Analysis Of The Medication Rule Of Cistanche Compound Patent in The Treatment Of Male Infertility
Apr 15, 2022
Abstract: Objective Through the excavation of the patents of traditional Chinese medicine (TCM) for the treatment of male infertility, to study the medication rules of their compatibility, and to provide a basis for clinical prescriptions and new drug development. Methods The patents of TCM compound prescriptions for the treatment of male infertility in the national patent database from 2010 to 2021 were searched, and the TCM inheritance calculation platform (V3.0) was used to make statistics on the property, and taste, meridian, efficacy, and frequency of Chinese medicines. Association rules and complex system entropy clustering methods were used to analyze the medication rules of compatibility of TCM compounds. Results A total of 123 TCM compound prescriptions for the treatment of male infertility were included, involving 220 Chinese medicines. The top 6 frequently used were Cistanche, Tusizi (Cuscutae Semen), Gouqizi (Lycii Fructus), Yinyanghuo (Epimedii Folium), Shudihuang (Rehmanniae Radix Praeparata), Danggui (Angelicae Sinensis Radix), Roucongrong (Cistanches Herba). The property of medicine involved was temperature, followed by flat and cold; most of the medicine taste was sweet, followed by bitter and pungent; kidney and liver meridians were the main meridians;
Male infertility is one of the common diseases in andrology, which refers to the fact that both husband and wife have had normal sexual life for more than 1 year without contraception, and have not given birth due to male factors alone. In recent years, with the changes in social living habits and the environment, the incidence of male infertility has been increasing year by year. The etiology of male infertility is complex. At present, although clinical
The understanding of this disease has reached the genetic level, and artificial assisted reproductive technology has gradually matured, but there are still some patients with poor curative effects. On the other hand, with the country's comprehensive liberalization of the "three-child policy", fertility indicators such as male sperm motility, quantity, morphology, and semen volume will face new challenges, requiring integrated traditional Chinese and Western medicine treatment to meet clinical needs. Male infertility belongs to the categories of "childlessness" and "sterility" in traditional Chinese medicine. Traditional Chinese medicine has rich experience in the treatment of male infertility, accurate curative effect, low price, easy to be accepted by patients, and has become the clinical treatment of this disease.
One of the important means. Therefore, it has important clinical guiding significance to summarize and summarize the research on the medication rules of traditional Chinese medicine in the treatment of male infertility. The national patent database collects the strengths of all doctors and brings together a large number of remarkable curative effects.
of traditional Chinese medicine. This research collects the traditional Chinese medicine compound prescriptions for the treatment of male infertility in the national patent database in the past 10 years and uses the traditional Chinese medicine inheritance computing platform V3. Provide a reference for organizing and creating new parties.
Click here to know more about male infertility
1 Material and methods
1.1 Document sources
Log on to the China Patent Publication Bulletin website (http://epub.cnipa.gov.cn), enter the "Advanced Query" interface and select "Invention Announcement" and "Invention Authorization". The search time is from January 2010 to December 2021. As a retrieval method, 238 Chinese herbal medicines for male infertility in the past 10 years were preliminarily obtained by combining the retrieval results.
1.2 Screening Criteria
1.2.1 Inclusion criteria
(1) Chinese medicine compound for the treatment of male infertility;
(2) The patent of Chinese medicine extract for the treatment of male infertility;
(3) Patent items
There is no treatment for male infertility in the project name, but the patent details page states that it can be used to treat male infertility.
1.2.2 Exclusion criteria
(1) Chinese medicine compound is a food patent;
(2) The traditional Chinese medicine compound belongs to the patent of health care products;
(3) Not given in the abstract or description
Compounds made up of specific drugs;
(4) Compounds with the same dosage and composition are entered only once.
1.3 Analysis software
Traditional Chinese Medicine Inheritance Computing Platform V3.0 software, developed by China Traditional Chinese Medicine Science
Provided by the Institute of Traditional Chinese Medicine.
1.4 Standardized treatment of traditional Chinese medicine
Chinese medicine names were standardized with reference to the Pharmacopoeia of the People's Republic of China. The medicinal effect is the same, but the processing method is different, for example, "making aconite" is changed to "aconite"; the medicine is the same, but the name is different, such as "Dayun" is changed to "Cicstanche" and so on.
1.5 Data analysis methods
According to the inclusion and exclusion criteria, 123 TCM compounds that meet the conditions were finally screened out, and the compound drugs were entered into an Excel table
, the input method of double input is adopted. Use the traditional Chinese medicine inheritance computing platform V3.0 software to analyze the drugs in the Excel table, including statistical analysis (frequency of traditional Chinese medicine, medicinal properties, medicinal taste, meridian return, efficacy), association analysis, cluster analysis.
