A Tandem Segmentation-classification Approach For The Localization Of Morphological Predictors Of C. Elegans Lifespan And Motility

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Abstract

C. elegans is an established model organism for studying genetic and drug effects on aging, many of which are conserved in humans. It is also an essential model for basic research, and C. elegans pathologies is a newly emerging field. Here we develop a proof-of-principal convolutional neural network-based platform to segment C. elegans and extract features that might be useful for lifespan prediction. We use a dataset of 734 worms tracked throughout their lifespan and classify worms into long-lived and short-lived. We designed WormNet- a convolutional neural network (CNN) to predict the worm lifespan class based on young adult images (day 1-day 3 old adults) and showed that WormNet, as well, InceptionV3 CNN can successfully classify lifespan. Based on U-Net architecture we develop HydraNet CNNs which allow segmenting of worms accurately into anterior, mid-body, and posterior parts. We combineHydraNet segmentation, WormNet prediction, and the class activation map approach to determine the segments most important for lifespan classification. Such a tandem segmentation-classification approach shows the posterior part of the worm might be more important for classifying long-lived worms. Our approach can be useful for the acceleration of anti-aging drug discovery and for studying C. elegans pathologies.

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INTRODUCTION

The nematode Caenorhabditis elegans (C. elegans) is an established model for studying various interventions into the aging process, which allowed to find numerous genes and drugs interfering with aging. 5 out of7 Tier 1 and 4 out of 6 Tier 2 anti-aging drugs considered for human trials extend lifespan in the C. elegans model. cistanche แอ ม เว ย์ There are many aging pathways conserved among species and the worms are expected to be used extensively not only in longevity research but also in the appearing anti-aging industry [1]. Additionally, humanized worms are now used to establish promising models for neurodegeneration [2]. However, unlike genetics of longevity, C. elegans phenotypes of aging are not well studied yet. Particularly, we know little about age-related pathologies and their development, as well as, which pathologies determine lifespan and how they cause death [3]. Several pathologies including gut atrophy, uterine tumors and pharyngeal infection were described recently [4-6]. In this light, discovering new C. elegans pathologies, particularly determining lifespan, is becoming an important challenge. Studying pathologies in C. elegans might help to get a better understanding of the aging process, as well as, the mechanisms and effects of anti-aging drugs.

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Recent advances in machine learning (ML) and deep learning (DL)[7] may aid aging studies employing C.elegans by uncovering and summarizing previously unseen behavioral and morphological patterns in large experimental datasets. For example, in a recent work several physiological parameters were measured longitudinally and an application of support vector regression allowed to explain the different amount of variance in C. elegans lifespan by: movement (57%), cross-sectional autofluorescence(52%), oocyte laying rate (28%)[8]. Interestingly, it was found that the brood size correlates with lifespan in mated hermaphrodites (r = 0.28)[9]. Furthermore, independent studies confirm that the muscle function is probably the best predicting physiological feature: fast pharyngeal pumping span (r=0.49), and pharyngeal pumping span(r=0.83) were found to be highly correlated with lifespan length [10]. Also, maximum velocity at day 9[11] and the rate of speed decay (days 3-9)[12] predict 71% and 91% of the variability in lifespan accordingly. Cellular and molecular predictors of C. elegans lifespan length were also discovered. Expression of hsp-16.2 induced by heat shock in day 1 adults was found to be correlated with lifespan [13]. Free of confounding effects of interventions like heat shock, basal expression of sod-3 at day 9 also correlated with lifespan (r = 0.57), which probably reflects response to pathogenic food [14]. Mir-71 expression from day 4 onwards can be highly predictive and explains 47% of the variability in lifespan [15]. Strikingly, a strong inverse correlation (r =-0.93)between nucleolar size (measured on day 1)and longevity indicates deregulated protein synthesis as an important component of aging[16]. Noteworthy, early on a Machine Vision approach was also applied to classify aging phenotypes in C. elegans. Particularly, linear discriminant classifier was used to segregate images of pharynxes of different ages for subsequent molecular characterization [17].

Among other methods, one of the most powerful machines learning approaches, particularly for image analyses, is the use of convolutional neural networks (CNN)[18], which are inspired by the visual cortex neural network organization. CNN allowed to achieve impressive results in image recognition, with near-human performance on the MNIST dataset and outperformed humans on traffic sign recognition by a factor of two [19]. CNN repeatedly showed best performance during "The ImageNet Large Scale Visual Recognition Challenge"in image classification [20,21]. how much cistanche to take The introduction of skipped connections to CNN dramatically improved their speed and accuracy, and such residual CNNs are now state-of-the-art for image classification [22, 23]. Encoder-decoder residual networks like U-Net [24], V-Net, and Tiramisu also outperform the classical boundary extraction, threshold, and region-based methods used in the medical image segmentation field [25]. Despite the impressive results with DL approaches, one of the main drawbacks is that DL networks are black boxes so it is difficult to get the features important for decision-making by the network [26]. To circumvent this shortcoming, several saliency techniques have been proposed [27-29]. One such technique is using the global average pooling layer to produce a so-called class activation map (CAM) and localize class-specific image regions in an unsupervised manner [30]. The produced generic localizable deep features can aid researchers in understanding the basis of discrimination used by CNNs for their tasks. However, thus far, no approaches to combine biologically meaningful image segmentation and classification saliency to facilitate phenotype discovery through interpretation have been developed.

