The Natural Antioxidant Cistanche Deserticola Scavenging Free Radicals
Mar 10, 2022
Contact: emily.li@wecistanche.com
Effect of cistanche deserticola on lipid peroxide content in brain and liver of aged mice
Ren Chunqing, Jiang Fu, Zhu Qiushuang, Wang Jun, He Lihua, and Su Bo
Abstract: Different doses of cistanche deserticola can be given to mice by intragastric administration for 4 weeks, which can significantly reduce the content of lipid peroxides in the liver. The results suggest that cistanche deserticola scavenging free radicals has obvious antioxidant effects and can effectively reduce lipid peroxidation.
Introduction
A free radical can be defined as any molecular species capable of independent existence that contains an unpaired electron in an atomic orbital. The presence of unpaired electrons results in certain common properties that are shared by most radicals. Many radicals are unstable and highly reactive. They can either donate an electron to or accept an electron from other molecules, therefore behaving as oxidants or reductants.[5] The most important oxygen-containing free radicals in many disease states are hydroxyl radical, superoxide anion radical, hydrogen peroxide, oxygen singlet, hypochlorite, nitric oxide radical, and peroxynitrite radicals. These are highly reactive species, capable in the nucleus, and in the membranes of cells of damaging biologically relevant molecules such as DNA, proteins, carbohydrates, and lipids.[6] Free radicals attack important macromolecules leading to cell damage and homeostatic disruption. Targets of free radicals include all kinds of molecules in the body. Among them, lipids, nucleic acids, and proteins are the major targets.
Generally, free radicals are chemically active and can only exist instantaneously. In 1956, Denham Haman first proposed the theory of aging free radicals. Its main argument is that the degenerative changes of human beings with age are caused by the damage of free radicals and reactive oxygen species. Under physiological conditions, these free radicals are constantly produced, but they are also constantly eliminated, so the damage of free radicals to the body is not shown. Because certain physiological functions or biochemical reactions require the participation of O, OH, etc., the body also needs to maintain a beneficial, harmless, physiologically low-level, stable, and balanced free radical concentration through the generation and elimination of free radicals. The body’s antioxidant defense system has two systems: enzymatic and non-enzymatic. The former mainly uses trace elements as the active center, such as SOD, etc., which can make toxic O and H2O: disproportionation and reduction are eliminated, thereby preventing. It causes a chain reaction of free radicals. However, under pathological conditions, the production and elimination of free radicals are out of balance, whether the cause is increased production of free radicals, the body’s ability to scavenge free radicals is weakened, or both, the consequence is that the body is damaged. Accelerate aging. The decrease of the body's total antioxidant capacity, especially the decrease of antioxidant enzyme activity and the increase of peroxy lipid content are closely related to aging.
A large number of studies have shown that with the increase of age in adulthood, the content of malondialdehyde in the body increases, while the activity of SOD gradually decreases. Under the action of free radicals, the polyvalent unsaturated fatty acids (DUFA) in the biological membranes in the body produce lipid peroxides (LPO), and LPO is further decomposed to produce a large number of alcohols, aldehydes, and hydroxyls. Among them, malondialdehyde (MDA) is a second small molecule product of lipid peroxidation and decomposition. It is a highly biologically toxic substance that easily reacts with phospholipids, etc., so the active LPO can reflect body lipids. The speed and intensity of peroxidation reflect the degree of cell damage (P, this experiment is to further study the mechanism of action of Cistanchs deserticola, so the content of LPO in the brain and liver of aging mice was determined. The results are now reported as follows.
antioxidant:cistanche deserticola
Material and methods
Medicine: The material medicine cistanche deserticola. Y.C Ma is a commercially available Chinese herbal medicine, decocted 3 times in water. Combine the three filtrates and make the concentration of the filtrate be 0.25%~0.5% (1ml contains about lg of crude drug). The standard tetras the propane is a product of Sigma Company in the United States, and thiobarbituric acid is a product of Shanghai Reagent No. 2 Factory.
Animal selection and grouping: Kunming mice are provided and raised by the animal room of our hospital. The aged mice were over 12 months old, with a total of 30, weighing 40g ± 5g, male and female, and were randomly divided into 3 groups, the cistanche deserticola administration group 2 groups and the aging mouse control group, each with 10 mice. Under the same conditions, each component was fed and the administration group was given intragastric administration once a day, and the control group was given corresponding physiological saline for 4 consecutive weeks. After the end, the organs were taken.
Determination method: Take the brain and liver to prepare 5% brain and liver homogenate physiological saline solution, use the literature method, use the 721 spectrophotometers to determine colorimetrically at 532nm wavelength, and calculate the lipid peroxide content per g of tissue. Statistical processing of experimental data (microcomputer) The experimental data are expressed as ±S, and the significance test between the means adopts the analysis of variance.
Results
The effect of Roucong Rong on the lipid peroxide content in the brain and liver tissues of aged mice. See the attached table. The results show that Cistanche deserticola significantly reduces the content of lipid peroxide in the brain and liver tissues of aged mice compared with the aged control group. , As the dose increases, the content decreases accordingly.
Discussion
The increase in lipid peroxide is the result of blood oxygen-free radicals acting on lipids [4]. With aging, the activity of superoxide dismutase in the body gradually decreases, but superoxide dismutase has the effect of scavenging blood oxygen free radicals and reducing blood lipid peroxides. All drugs that can reduce blood lipid peroxides and increase blood superoxide dismutase have anti-free radical damage [5]. Cistanche deserticola can lower blood pressure and inhibit tumor cells. This experiment confirmed that Cistanche deserticola reduced the content of lipid peroxide in brain and liver tissues in aged mice (P<0.01). It is suggested that cistanches deserticola has an antioxidant effect, can effectively reduce lipid peroxidation, reduce the content of lipid peroxide, enhance the body's scavenging activity of free radicals, reduce the damage of free radicals to the body, so it has the effect of delaying aging.
anti-free radical damage and anti-oxidant cistanche deserticola
references
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2. Jin Hui. Oxidation and Antioxidant Damage in Diabetes, Medical Review 1996, 2(2); 49
3. Riely CA, et al: Ethane evolution: a new index of lipid peroxidation, sciences 1974;183 208
4 Heinecke JW Free-Radical biology and medicine.Alan Kliss. New York1987:65
5. Roberts MJ, et al: transient release of lipids after coronary artery peroxide Baloon angioplasty Lancer 1990;21:143
