Essential Oils As A Potential Neuroprotective Remedy For Age-Related Neurodegenerative Diseases Part 2

Please contact oscar.xiao@wecistanche.com for more information

4. Discussion

EOs contain the essence of different scents and the properties of their originating plants. These volatile oils display various biological activities [154]. They are mainly used in the beverage, fruit, cosmetic, and fragrance industries [155]. EOs derived from the steam distillation process are mainly used in pharmacological activities and food products, while extracts from lipophilic solvents are utilized in the fragrance industry [156]. Several EOs have been well-known for their usage in fragrances and flavors for hundreds of years. usage in the fragrance industry is mainly due to their attractive odor. Cistanche Extract Anti Radiation The extensive benefits offered by EOs signify the continuous demand that is seen to be increasing steadily.

4.1.The Source of EOs

As mentioned before, EOs can be extracted and obtained from various parts of plants. Clove's EO is derived from the Syzygium aromaticum tree's aromatic flower buds with origin from Maluku, Indonesia contains the powerful scent used in spiced foods [154,155]. Eucalyptus globulus oil is mint-like, with properties such as a decongestant, pain reliever, antimicrobial agent, immunostimulant, flu, and cold/cough treatment, as well as for mental clarity in aromatherapy[157,158]. One of the most influential EOis from Lavandula Augusti-folia, which is also known as English lavender. Lavender oil possesses strong antioxidant, anti-inflammatory, antibacterial, and antimicrobial properties, and can be used to treat various skin diseases(e.g., eczema, ringworm, acne), improve the digestive system, minimize sore muscle swelling, and additionally, alleviate pain [157,158]. Citrus limon EOs are used as antimicrobial and antifungal agents, pain relievers aid in weight loss, and to reduce extreme nausea as well as for usage as soaps, hair shampoo, furniture polishes, and fresheners [157,158]. Oregano(Origanum vulgare)EOs are often used for skin care, menstrual problems, stomach problems, and to control flu and cold infections [157,158]. Rosemary's EO originates from the Rosmarinus officinalis evergreen shrub with characteristics of a crisp woody, herbal and balsamic odor, similar to camphor. The usage of rosemary oil ranges from various treatments for skin care, dandruff, and scalp health, as well as to cold prevention and boosting the immune system [157,158]. EO from Mentha piperita is called peppermint oil, which is mainly used in the prevention of flu and colds, reduces headache symptoms, and also in relieving muscle and joint pains [157,158].

KSL25

Please click here to know more

Apart from the general usage of EOs, their extensive benefits have also been noticed and reported in relevance to age-related neurodegenerative disorders. Based on available studies, EOs have been proposed as an effective preventive and treatment approach for anti-aging and neurodegenerative disorders. Therefore, we attempted to describe and highlight the various EOs, and the effectiveness of their components with respect to the four common neurodegenerative diseases (AD, PD, HD, and ALS), as mentioned above. The different parameters that are commonly used for the evaluation of each disease are explained, accordingly.

Based on Table 1, a total of sixty-nine types of EOs from different genera of plants were evaluated for their effectiveness against neurodegenerative diseases in studies conducted between 2010 and 2020. In reference to all the compiled literature, we observed that the ICs0 results were presented in several formats, in particular to the units of measurement. cistanche herba Among the units that were reported included mg gallic acid equivalents/g[64,76], percentage [65,74], mg/mL or mg/L[26,67,68,73,75], um [69], and ug/mL [18,70-72]. This diversity, however, was considered troublesome because direct comparison among studies with different measurement units is not possible without conversion.

4.2. Major Component of EOs

The chemical structures of several major components commonly found in EOs that have been reported to have anti-neurodegenerative properties are presented in Figure3. Based on our review,18-cineole has been identified as one of the major components found in various types of EOs. The compound 1,8-cineole is a saturated monoterpene that can originate from several plant species(e.g., Eucalyptus, Rosmarinus, and Salvia), with Eucalyptus leaves recognized as the key source[159]. Sometimes called eucalyptol due to its natural source, 18-cineole should not be confused with eucalyptus oil, a combination of many other components [160]. Due to its excellent aroma and taste,18-cineole is mostly used in fruit, fragrances, and cosmetics. Furthermore, pure monoterpene 1,8-cineole is used as an alternative sinusitis remedy for respiratory tract infections, such as common cold or bronchitis [161]. It was indicated as one of the most potent free radical scavengers that may influence anticholinesterase activity based on a study reported by El Euch and colleagues [88] The antioxidant activity was measured using the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH)test. Essential oil concentration providing 50% inhibition(ICs0)of the initial DPPH concentration was calculated using the linear relationship between the compound concentration and the percentage of DPPH inhibition. Ascorbic acid was used as a standard. In the study by Abuhamdah et al.[66], EO extracted from the leaves of Aloysia citrodora Palau showed neuroprotective activity and a higher presence of 1,8-cineole was reported (23.66%).