Remarkably, CNN were recently used to predict lifespan in worms. In the first paper, a dataset of 913 images of C. elegans were used. Each time point (day)has at least 30 worms, and all of them were anaesthetized before imaging. InceptionResNetV2-based architecture achieved a mean absolute error (MAE) of 0.96 day in the regression mode, and an accuracy of 57.6% in classification mode [31]. In another work, the authors used an automatic imaging system capable of tracking the same worm during the whole lifespan, so they had data for 734 worms for which images were taken every 3.5 hours. They used U-Net to segment worms from the background and then performed the worm body coordinate regression to create straightened worm representations. Then they used a modified ResNet34 and managed to regress worm age with minimal MAE of 0.6 days for raw images [32].

Here we used the same dataset as in [8, 32], however instead of predicting age of each worm, we develop a CNN-based platform we called WormNet capable of classifying young adults (day 1-3) into short-lived and long-lived, and also design an approach for extracting features important for such classification. Similarly, we have applied WormNet to classify C. elegans movement. To interpret classification results in a by-design fashion, we have accompanied classification CNN with a tandem segmentation CNN. For this, we devised a new U-Net-based architecture (HydraNet) for segmenting worms from the background and also segmenting the worm's body into anterior, mid-body and posterior parts. Interpretation of the classification results was achieved through the union of HydraNet segmentation and class activation maps generated using WormNet. The class activation maps analyses combined with body part segmentation in such tandem fashion allowed us to extract features responsible for lifespan prediction. Finally, using a higher resolution segmented version of the C. elegans images, we verified our results in a higher expressive capacity residual CNN InceptionV3 accompanied by manual interpretation.

RESULTS

The time-lapse data for 734 C. elegans captured from day 1 of adulthood till death were used to develop our prototype platform [8,15]. To develop an approach for automated interpretability of these images we addressed a problem of segmenting the worms from their background, as well as distinguishing worms'morphological parts (Figure 1). For this, we have manually annotated 130 images of adult worms with masks for anterior, mid-body, posterior parts of the worm and summing up to a total worm mask (Figure 1F-1H). This dataset was then split into the train (90)and test (40) fractions based on the dataset ID of an individual worm to ensure that individual worm features would not leak to the test hold-out. First, to address the total worm segmentation problem we have constructed a relatively shallow architecture akin to U-Net [24]accompanied with a sigmoid head for binary classification. For clarity, the encoding and decoding parts of U-Net are shown on Figure lA as a and β. The raw images were scaled to 96 × 96 pixels for computational efficiency. We used the Dice loss function and monitored Jaccard index to assess the segmentation quality. On this relatively simple segmentation problem Jaccard index reached 0.97 on both train and test fractions (Figure 1A, 1B, see Materials and Methods for detailed hyperparameters). Next, to extend this approach to segmentation of individual body parts of C. elegans we have reformulated the problem as a multi-class segmentation with one-hot encoded masks and similar U-Net-like architecture (Figure 1C, 11). Unsurprisingly, since a multi-class classification is a harder problem, this led to a worse performance of 0.92 and 0.91 Jaccard index on train and test fraction respectively suggesting a mild overfit.

Remarkably, one aspect of multi-class U-Net did not perform well was distinguishing anterior and posterior parts of the worm which led to generating overlapping masks (Figure lI). To circumvent this limitation, we have designed an alternative architecture using U-Net a and β parts, with multiple β parts dedicated each for its own binary segmentation problem (Figure 1D, 1E), which we called HydraNet. Such approach creates a jointly trained architecture with common input layers and layers dedicated for each of the morphological parts of the worm, allowing to have an end-to-end model, while solving a simpler binary classification problem. HydraNet3 was equipped with 3 β parts dedicated to the anterior, mid-body, and posterior parts of the worm body. HydraNet4, in turn, was equipped with 4 parts dedicated to the anterior, mid-body, posterior parts as well as the whole worm body. To estimate joint performance of HydraNet we measured Jaccard index for each β part individually and finally evaluated the average Jaccard index. Remarkably, both HydraNet3 and HydraNet4 achieved the average Jaccard index 0.97 on both the train and test fractions demonstrating good generalization (Figure 1D, IE, 1J, 1K). Noteworthy, HydraNet4 achieved conversion earlier than HydraNet3 (Figure 1D, IE insets) suggesting a potential positive effect from accompanying the architecture with a more general semantic class.