2 results
2.1 Statistics on the frequency of drug use for male infertility
There are 30 drugs with a frequency of use ≥18 times, and the top 6 traditional Chinese medicines are Dodder, Lycium barbarum, Epimedium, Rehmannia glutinosa, Angelica sinensis, and Cistanche.
See Table 1.
2.2 Distribution of drug properties and flavors
The properties of drugs are mainly warm, and flat, cold, hot, and cool. The taste of the medicine is mostly sweet, followed by bitter, acrid, sour, and salty. See Table 2.
2.3 Statistics of drug effect in channels
The kidney and liver are the main target that drugs enter, followed by the spleen, lung, heart, stomach, large intestine, bladder, gallbladder, small intestine, and Sanjiao meridian. see picture 1.
2.4 Statistics of drug efficacy
The 220 traditional Chinese medicines were classified according to their efficacy, involving 20 categories of efficacy categories. The most frequent occurrences were those of tonifying deficiency, followed by those of promoting blood circulation and removing blood stasis, and clearing heat. See Table 3.
Table 3 Statistical distribution of efficacy of patented traditional Chinese medicine compound prescriptions for male infertility
2.5 Statistics of prescription drug combination
Set "Number of Supports" to 15 and "Confidence" to 0.7. Among the top 20 drug pair combinations, "Cuscuta japonica - Lycium barbarum" appeared most frequently. See Table 4. Select "rule analysis" for drug combination analysis, extract 56 association rules, and draw a network diagram of the rules. Combinations with high confidence include "Cuscuta, Chinese yam → wolfberry", "Chuanxiong → Angelica" and so on. See Table 5 and Figure 2.
Table 4 The top 20 drug-pair combinations in the frequency of patented traditional Chinese medicine for male infertility (number of support = 15, confidence = 0.7)
2.6 Clustering statistics of prescription drugs
According to the number of prescriptions and clinical experience, the number of clusters is designed to be "5", and 5 core prescriptions of the traditional Chinese medicine compound patent for male infertility are obtained: "Angelica Sinensis, Rehmannia glutinosa, Dodder, licorice, Astragalus, Chuanxiong", "Rehmannia glutinosa, Astragalus, Dodder, Polygonatum, Epimedium, Scutellaria ", Dodder, Wolfberry, Epimedium, Polygonatum, Plantago, Dandelion, Ginseng, Astragalus, Ganoderma lucidum, Dodder, Epimedium, Atractylodes, Epimedium, Lycium barbarum, Dodder, Raspberry, Cistanche, Morinda officinalis. See Table 6
Yin and Yang Definition in Traditional Chinese Medicine
Table 6 Combination of core clustered prescriptions patented traditional Chinese medicine compound for male infertility (number of clusters = 5)
3 Discussion for male infertility
Male infertility belongs to the categories of "childlessness" and "sterility" in traditional Chinese medicine. The etiology and pathogenesis of this disease are complex, and it is not caused by a single pathogen or the involvement of a single viscera. "Shi Shi Mi Lu, Book Collection, an ancient chiese medical instruction book " states: "There are six diseases in which men cannot have children... What are the dimensions of the six diseases? One essence One is cold, one is weak, one is too much phlegm, one is fire, the other is scarce, and the other is stagnant." It is believed that the cause and pathogenesis of male infertility are no more than the two ends of deficiency and excess, and deficiency has a deficiency of qi and blood, and yin and yang. , there are differences between cold, phlegm, fire, and depression.
The normal operation of male reproductive function depends on the coordination of viscera, meridians, qi, blood, etc. Any disorder in any link, such as visceral disease, meridian stasis, qi and blood deficiency, etc., can lead to male infertility. Based on this study, the etiology and pathogenesis of male infertility can be classified as "deficiency", "blood stasis", "phlegm turbidity", "damp heat", "qi stagnation", etc., and they can be both diseases.
3.1 Analysis of medicinal properties and medicinal taste
"Shen Nong's Materia Medica·Preface" says: "Medicine has five flavors: sour, salty, sweet, bitter, and pungent, as well as cold, hot, warm, and cool."
Statistics on the nature and flavor of all traditional Chinese medicines in 123 compound prescriptions were conducted to treat men. The medicinal properties of infertility medicines are mainly warm, followed by flat and cold; the medicinal tastes are mostly sweet, followed by bitter and acrid.