In another study performed by Cutillas and his team [97] on EO from Thymus mas-china L., it was asserted that among all four compounds(αx-pinene,β-pinene, limonene, and 18-cineole),18-cineole was the best AChE inhibitor with an IC5o of 35.2±1.5 ug/mL They tested the antioxidant activity using five different methods such as the oxygen radical absorbance capacity(ORAC) assay, the 2,2'-and-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS) antioxidant method, the 2,2-diphenyl-1-picrylhydrazyl (DPPH)method, the thiobarbituric acid reactive substances (TBARS) method, and the chelating power (CHP)method. All the methods recorded that 18-cineole was one of the compounds highest antioxidant capacity. This finding was similar to several other types of research, which suggested the efficacy of 18-cineole as the dominant anticholinesterase agent from EOs of different sources [8,21,25,152].

4.3.Cholinesterase Activity in EOs

The most common criteria used in the determination of AD are related to the anti-cholinesterase activity. Cholinesterases(ChEs) are specialized carboxylic ester hydrolases that catalyze the hydrolysis of choline esters. Two types of ChE activity have been identified in mammalian blood and tissues, which are distinguished according to their substrate specificity and sensitivity to selective inhibitors. The first is acetylcholinesterase(AChE), which is systematically known as acetylcholine acetylhydrolase [172]. cistanche penis growth The second is butyrylcholinesterase (BChE), which is systematically referred to as acetylcholine acyl hydrolase [173-176]. The preferred substrate for AChE is acetylcholine(ACh), while butyrylcholine (BCh)and proper-onylcholine (PCh) are ideal for BChE [175-177].AChE activity is known to be inhibited by several compounds, with toxins and drugs as the major inhibitors [178]. AChE activity is used in verifying treatment effects, especially in AD [177].

KSL26

Cistanche can anti-aging

Both AChE and BChE possess active sites at the bottom of 20 A-deep gorges with 50%identical amino acid sequence, whereas the gorge entrance locates the peripheral site [179]. The active site for both enzymes comprises a catalytic triad, acyl-binding pocket, and choline-binding site [180]. A total of 14 aromatic amino acids are found in the active site of AChE, whereas six of these are substituted by aliphatic amino acids for BChE [181]. The binding and hydrolysis processes of bulky ligands are restricted in AChE due to the presence of phenylalanine residues in the acyl binding pocket. In contrast, these residues are substituted with two flexible amino acids that are selective for BChE and allow the binding of bulkier ligands [182]. The different mechanisms involved in relevance to the active gorge site specific for each enzyme have been investigated via molecular modeling, structure-based virtual screening, or even crystallographic studies [181-184].

Generally, the traditional Ellman assay is used with some modifications, applied for the determination of anti-cholinesterase activities ]185,186]. This technique is a simple, accurate, and rapid method of measuring ChE activity, that is based on the reaction between thiocholine with the sulfhydryl group of a chromogen such as 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB or Ellman's reagent). cistanche salsa benefits The shift of electrons to sulfur atoms yields a yellow substance called 5-thio-2-nitrobenzoic acid (TNB), which is measured by monitoring absorbance at 410 nm [187-190]. DTNB is a water-soluble compound and is useful for its fast reaction with thiocholine and minor side effects at neutral pH [185,187-191]. This technique, however, is also subject to certain limitations; it is restricted from testing antidots against organophosphorus AChE inhibitors or measuring AChE activity in samples of such treated individuals [190]. In addition to the Ellman assay, another method that can also be used for measuring ChE activities is the electrometric method of Michel [192]. This technique is applied based on pH changes that arise from H+ synthesis via cholinesterase hydrolysis [175,176,193].