Next, to obtain classifiers for C. elegans movement or lifespan, we split all 734 worms into 2 total movement amount classes: low or high movement estimated as motility above or below average distance crawled during the life-time; and 2 lifespan classes: 'short-lived'with lifespan 7 days or less, and 'long-lived' with lifespan 8 days and more. The task was to predict classes based on day l, day 2 or day 3 images. As the dataset is relatively small, the use of high expressive capacity architectures could lead to overfitting. Therefore, we designed a relatively shallow CNN we called WormNet. This architecture consisted of 5 convolutional layers, each followed by a max pooling layer. Dropout and batch normalization were implemented for each convolutional layer in the neural network to improve generalization. what is a cistanche The last max pooling layer was flattened and attached to a fully connected layer followed by a softmax layer. We used binary cross-entropy as a loss function. All the layers, except the latter one, used a rectified linear unit (ReLU) as an activation function (Figure 2A, see Materials and Methods for detailed hyperparameters). WormNet was used to obtain both movement and lifespan classifiers (Figures 2 and 3). To further alleviate potential overfitting, we performed a 30-fold data augmentation using Keras image generators. Specifically, images are subject to random horizontal and vertical flipping, horizontal and vertical shift within 10% range, as well as random rotations within 90 degrees range of the original. Blanks in the transformed images were filled using the nearest value strategy.

The WormNet showed good performance on total movement classification reaching 88% accuracy (precision 0.86, recall 0.86, area under curve for receiver operating characteristic - AUC ROC - was 0.56) on the test dataset for the day 3 adults fraction. The performance for the day 1 and day 2 images were slightly lower (Figure 2B-2D) with ROC AUC of 0.51 and 0.55 respectively. To ensure our prediction is influenced mostly by the worm morphology rather than its surroundings, we have generated a dataset of synthetic background images where C. elegans were removed through segmentation. To alleviate worm silhouette influence on the training, we have filled the remaining zero pixels with random noise (Supplementary Figure 1). Our results suggested that the model performance is predominantly attributed to the C. elegans morphology rather than the background of the images. To assess which body part might be responsible for the WormNet decision-making, using our tandem segmentation-classification approach we have obtained CAMs for a low movement class worm (Figure 2E,2F)and a high movement worm (Figure 2G, 2H) from WormNet. bioflavonoids Next, each image was segmented using HydraNet4 and the union of WormNet upper quartile CAM with morphological part segmentation from HydraNet4 was obtained. For interpretation purposes, we have computed the percentage of CAMs belonging to a respective morphological segment for each respective worm belonging to high or low movement class. Furthermore, we assessed the significance of this by-design interpretation using one-way ANOVA with Tukey's honest significant difference (HSD) correction (Figure 2F-low movement worms, Figure 2H - high movement worms). The comparison suggested that the anterior part was covered significantly less (31%) than the mid-body (34%) and posterior parts (34%) for both low and high-movement worms. There was no significant difference between the mid-body and the posterior part of the body.

Next, we used WormNet to classify long and short-lived worms. Similarly to movement classification, the WormNet performed better on day 3 adults sample reaching an accuracy of 72%(precision 0.73, recall 0.71, AUC ROC 0.61) on the test dataset, as compared to AUC ROC of 0.53 and 0.52 for day 2 and 1 respectively. buy cistanche The confusion matrix analysis suggested that the CNN underperformed in short-lived worms classifying (Figure 3A-3C). Next, we have interpreted the classifier using the tandem of HydraNet4 and WormNet accompanied by a one-way ANOVA statistical test. In the case of lifespan classification, by-design interpretation suggested that at 32% the anterior part was significantly less pronounced in CAMs compared to the mid-body and the posterior part (Figure 3D, 3E-short lifespan, Figure 3F, 3G-long lifespan). This difference was less significant for long lifespan than for short lifespan. There was no significant difference between the mid-body and the posterior part.

