.25 mg/kg (P , 0.05) arsenic groups compared with the 0.95 mg/kg arsenic group (Figure 3A). Compared with the 0.95 mg/kg arsenic group, hepatic MnSOD gene expression was signifificantly decreased in the 20.78 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/kg arsenic groups. Renal MnSOD gene expression was not signifificantly different among the groups (Figure 3B). Hepatic CAT gene expression was signifificantly decreased in the 20.78 mg/ kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/kg arsenic groups. Renal CAT gene expression was signifificantly decreased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 mg/kg arsenic group, and was sharply decreased in the 60.25 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05, Figure 3C). GR gene expression in the liver and kidney was signifificantly decreased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/kg arsenic groups (Figure 3D). In addition, hepatic GSH-Px gene expression significantly decreased as the dose of dietary arsenic increased from 0.95 to 40.67 mg/kg (P, 0.05) and then plateaued in the 60.25 mg/kg arsenic group. Renal GSH-Px gene expression signifificantly decreased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/kg arsenic groups (Figure 3E). Nrf2 gene expression in the liver and kidney was significantly decreased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/ kg arsenic groups (Figures 4A and 4C). In contrast, Keap1 gene expression in the liver and kidney was sharply increased in the 20.78 mg/kg arsenic group compared with the 0.95 mg/kg arsenic group (P, 0.05) and plateaued in the 40.67 and 60.25 mg/ kg arsenic groups (Figures 4B and 4D).

Correlation Analyses Related to the Nrf2- Keap1 Pathway

The gene expression of CuZnSOD (liver, r 5 0.613, P , 0.01; kidney, r 5 0.687, P , 0.01), CAT (liver, r 5 0.738, P , 0.01; kidney, r 5 0.903, P , 0.01), GR (liver, r 5 0.477, P , 0.05; kidney, r 5 0.485, P , 0.05), and GSH-Px (liver, r 5 0.450, P , 0.05; kidney, r 5 0.767, P , 0.01) in the liver and kidney, and hepatic MnSOD gene expression (r 5 0.707, P , 0.01) were positively correlated with the activities of their corresponding antioxidant enzymes. Furthermore, Nrf2 gene expression was positively correlated with the gene expressions of CuZnSOD (liver, r 5 0.756, P , 0.01; kidney, r 5 0.736, P , 0.01), CAT (liver, r 5 0.893, P , 0.01; kidney, r 5 0.740, P , 0.01), GR (liver, r 5 0.837, P , 0.01; kidney, r 5 0.915, P , 0.01), and GSH-Px (liver, r 5 0.822, P , 0.01; kidney, r 5 0.722, P , 0.01) in the liver and kidney, and hepatic MnSOD gene expression (r 5 0.720, P , 0.01). There was a negative correlation between Nrf2 and Keap1 mRNA expression (liver, r 5 20.746, P , 0.01; kidney, r 5 20.771, P , 0.01) in the liver and kidney. In addition, there was no correlation between MnSOD gene expression and SOD enzymatic activity, or Nrf2 mRNA expression in the kidneys of laying hens (Table 5).

DISCUSSION

Arsenic is a ubiquitous and toxic metalloid in nature. It induces several toxicoses in humans and animals, including hepatotoxicity, nephrotoxicity, neurovirulence, immunotoxicity, cardiovascular toxicity, hepatotoxicity, and reproductive toxicity (Mandal and Suzuki,2002). Arsenic most robustly targets the reproductive system of animals. Previous studies have shown that dietary roxarsone exposure disturbs laying rate and egg production (Chiou et al., 1999; Zhang et al., 2017). In this study, dietary arsenic supplementation signifificantly decreased laying performance, including egg production and EW. Previous studies have found that dietary arsenic supplementation induces the accumulation of arsenic in eggs and reduces egg quality (Chiou et al., 1998; Zhang et al., 2017). In the present study, dietary arsenic supplementation signifificantly decreased the Haugh unit, albumen height, and eggshell strength. Except for yolk color and eggshell thickness, negative correlations were found between the deposition of arsenic in eggs and egg quality parameters. This suggests that Haugh unit, albumen height, and eggshell strength might be affected by the deposition of arsenic in the egg. As we know, the palisade layer thickness in the eggshell is the determinant of an eggshell thickness (Ruiz and Lunam, 2000), while pigment deposition determines yolk color. Thus, we speculated that dietary arsenic supplementation might not affect the thickness of the palisade layer of pigment deposition in the eggs of laying hens. Emerging evidence indicates that hepatic and renal disorders are common in mammals after arsenic exposure (Liu and Waalkes, 2008; Huang et al., 2009). A previous study showed that arsenic exposure induces histopathological lesions in the liver, including tissue disorientation, peliosis, and vacuolization accompanied by karyolysis, apoptosis, and necrosis of hepatocytes in Channa punctatus (Roy and Bhattacharya, 2006). In this study, we observed severe changes in the proliferation of the bile duct, hepatocyte steatosis, and deformation of the central vein in the liver as the dose of dietary arsenic increased from 20.78 to 60.25 mg/kg. Roy and Bhattacharya (2006) also found that arsenic exposure induces shrinkage of the glomerulus, irregularities in the renal tubule, and an increase in Bowman’s space. In the present investigation, as the dose of dietary arsenic