4.4. Extracellular Plaque Deposits

Extracellular plaque deposits of the Aβ-peptide and flame-shaped neurofibrillary tangles of the microtubule-binding protein tau are the two hallmark pathologies required for AD patients. Familial early-onset forms of AD are associated with mutations either in the precursor protein for Aβ(APP)or in presenilin-1 (PS1)or presenilin-2 (PS2). Peptide generation pathways synthesize γ-secretase with either PS1 or PS2 as the catalytic subunit. APP is sequentially cleaved, where β-secretase first cleaves APP to release a large, secreted derivative， sAPPβ， followed by γ-secretase that cleaves a fragment of 99 amino acids (CTFβ)to generate Aβ. The process of y-secretase cleaving can be inaccurate, leading to C-terminal heterogeneity of the resulting peptide population that generates numerous Aβspecies, with Aβ1—40 of the highest abundance followed by Aβ42. The slightly longer forms of Aβ, particularly Aβ1-42, are the principal species deposited in the brain that are more hydrophobic and fibrillogenic [193]. In view of their vital role in Aβ synthesis, both β- and Y-secretase are considered as key components in anti-AD pharmaceuticals developments [193,194]. Normal pathology tests refer to the density in the affected brain regions of neuritic amyloid plaques and neurofibrillary tangles of tau protein. cistanche tubulosa dosage reddit AD diagnosis involves the presence of large neuritic plaque portions, consisting of highly insoluble Aβ in the brain parenchyma. There are also deposits of tau protein, although they occur among less common neurodegenerative disorders, especially in the absence of neuritic plaques. There are some distinctive morphological features of the neurofibrillary tangles in the various diseases and may exhibit a distinct composition of tau isoforms that vary from AD[195].

KSL27

It is not only humans which have amyloid-beta; non-human primates (NHPs) have the same Aβ sequences as humans, an almost identical APP sequence, and they overlap with related human biochemical pathways in many aspects, however surprisingly with aging they develop relatively few AD-like neuropathologies. Aged canines also develop severe amyloid deposition; canines tend to demonstrate extensive amyloid deposition from about ten years of age, unlike in aged NHPs, where it could take several decades[196]. Amyloid deposition in canines is also interrelated with age-related cognitive dysfunction [197]although little neuronal loss is detected. Due to a poor understanding of AD and the human brain complexity, it has been deduced that there is no natural animal model of the disease [198]. For the past 25 years, pharmacological and genetic AD models, as well as various animal species (primates, dogs, rodents, etc.), have been used in AD research activities[199,200]. The resurgence of interest in rats as the appropriate animal model for AD led to the usage of various types of rat models. As of current practice, transgenic mice have been extensively used in studies on AD. In any selected models, all need the introduction of some combined familial AD mutation into APP or PS1, or even in both [200].

4.5.Current EOs on AD

Salvia is the largest genus of plants in the family of Lamiaceae, with the number of species estimated to range from 700 to nearly 1000. Fifteen species of Salvia(namely, Officinalis L. Chionanthus, chrysophylla Staph, urmiensis, nemorosa L., syriaca, Ballina, cyanescens, divaricate, hydrangea, Kronenberg, macrochlamys, Nydegger, pachystachys, pseudeuphratica, and ruselliii)were studied for cholinesterase inhibition assay, and happen to be the most widely studied source of EOs. Most of the researchers found that Salvia spp. were weak AChE and BChE inhibitors, except for EO from S. pseudeuplratica, which demonstrated the highest inhibitory activity against AChE， at IC50=26.00±2.00 μg/mL compared to S. cyanescens and S. pachystachys. In contrast, BChE activity did not show 50% inhibition even at the highest concentration, where also was reported to be above 80 ug/mL for S. pseudeuphratica and S.hydrangea [96]. Salvia officinalis was studied twice, in 2017 and 2019. Based on GC-MS and GC-FID analysis, the main components of S. Officinalis were a-thujone, camphor, 1,8-cineole, and β-thujone. Salvia has also been used in ex vivo-based research, using the isolated guinea pig ileum method where the major molecule, rosmarinic acid, showed significant contraction responses on an isolated guinea pig ileum. Docking results of rosmarinic acid also showed a high affinity to the selected target, AChE[136]. The author suggested the potential of rosmarinic acid to become a novel therapeutic candidate for the treatment of AD.