To verify these findings in an independent manner we have trained another lifespan classifier using the residual InceptionV3 architecture [3] accompanied by a manual interpretation (Figure 4). Furthermore, in this case to ensure high resolution of the CAMs instead of scaling to 96×96 pixels, the full resolution 900×900 images cropped to 800×800 pixels（516×516 μm）were used. As a much higher expressive capacity CNN, InceptionV3 was prone to overfitting on our relatively small dataset (Figure 4C, 4D). To circumvent this, we have implemented early stopping during training. Additionally, we segmented the worms from their background ensuring InceptionV3 is presented only with the relevant part of the image. InceptionV3 performed similarly to WormNet with the accuracy reaching 70% on the test dataset for lifespan classification (Figure 4A). Consistently with the tandem HydraNet4-WormNet approach to interpretation, in the case of the manual interpretation, the anterior part of the worm was highlighted by the InceptionV3 CAM less frequently. Importantly, however, due to the higher resolution of the input images, the CAMs now localized the body parts much better, allowing to assign a body part as a possible discriminator in each case (Figure 4B). Interestingly, the distribution of the body parts highlighted by CAM's analysis demonstrates that the posterior part is more important for long-living worms'classification, suggesting that the features predicting longevity could be located in the posterior part of the worm body.

DISCUSSION

Despite C. elegans being a classical model in aging research with more than 4000 papers published up to date, and the progress in robotics, the process of measuring C. elegans lifespan is still manual and laborious. However, new approaches are emerging like lifespan machine utilizing flatbed scanners to simultaneously assess the viability of a large population of worms on plates [34]. Another approach is Worm corals-an automated vermiculture method allowing to track worms throughout their lifespan with much better detailed measurements [8]. The detailed physiological data produced on Worm corals showed that movement, autofluorescence and textural degradation are the best predictors of lifespan. However, it remains unclear what exact morphological features reflect pathologies and determine the lifespan length. It was also found that physiological measurements before day 3 or 4 of adulthood and single GFP labelled biomarkers cannot distinguish short and long-lived worms [8,15]. Nucleolar-based predictions made on day l adults are performed using 100× magnification on fixed worms, which is not achievable for any automated screening platform.

Here we worked with the dataset generated in Pincus lab [8,15], and showed that the application of newly designed WormNet was able to successfully discriminate between short and long-lived worms even for images taken at day 1 or day 2; importantly, for day 3 the CNN demonstrated the best performance (Figure 2A-2C). WormNet was even better at classifying worms with high and low total movement, achieving 88% accuracy for day 1 adults (Figure 3). We expect that generate more data and developing the CNN predict lifespan [32]. As mentioned earlier, the authors segmented the worms and created straightened worm representations, which were used for CNN training [32]. Increased number of samples improved the regression-based prediction of worm age. Interestingly, the authors has limited found t silhouette alone that worm information for age estimation, whereas the information background from improve the can significantly accuracy, though the predictive value of background is an artifact of experimental conditions. Therefore, it might be possible that the predictive accuracy of WormNet in our simulations can be partly explained by the background information. However, as our experiments suggest (Supplementary Figure 1), WormNet performance mostly depend on the C. elegans morphology rather than the background of the images. Importantly, pretraining on the body-coordinate representations in [32] improved accuracy on raw images which suggests that worm organs and texture are useful for age prediction.

In addition to lifespan or movement classification based on young adults' images, we also aimed to find features important for the prediction. As a prototype task we decided to determine which body part-anterior, mid-body or posterior part contains features influencing lifespan length the most. We designed HydraNet 3 and 4, new architectures based on U-Net and showed that they can successfully segment worm body parts achieving perfect Jaccard index values. Importantly, to develop a by-design interpretation approach we employed a tandem of biologically meaningful classification (lifespan and movement) yielding saliency through class activation maps [30, 35] and morphological segmentation (anterior, mid-body and posterior regions) to find which body part is useful for the classifications. Furthermore, although less resolved, findings obtained from the tandem approach were consistent with an independently trained classifier. This binary classifier was based on the InveptionV3 CNN. It was trained on 800×800 pixels full optical resolution images with worms segmented from their background and achieved results comparable to WormNet, though the model is less generalizable due to more overfitting (Figure 4). However, in the case of InceptionV3, distinct body parts could be localized on the CAMs, and the analyses suggest that features located in the posterior part of the worm might be more important for classifying long-lived worms. This approach provides an avenue to the discovery of new important age biomarkers in C. elegans in an automated setting, given a significant increase in image resolution and usage of body-coordinate representation. Non-labelled organs like pharynx or GFP-labelled entities could be segmented using HydraNets and assessed for their lifespan predictive ability using CAM approach and WormNet. It is tempting to speculate that akin to generative adversarial networks [36], future implementations of the by-design interpretability through a tandem of segmentation and classification may be trained end-to-end and employed for routine scientific discovery. The proof-of-principle automated analytical platform will be useful for non-invasive aging biomarkers discovery, particularly in young day 1-3 adult C. elegans. This has great potential to accelerate the pharmaceutical screening for anti-aging drugs. The development of the methodology will also be helpful to find and characterize new pathologies in C. elegans important for basic aging research. To make the code available to the research community we have deposited.

This article is extracted from www.aging-us.com AGING 2022, Vol. 14, No. 4