Effect Of Cistanche Phenylethanol Glycoside Intestinal Barrier And Gut Microbiota in Micealcoholic Liver Disease Ⅱ

3 results

3.1 Effect on the general condition of mice

The normal group and the normal medication group had good mental status, normal diet, smooth coat, and well-formed feces. The condition of the model group and CPhGs low, medium and high dose groups was slightly worse, with occasional unformed feces. As shown in Table 1, the body mass of the model group has declined, which is not statistically significant; compared with

Compared with the normal group, the liver coefficient of the model group decreased, without statistical significance; compared with the model group, the liver coefficient of the CPhGs low, medium and high dose groups increased (P<0.05).

NATURAL ORGANIC CISTANCHE EXTRACT WITH 40% ECHINACOSIDE AND 85% GLYCOSIDE FOR LIVER PROTECTION

3.2 Effects on serum ALT, AST, TG and TC levels in mice

As shown in Table 1, compared with the normal group, ALT, AST and TG in the serum of mice in the model group were significantly increased (P<0.01); compared with the model group, ALT, AST in the serum of mice in the CPhGs high-dose group were significantly increased (P<0.01). and TG were significantly reduced (P<0.05). Compared with the normal group, mice in the model group

Serum TC levels did not change significantly.

Table 1 Body weight, liver coefficient and serum liver function indexes of mice in each group (𝑠, n=6)

3.3 Effects on mouse liver histopathology

The HE-stained pathological sections of liver tissue were observed under an optical microscope. As shown in Figure 1, compared with the normal group and normal administration group, the livers of mice in the model group showed obvious fatty degeneration, mainly showing diffuse vacuolar degeneration of hepatocytes. A small number of inflammatory cells infiltrated; compared with the model group, the fat vacuoles of mice in the low, medium and high dose groups of CPhGs were significantly smaller and the number was significantly reduced.

The oil-red O-stained pathological sections of the liver tissue were observed under an optical microscope. As shown in Figure 2, the livers of the mice in the model group showed obvious fatty degeneration, and a large number of red lipid droplets were seen in the liver cells of the mice. This was different from the normal group and normal administration. Compared with the control group, the number of hepatocytes containing red lipid droplets was significantly increased. Compared with the model group, the number of hepatocytes containing red lipid droplets in the livers of mice in the CPhGs low, medium and high dose groups was significantly reduced.

Fig.1 Effect of CPhGs on pathological of liver in ALD mice (HE, ×400)

Fig.2 Effect of CPhGs on pathological of liver in ALD mice (Oil red O staining, ×400)

3.4 Effects on TG, TC and LBP levels in mouse livers

As shown in Table 1 and Table 2, compared with the normal group, the contents of TG, TC and LBP in the liver tissue homogenate of mice in the model group were significantly increased (P<0.01); compared with the model group, CPhGs were lower, The contents of TG, TC and LBP in the serum of mice in the medium and high groups were significantly reduced (P<0.01).

Table 2 Effect of CPhGs on related indexes of liver homogenate in ALD mice (𝑥̅± 𝑠, n=6)

3.5 Effects on serum TNF-α, IL-1β, LPS, LBP, D-LA and DAO levels in mice

As shown in Table 3, compared with the normal group, the levels of inflammatory factors TNF-α and IL-1β, intestinal endotoxins LPS and LBP, and intestinal permeability indicators DAO and D-LA in the serum of mice in the model group were higher. Significantly increased (P＜0.05); compared with the model group, the TNF-α level in the serum of mice in the CPhGs low-dose group was significantly reduced (P＜0.05); the TNF-α, IL -1β, LPS and DAO levels were significantly reduced (P＜0.05); TNF-α, IL-1β, LPS, LBP, DAO and D-LA levels in the serum of mice in the CPhGs high-dose group were significantly reduced (P＜0.05 ).

Table 3 Effect of CPhGs on serum inflammatory factors, endotoxin and intestinal permeability in ALD mice (𝑠, n=6)

Note: Figure A: Normal group, B: Normal administration group, C: Model group, D: CPhGs high-dose group.

Fig.3 Effect of CPhGs on pathological of jejunum in ALD mice

Note: Figure A: Normal group, B: Normal administration group, C: Model group, D: CPhGs high-dose group.

Fig.4 Effect of CPhGs on pathological of colon in ALD mice

Supportive Service Of Wecistanche

Email:wallence.suen@wecistanche.com 

Whatsapp/Tel:+86 15292862950

Shop For More Specifications Details:

https://www.xjcistanche.com/cistanche-shop