KSL28

Other than Salvia spp., EOs from Lavandula spp. have also been studied for the treatment of AD.L. luisieri has been found to comprise high contents of oxygen-containing monoterpenes, mainly necrodane derivatives, which are absent from any other oil. This oil was tested on the endogenous beta-site APP-cleaving enzyme 1(BACE-1) in cultured cells, being responsible for a reduction in Aβ production, with no significant toxicity. Although the study was conducted in vitro, the low molecular weight and high hydrophobicity of terpenoids are properties that provide a good chance for them to cross cellular membranes and the blood-brain barrier, an essential attribute for BACE-1 inhibition in vivo [78]. However, EO from L. Angustifolia did not give a required finding because it enhanced the Aβaggregation based on the thioflavin T method; this effect was further confirmed by atomic force microscope(AFM) imaging. EO of L. Angustifolia was also shown to counteract the increase in intracellular reactive oxygen species production and the activation of the pro-apoptotic enzyme caspase-3 induced by Aβ1-42 oligomers [23,24]. Meanwhile, EO from L.pubescens exhibited strong anti-AChE and anti-BChE effects at ICso of 0.9 uL/mL and 6.82μL/mL， respectively. Carvacrol （CAR，2-methyl-5-isopropyl phenol） was also found to be higher in L pubescent. Carvacrol was found to be abundant among EOs of the Lamiaceae family and is known for various benefits including antibacterial, antifungal, antioxidant, antinociceptive, anti-inflammatory, anti-apoptosis, and anti-cancer activities [201]. Several studies on EOs have reported that carvacrol exerts some actions on the neuronal system,

including AChE inhibition [104,202], anxiolytic [203], and antidepressant [204] properties. In addition, carvacrol has the ability to modulate central neurotransmitter pathways, such as dopaminergic, serotonergic, and y-aminobutyric acid(GABA)ergic systems [201].

Only two types of cell lines, SH-SY5Y, and PC-12 were reported to have been used in research. The in vitro toxic effects of amyloid peptides are usually examined using the human neuroblastoma-derived SH-SY5Y cell line because differentiated neuron-like SH-SY5Y cells are extra-sensitive to amyloid peptides compared to non-differentiated cells because the latter lack long neurites [205]. Z-ibutilide (Z-LIG)EOs effectively protect against fibrillar aggregates of Aβ25-35- and Aβ1-42-induced toxicity in SH-SY5Y and differentiated PC12 cells, possibly through the concurrent activation of the PI3-K/Akt path-way and inhibition of the p38 pathway[105]. Aβ25-35 represents a neurotoxic fragment of Aβ1-40 or Aβ1-42 and retains the toxicity of the full-length peptide [206]. Aβ25-35 is often selected as a model for full-length peptides because it retains both its physical and biological properties [207]. In general, declining levels of PI3K subunits as well as blunted Akt kinase phosphorylation have been observed in the AD brain, which is characterized by Aβ and tau pathologies [208].

There was also a study on the potential therapeutic effect of hybrid EO from Kushui roses. Kushi rose (R.setate ×R.rugosa)refers to a natural hybrid of cog rose and traditional Chinese rose that has been cultivated for more than 200 years [209]. In this study, transgenic worm strains purchased from the Caenorhabditis Genetics Center(CGC) were used instead of rat or mice models. They found that rose EO(REO) significantly inhibited AD-like symptoms of worm paralysis and hypersensitivity to exogenous serotonin (5-HT) in a dose-dependent manner. Although the GC-MS analysis revealed the presence of 40 components, the major components,β-citronellol, and geraniol, were found to act less effectively than the oil itself. Intriguingly, REO significantly suppressed Aβ deposits and reduced the Aβoligomers to alleviate the toxicity induced by Aβ overexpression [209].

Su He Xiang Wan (SHXW) has also been studied for its neurodegenerative remedy potential. SHOW is a distinct EO and is a patent medicine comprising borneol, styrax resin, musk, aquaria, frankincense, piper, benzoin, Saussurea, cyperus, sandalwood, clove, terminally, Aristolochia fruit, rhino horn, and cinnabar. This ancient prescription was recorded in the He Ji Ju Fang of the Song Dynasty [210]. For this plant, the researchers evaluated the effects of a modified SHXW(KSOP1009 formulation) intake on the AD-like phenotypes of Drosophila AD models, which express human Aβ1-42 in their developing eyes or neurons. They found that Aβ1-42-induced eve degeneration, apoptosis, and locomotive dysfunctions were strongly suppressed. However, Aβ1-42 fibril deposits in the Aβ1-42 overexpressing model were not affected by treatment with the KSOP1009 extract. Conversely, KSOP1009 extract intake significantly suppressed the constitutively active form of hemipterous, a c-Jun N-terminal kinase (JNK) activator, while it induced eye degeneration and JNKactivation. In Drosophila, flies with mutations that augment JNK signaling accumulate less oxidative damage and live dramatically longer than wild-type flies [211,212].

