Effect Of Cistanche Polysaccharides On Proliferation Of Lymphocytes

Xiangyan Wang, Yun Qi, Runlan Cai, Xiaohong Li, Meihua Yang, and Yue Shi

(Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193)

Contact: joanna.jia@wecistanche.com / WhatsApp: 008618081934791

Abstract

Objective

To study the effects of Cistanche deserticola polysaccharide (CDPS) on the proliferation of lymphocytes in mice. Methods MTT method was used to detect the proliferation of mouse splenic lymphocytes. Cyclophosphamide (Cy) replicates the immunocompromised animal model and measures the spleen and thymus indexes of normal and immunocompromised animals. The thymocyte proliferation method measures the activity of interleukin-2 (IL-2). Results CDPS had a significant proliferation effect on mitogen (ConA and LPS) activated lymphocytes and non-activated normal cells and promoted the secretion of lymphocyte IL-2. Intraperitoneal administration showed that CDPS can significantly increase the spleen index of normal and immunocompromised mice, and it also has a significant antagonistic effect on the decrease of thymus index caused by Cy. Conclusion CDPS can significantly promote the proliferation of mouse splenic lymphocytes, which may be related to the promotion of IL-2 secretion.

Keywords: Cistanche polysaccharide; spleen index; thymus index; lymphocyte proliferation; interleukin-2

Cistanche polysaccharides have many functions

Plant polysaccharides have attracted widespread attention due to their universal immunomodulatory effects and their relatively low toxicity characteristics [1]. Cistanche deserticola is a dry fleshy stem with scales of Cistanche deserticola Y.C.Ma, which has a wide range of pharmacological effects. Its decoction can significantly increase the phagocytic percentage and phagocytic index of macrophages [2], and further studies have shown that its material basis is Cistanche deserticola polysaccharide (CDPS) [3], Zeng Qunli et al. [4] found CDPS A single component in thymus lymphocytes in vitro has a significant proliferation effect on mouse thymus lymphocytes. This article observes the effects of CDPS on (spleen) lymphocyte proliferation in vivo and in vitro.

Cistanche polysaccharide has the effect of improving immunity

Discussion

ConA and LPS induced the proliferation of mouse splenic lymphocytes are in vitro experiments to detect the function of T and B lymphocytes in the body, respectively, and are important indicators to reflect specific immunity. Studies have shown that when the final concentration of CDPS is above 25 mgL, it can significantly promote the proliferation of lymphocytes, showing that it has a certain mitogenic effect. In the presence of mitogens (ConA or LPS), a smaller dose of CDPS can show obvious proliferation-promoting activity. We know that ConA and LPS mainly induce the proliferation of T lymphocytes or B lymphocytes, respectively. Studies have shown that CDPS can not only promote the proliferation of T lymphocytes, but also promote the proliferation of B lymphocytes, but it has a significant effect on the proliferation of B lymphocytes. For T lymphocytes.

Cistanche Polysaccharides: Improve Immunity

The thymus and spleen are the main immune organs in animals. The immune cells in the spleen are mainly mature B lymphocytes, and the thymus is mainly immature T lymphocytes. The thymus and spleen index can reflect the development of immune organs and the functional status of immune cells. Intraperitoneal injection of CDPS can significantly increase the spleen index of normal and immunosuppressed mice, and it also has a significant antagonistic effect on mouse thymic atrophy caused by Cy. CDPS has no significant effect on the thymus index of normal animals, which is consistent with the results of in vitro experiments that CDPS promotes the proliferation of B lymphocytes than T lymphocytes.

Cistanche polysaccharide: increase the proliferation of lymphocytes and improve immunity

IL-2 is a cytokine produced by T lymphocytes from the G1 phase of the cell cycle to the S phase. It is a major T cell growth factor and acts as an autocrine growth factor. In addition, IL-2 can promote B cell proliferation and antibodies Synthesis [11, 12]. CDPS promotes the release of IL-2 from lymphocytes, and this effect is obviously related to CDPS's promotion of splenic lymphocyte proliferation.