increased from 20.78 to 60.25 mg/kg, renal histopathological changes were very severe, including enlargement of the renal tubules, glomerular shrinkage, and tubular fifibrosis and hyalinization, which is consistent with a previous study (Roy and Bhattacharya, 2006). According to previous reports, serum AST and ALT levels have been proven to be surrogate markers of the hepatic inflammatory reaction and fifibrosis (Wang et al., 2008; Khattab et al., 2015). In this study, increases in AST and ALT levels in serum implied that the hepatic inflflammatory response was intensified after arsenic exposure, which was consistent with the observed histopathological changes in the livers of laying hens. Patel and Kalia (2013) also found that arsenic-induced hepatotoxicity is manifested by an increase in serum ALT and AST levels in Wistar rats. Renal function is routinely monitored by BUN, CT, and UA levels in the serum. We found that serum BUN and UA levels were signifificantly increased, and the serum CT level tended to increase after dietary arsenic supplementation, implying that kidney damages resulted from arsenic exposure in laying hens, which is consistent with previous research (Liu et al., 2000). It is well established that tissue damage induced by arsenic exposure is closely related to oxidative stress (Jomova et al., 2011). When oxidative stress is triggered, intracellular reactive oxygen species (ROS) induce LPO, which can be monitored by intracellular MDA levels (Storey, 1996). In this study, hepatic and renal MDA levels were signifificantly increased after dietary arsenic supplementation, implying that there was an increase in LPO, which may have indicated oxidative injury in the livers and kidneys of laying hens. GSH also plays a vital role in the regulation of intracellular oxidative stress (Finkel and Holbrook, 2000). Compared with those in the 0.95 mg/kg arsenic group, GSH levels

were signifificantly decreased in the groups treated with higher concentrations of arsenic, which suggests that arsenic might bind with GSH to attenuate the antioxidant capabilities of the liver and kidney. Flora et al. (1997) reported that arsenic exposure reduces the GSH concentration and produces pronounced lesions in the livers and kidneys of rats. In addition, intracellular antioxidant enzymatic systems confer protective roles to protect against oxidative stress, including SOD, CAT, GR, and GSH-Px (Finkel and Holbrook, 2000). In this study, dietary arsenic supplementation signifificantly reduced the activities of SOD, CAT, GR, and GSH-Px in the livers and kidneys of laying hens. When antioxidant systems cannot neutralize the excess of intracellular ROS, oxidative damage occurs due to LPO, which might, in turn, attenuate the activities of antioxidant enzymes. A previous study similarly reported that arsenic induces oxidative stress in the rat kidney (Sener et al., 2016). Antioxidant enzymes are proteins and might be regulated by genes at the transcriptional level. In the present study, arsenic exposure signifificantly decreased the mRNA expression of CuZnSOD, CAT, GR, and GSHPx. Furthermore, the gene expression of CuZnSOD, CAT, GR, and GSH-Px was positively correlated with the activities of antioxidant enzymes, implying that arsenic exposure reduced the activities of antioxidant enzymes by inhibiting mRNA expression. Similarly reported correlations between the activities of antioxidant enzymes and gene expression in the livers and kidneys of laying hens after mercury exposure. However, dietary arsenic supplementation did not affect MnSOD expression in the kidney. This may have been because SOD has several isoenzymes, and its activity is not affected by the MnSOD gene. Nrf2 plays a vital role in the defense system against oxidative stress. Under basal conditions, Nrf2 binds to Keap1 in the cytoplasm. Once the intracellular ROS level is high enough to modify the reactive thiol groups of Keap1, Nrf2 much more easily translocates into the nucleus, where it irritates the antioxidant-responsive

element and then activates downstream protective genes (Sinha et al., 2013). In this study, we found that arsenic exposure signifificantly reduced Nrf2 gene expression and the expression of downstream antioxidant enzymes. Downregulation of Nrf2 and downstream antioxidant enzyme genes after arsenic exposure suggested that arsenic inhibited the expression of antioxidant enzyme genes by suppressing Nrf2 gene expression in the liver and kidney. In addition, the enhancement of Keap1gene expression was negatively correlated with Nrf2 and antioxidant enzyme gene expression, implying that upregulation of cytoplasmic Keap1 promotes Nrf2 translocation from the cytoplasm to the nucleus (Kensler et al., 2007). A similar study reported that the intracellular Nrf2-Keap1 pathway is inactivated in response to arsenic exposure (Janasik et al., 2018). Nevertheless, a previous study also reported that arsenic exposure enhances the Nrf2-Keap1 pathway to protect against oxidative damage (Massrieh et al., 2006). These findings are not inconsistent with this study. In the early stages of oxidative stress, the protective effects of Nrf2-Keap1 might be activated to prevent oxidative stress. Nonetheless, the Nrf2-Keap1 pathway might not resist oxidative damages induced by sustained exposure to a high dose of arsenic (Kensler et al., 2007). Thereafter, the Nrf2- Keap1 pathway might be inhibited, and intracellular oxidative damage or even apoptosis may occur in the livers and kidneys of laying hens. In view of the present results, this study provides some new evidence for hepatic and renal antioxidant defense under arsenic exposure in laying hens and elucidates a central role of the Nrf2-Keap1 pathway in arsenic-induced oxidative stress for the fifirst time. In summary, dietary arsenic supplementation reduced the laying performance and egg quality of laying hens. Histopathological damages occurred in the liver and kidney after dietary arsenic exposure. In addition, dietary arsenic exposure induced hepatic and renal oxidative stress by impairing the Nrf2-Keap1 pathway in laying hens.