Cinnamomum zeylanicum consisting of (E)-cinnamaldehyde(CAL)(81.39%)and (E)-cinnamyl acetate (CAS) (4.20%) as the main compounds showed over 78.0% inhibitory activity in cholinesterase. In MAO-A and MAO-B inhibition assays, EOs from C.zeulanicum (96.44%,95.96%) and CAL(96.32%,96.29%) demonstrated comparable activity to rasagiline (97.42%,97.38%, respectively). Research by Murata and co-workers[69] found that Kaur-16-ene, Mizuko, and ferruginol isolated from plants had anti-AChE(IC50) activity at 640, 300, and 95 um, respectively. Even though ferruginol activity has already been highlighted before, by Gulati et al.[213], this study documented the activities of kaur-16-ene and nezukol for the first time.

Meanwhile, Citrus limon has been found to significantly lower AChE brain depression in APP/PS1 and wild-type C57BL/6L(WT) mice. PSD95/synaptophysin, the synaptic density index, was substantially improved in histopathological shifts[109]. Based on the previous analysis by other researchers, nobiletin 3',4',5,6,7,8-hexamethoxy flavone was found to be the major component of polymethoxylated flavones in citrus peels, such as C. depressa, C.reticulata, C.sinensis, and C. Limon [214,215]. Thus, nobiletin may potentially be the compound that substantially alters the development of these diseases. Other than that, Acorigraminei, which was found to be rich in β-asarone, enhanced the cognitive function of AβPP/PS1 mice and decreased neuronal apoptosis in the AβPP/PS1 mouse cortex. In addition, a substantial increase in the expression of CaMKII/CREB/Bcl-2 was observed in the cortex of AβPP/PS1 mice treated with β-asarone.

In a study conducted by Ayuob et al. [113], Ocimum basilicum up-regulated the serum corticosterone level, the hippocampal protein glucocorticoid receptor, and the brain-derived neurotrophic factor(BDNF); however, it down-regulated the neurodegenerative and atrophic changes induced in the hippocampus, which decreased after exposure to chronic unpredictable mild stress (CUMS). According to the data collected by Avetisyan and co-workers [216], the major components of O. basil include methyl chavicol and linalool. Interestingly, many linalool-producing plants are commonly used in folk medicine and aromatherapy to alleviate symptoms and treat multiple acute and chronic diseases [217,218]. Linalool is frequently used in the manufacture of fragrances for shampoos, soaps, detergents, and in pharmaceutical formulations [219,220]. Research conducted by Sabogal-Gudqueta et al. [221] found that oral administration of monoterpene linalool to elderly mice (21-24 months old)with a triple transgenic form of AD(3x Tg-AD) at 25mg/kg for three months at an interval of 48 h resulted in enhanced learning and spatial memory and increased risk assessment activity in the elevated plus-maze. Hippocampi and amygdalae from 3x Tg-AD linalool-treated mice also showed a large reduction in extracellular β-amyloidosis, tauopathy, astrogliosis, and microgliosis, as well as pro-inflammatory marker levels of p38 MAPK, NOS2, COX2, and IL-1β. Thus, linalool is suitable as a prevention candidate for pre-clinical studies. Based on the articles that we have selected, linalool is a major volatile component of EOs in a number of aromatic plant species, such as L. Angustifolia Mill, M.officinalis L., R.officinalis L., and C.citrate DC. The presence of linalool can also help to reduce the deposits of Aβ, based on a study conducted by Gradinariu et al. [114]where Aβ1-42-treated rats exhibited the following: a decrease in exploratory activity (crossing number); a smaller percentage of time spent and fewer entries in the open arm in the elevated plus-maze test; increase in swim time and decrease in the immobility time within the forced swimming test.

4.6. Current EOs on PD

In terms of PD, the current therapy in practice is applied as a combination of gold-standard dopaminergic reposition with 3-(3,4-dihydroxy phenyl)-L-alanine (L-dopa), along with other agents such as MAO-B, catechol O-methyltransferase(COMT) inhibitors, dopaminergic agonists, and cholinergic blockers [222]. However, the available treatments are subject to consequences of motor and non-motor side effects, which leads to poor efficacy in advanced stages of PD[144]. These arising phenomena are the main reasons that suggest and emphasize the necessity for the synthesis of anti-PD drugs that could delay the progression of neurodegeneration [144]. As mentioned in the results, PD studies included in this review comprise in vitro and in vivo, as well as combinations of in vitro with in vivo or ex vivo research. Cinnamomum sp., Eryngium sp., Myrtus sp., Acorus sp., Eplingiella sp., Foeniculum sp., Pulicaria sp., Rosa sp., Zingiber sp., and Lavandula sp. were among the identified EOs based on the respective included PD studies.

Four of the included studies on PD were based on in vitro approaches, with various EOs. The first study was focused on the evaluation of the protective effects of EOs extracted from Cinnamomum sp.(C.verum and C. cassia) and cinnamaldehyde, in comparison to hydroalcoholic extracts using 6-OHDA-induced PC12 cytotoxicity as the representative model of PD[138]. Cinnamomum sp., more commonly known as cinnamon, belongs to the Lauraceae family that is composed of almost 250species and has been acknowledged for extensive health benefits [223,224]. Among the various species, C.verum and C.cassia were the two main species that have been widely applied for their medicinal and culinary applications, especially in Iran [225-227]. It is important to note that cinnamaldehyde represents one of the key components of both species, and EOs were reported to exhibit strong antioxidant properties [223]. The findings of this study indicated that 6-OHDA led to cell death, cell apoptosis, and suppression of the p44/42 pathway. On the whole, the study concluded that synergistic effects of cinnamaldehyde and EOs as well as other extract components could promote cinnamon's roles as neuroprotective agents, specifically for PD treatment [138].

Eryngium sp. belongs to the Apiaceae family and is recognized for its EOs' potentials in MAO inhibition [139]. MAOIs are available in two forms(A, and B) where MAO-A inhibition is linked to antidepressant effects, while MAO-B is correlated with PD treatment [228,229]. An in vitro study conducted by Klein-Junior et al. demonstrated the assessment of Eryngium sp.(E. floribundum: EP, E.horridum: EH, E.pandanifolium: EP, E. Eriophorum: EE, and E. nudicaule: EN)EOs for their MAOinhibitory effect. Intriguingly, the findings of this study indicated that MAO-A activity was not inhibited by any EOs, while EPEO and EHEOresulted in MAO-B inhibition. The literature search has also highlighted that PD patients usually represent elevated levels of MAO-B which arise due to gliosis and hence contribute to the collapse of the dopaminergic system [229]. Thus, this study puts forward the claim that Eryngium sp. could have potential applications as CNS bioactive secondary metabolites, particularly for neurodegenerative disease, in relevance to characteristics exhibited by EHEO [139].

Another important aspect that is usually associated with studies on PD is on α-Syn fibrillation. It is presumed that protein structural modifications resulting in amyloid fibril formation progress toward neurodegenerative disorders[140]. However, the exact factors of α-Syn actions of misfolding and aggregation in the brain are still scarce. In addition, it should also be noted that preventive measures against a-Syn fibrillation are yet to be available; hence, the acceleration of the fibrillation process via certain factors should be avoided. Among some of the common factors are metal ions, small molecules, nanoparticles, and, particularly, toxins that could intensify the aggregation process [140,230-233]. The next two in vitro studies were performed by the same research team, where they highlighted the effects of 15 various Iranian EOs against α-Syn fibrillation [140,141].

Among all the 15 oils tested, it was shown that M.communis demonstrated potential benefits because it elevated the fibrillation in a concentration-dependent manner. However, it is necessary to understand that the major components of this oil are not responsible for the observed changes, suggesting the complexity of both extract and synergistic effects of the available compounds, regardless of their amount [140]. In the second study, the investigation of C. cyminum EO signified the presence of cumin aldehyde as the major active compound that plays its role in the inhibition of α-Syn fibrillation. In addition, cytotoxicity assays on PC12 cells indicated the absence of toxic effects with cumin aldehyde treatment throughout o-Syn fibrillation [141].

Apart from in vitro studies, PD studies are also extensively performed under in vivo conditions. EOs of Acorus sp. cover two of the in vivo studies in this review. Both of these studies were conducted by the same research team and focused on the regulation effect of β-asarone that was isolated from A. tatarinowii Schott on 6-OHDA-induced parkinsonian rats via two distinct endoplasmic reticula (ER)stress pathways [142,143]. ER is known for its role in protein folding, where the build-up of protein unfolding/misfolding could initiate a phenomenon called ER stress that further activates the cellular process of unfolded protein response(UPR) [234]. ER stress has been noticed in a number of PD experimental models and is also provoked by an increase in wild-type α-Syn [235-237]. Three main pathways that are categorized as UPR are inositol requiring enzyme 1 (IRE1), protein kinase RNA(PKR)-like ER kinase (PERK), and activating transcription factor 6 (ATF6)[238]. In general, GRP78 functions in the regulation of ER stress, where it binds the three proteins of the UPR pathway and maintains them as inactive when the cells are not exposed to stress conditions. However, under conditions of accumulated protein unfolding or misfolding, GRP78 will bind to the proteins and release them [239-241].In terms of autophagy, the latest research has claimed that it could be induced due to ER stress [242,243]. Beclin-1 is known for its role in forming autophagosomes and is an essential part of the initial autophagy process. The pro-autophagic role of Beclin-1 could be inhibited via its reaction with Bcl-2, however, this interaction is also subject to disruption caused by Bcl-2 phosphorylation that leads to Beclin-1 release and accelerates autophagy [24]. As per the second pathway study, it was proven that β-asarone leads to Beclin-1 downregulation, which highlights that Bcl-2 could possibly be the main linkage between autophagy and ER stress. The findings of both studies lead us to conclude that diminishing ER stress via β-asarone regulation is proven to be useful in the impairment of PD pathological progression[142,143].

Another study that also applies the use of 6-OHDA-induced PD mouse models focused on the investigation of the effects of zingerone and eugenol on dopamine concentration, behavioral changes, and antioxidant activities upon 6-OHDA administration and treatment of L-dopa [28]. Zingerone is extracted from the ginger root, while eugenol originates from cloves and was reported to be protective against 6-OHDA-induced depletion of striatal dopamine via increases in SOD activity and elevation of reduced glutathione(GSH) and L-Ascorbate(Asc) concentration, respectively [245,246]. Although these groups of researchers previously reported positive findings where pre-treatment with zingerone or eugenol inhibited 6-OHDA-induced dopamine depression by preventing lipid peroxidation, the current study, which involved post-treatment with similar compounds, resulted in contradictory findings, where dopamine decrease was more pronounced [28,246]. Despite the availability of other findings that propose the benefits of consuming these compounds, Kabuto and Yamanashi [28] suggested that intake of these specific substances upon the onset of PD symptoms should be more carefully monitored to prevent further injury aggravation. In addition to studies among 6-OHDA-induced Parkinson's rat models, the possibility to achieve positive effects of EOs when complexed with β-cyclodextrin (βCD) was evaluated by Filho and colleagues using reserpine-induced progressive models for PD in mice [144]. Cyclodextrins are cyclic oligosaccharides that could form host-guest complexes with hydrophobic molecules and were also reported to protect EOs from heat, evaporation, moisture, oxidation, and light effects along with facilitating easy solubility [249]. Complexation effects of cyclodextrins with EOs were shown to be more prominent in exerting positive effects, especially in the treatment of chronic diseases, as published by several studies[250,251]. In this particular study, the same approach was applied using leaf EO extracted from Eplingiellafruticosa (EPL), where one of the key components is 1,8-cineole. Eplingiella sp. belongs to the Lamiaceae family and was reported for its benefits as anti-inflammatory and antioxidant effects [252,253]. This research demonstrated and proved the hypothesis whereby both treated groups of EPL and EPL-βCD deferred reserpine effects on catalepsy time. However, this effect was noticed to be more remarkable with EPL-βCD treated mice groups.

Another study also applied induction with reserpine, with a different PD model of ovariectomized and non-ovariectomized rats[145]. Reserpine is known as an irreversible inhibitor of the vesicular monoamine transporter 2(VMAT-2). The approach of reserpine injection to rats as a model of PD model was proposed in response to its action on the depletion of monoamine and locomotor activities [254]. Ovariectomized rats are subjected to estrogen deficits, similar to surgically menopaused women, where cognitive damage is highly possible[255]. Lower estrogen levels are correlated with many side effects, such as mental disorders, memory defects, emotional issues, and other cognitive failures [256]These incidences have led to much attraction towards phytoestrogens for their protective nature against certain diseases. Fennel plant (Foeniculum Vulgare) is classified in the Apiaceae family, and is known for its phytoestrogen compounds; it showed promising results in the treatment of cognitive disorders, such as dementia and AD [145,259]The evaluation of this study indicated that protective estrogen effects against neurodegenerative disorders were significantly decreased among reserpine-induced ovariectomized rats. Injection of reserpine resulted in a more remarkable observation of limb movement disorder among ovariectomized rats. Fennel treatment at various doses for both groups gave better results in motor activity, which stressed the importance of estrogens and phytoestrogens as a protective measure of dopaminergic neurons and improved PD symptoms[145].

Rotenone administration to rats, as induction of a PD model, is an alternative approach where it induces nigrostriatal dopaminergic neuron degeneration that is associated with o-Syn Lewy bodies [260]. Rotenone is an insecticide with high lipophilic nature and is known to inhibit mitochondrial complex-1 along with causing oxidative stress[261,262]This rotenone-induced model was reported in the study by Issa and colleagues, based on the neuroprotective effects of Pulicaria undulata EO in male Wistar rats[146]. P undulata belongs to the Asteraceae family, which is commonly distributed in Asia, Europe, and North Africa [263]. From this study, it was shown that the EO of P. undulata could exert its neuroprotective effects via anti-inflammatory and antioxidant properties. The mechanisms involved in neuroinflammation suppression include downregulation of induced nitric oxide synthase (iNOS) expression, followed by lower gene expression of o-Syn [146]. Compared to individual studies, there are also several approaches that examine combined effects that could incorporate in vitro, in vivo, and also ex vivo applications. One such attractive research is on the combined in vitro/in vivo evaluation of SHXW EO with 1-methyl-4-phenyl-1,2.3,6-tetrahydropyridine (MPTP)-induced PD mice and SH-SY cell lines [147]. In this study, SHXW was a Chinese herbal formulation that consisted of 15 crude herbs called KSOP1009, composed of eight medicinal plants of different families (Hamamelidaceae, Myristicaceae, Umbelliferae, Santalaceae, Piperaceae, Myrtaceae, Ty--phaceae, and Lamiaceae). MPTP is known to cause fast degeneration of dopaminergic neurons, and as such, it was believed that the use of this specific model could assist in explaining certain aspects of PD disease mechanisms[147,264]. Positive findings of the study demonstrated that ingestion of KSOP1009 was successful in the protection of MPTP toxicity, where which could be correlated with dopamine reduction that also decreases ROS and restores mitochondrial roles [147].

In terms of in vivo and ex vivo combinatorial approaches, two articles were highlighted in this review, with several authors originating from the same team [148,149]. Both studies explored the approach of L-dopa induction of oxidative toxicity. L-dopa has been recognized as the most effective symptomatic treatment of PD for more than 30 years; however, toxicity issues that were raised via in vitro studies seem to be an unresolved challenge [148]. It was also mentioned that prolonged L-dopa treatment is often associated with side effects that often result in a delay in its administration [148]. Past studies have claimed that L-dopa therapy in combination with antioxidants could lessen the possible side effects. As such, efforts were taken to evaluate the effects; this research team investigated the combined effects of EOs from Lavandula angustifolia, Rosa damascena, vitamin C and Trolox in the initial study [148], followed by another study in 2019 on the combined effects of pre-treatment with Rosa damascena and vitamin C [14].

For the first study, the obtained results indicated that both EOs from herbal plants showed noticeable radical scavenging and antioxidant properties against L-dopa toxicity. Similarly, the second study also put forward equivalent claims with R. Damascena characteristics where it was in parallel to vitamin C and exhibited a significant role of rose oil in its interference against the acute oxidative toxicity of L-dopa [149]. Based on all the collective studies, it could be observed that PD treatments remain centralized among several parameters that include mainly a-Syn fibrillation, MAO-B, β-asarone regulation of ER stress pathways, toxicity-induced models with 6-OHDA, MPTP, L-dopa, reserpine, and rotenone with common animal models of rats and mice. Although the regulatory mechanisms involved in each of the parameters may differ, the main focus remains on effective and improved treatments for PD patients. disorder among ovariectomized rats. Fennel treatment at various doses for both groups gave better results in motor activity, which stressed the importance of estrogens and phytoestrogens as a protective measure of dopaminergic neurons and improved PD symptoms[145].

This article is extracted from Molecules 2021, 26, 1107. https://doi.org/10.3390/molecules26041107 https://www.mdpi.com/journal/molecules