The kidney is the house of yin and yang, and the place to store the essence. The use of medicine must be careful to avoid damage to yin and yang. ' Collection Secret Records, Miscellaneous Diseases and Sizes of Heshen, Volume 17, which says 'Too much dryness and heat will deplete the true yin.' It can also disturb the relative fire, making the man indulge in indulgence, the essence is thin, and there is no child; it is not allowed to attack too much, too cold and cold will damage the yang, easily block the sperm, and reduce the ability to produce sperm. When using warm and calming medicine to protect the true yang and restrain the true yin. " Chinese Compendium of Materia Medica experts" says: "Bitterness can purify, dryness, and firmness; sweetness can nourish, harmonize, and slow motion; acrid ones can disperse, moisten, and run rampant." The pathogenesis of fertilization disease is mostly based on deficiency and actuality, and the "sweet" flavor can supplement and cure the root; The "pungent" flavor moves Qi and blood.
3.2 Analysis of drug meridian return Meridian return is the positioning concept of the effect of traditional Chinese medicine.
The most frequent are the kidney, liver and spleen meridians. From the theory of the kidney, the ancient Chinese medicine book records "Plain Questions: The Six Sections of Zangxiang Theory": "The kidney is the main function of the sex, the root of the storage, and the essence." The kidney stores the essence and controls reproduction. Only when yin and yang are in harmony can one conceive and have a child. From the perspective of the liver, essence and blood have the same origin, and the treatment of blood should not be neglected when treating essence. Therefore, physicians attach great importance to the simultaneous treatment of liver and kidney. While using wolfberry, cistanche, dodder, dogwood, epimedium and other medicines to invigorate the kidney and replenish essence, they also use white peony root, angelica, donkey-hide gelatin, mulberry and other medicines to nourish liver and blood, or in combination with Bupleurum, Citrus aurantium and other medicines soothe the liver and relieve depression.
In terms of the spleen, the kidney is the foundation of the innate, the spleen is the foundation of the acquired, and the acquired is nourished to nourish the innate. The spleen is full of energy, the qi and blood are biochemically active, and the essence room can be nourished, just as it is said in "Fu Qingzhu Nvke, Nvke Part II, Pregnancy, Abdominal Pain in Pregnancy": "The spleen is acquired, and the spleen is not innate. If the qi that is not acquired cannot be produced, and the kidney is tonified without tonifying the spleen, then how can the essence of the kidney be produced.” In addition, the course of male infertility is relatively long, and applying the method of strengthening the spleen can not only prevent the long-term medication from damaging the spleen and stomach, but also promote the absorption of the medication and improve the curative effect. Therefore, the clinical treatment of male infertility focuses on the kidney and the liver.
3.3 Analysis of drug efficacy
This study shows that there are 20 categories of drug efficacy, with the most frequently used drugs for tonifying deficiency, followed by those for promoting blood circulation and removing blood stasis, and for clearing heat. Among the tonic medicines, yang-tonifying medicines are used most frequently. Experts believe that deficiency of kidney-yang and depletion of vital fire will inevitably lead to the decline of human reproductive function, resulting in oligoasthenozoospermia. Relevant data show that oligoasthenospermia is one of the most important factors leading to male infertility. Varicocele is an important cause of male infertility. Clinically, blood-removing blood stasis method is often used to treat varicocele infertility. Improve the ischemia and hypoxia of testicular tissue, avoid the stay of toxic metabolites, and achieve the purpose of protecting sperm. Reports suggest that chlamydia and mycoplasma genital tract infections can lead to male infertility.
Experts believe that the pathogenesis of reproductive tract infection is infertility caused by exogenous damp heat and poisonous pathogens invading the essence orifices. Studies have shown that most of the heat-clearing and damp-damping herbs have anti-inflammatory, antibacterial, detoxifying, improving local inflammation and reducing tissue damage effects.
To sum up, modern physicians are flexible in the treatment of male infertility, and attach importance to the combination of disease differentiation and syndrome differentiation.
Combined, different treatment methods are used according to the infertility caused by different causes.
3.4 Drug Frequency Analysis
Statistics on the frequency of traditional Chinese medicines used to treat male infertility show that dodder, wolfberry, epimedium, rehmannia glutinosa, angelica, and cistanche are more frequently used. Among them, dodder, wolfberry, rehmannia, and angelica are the core components of Yougui Pills. Pharmacological studies have found that Yougui Pill can affect the changes of hormone levels such as testosterone and estradiol through the hypothalamus-pituitary-gonadal axis, thereby improving the reproductive function of the body.
Epimedium is warm in nature, enters the liver and passes through the kidney, and is mainly used for invigorating the kidney and strengthening yang. Pharmacological studies have shown that icariin can inhibit the oxidative stress injury of human sperm induced by peroxide/ferrous sulfate, activate the ERK1/2 signaling pathway, and promote testicular oxidative stress. Cell proliferation, enhance male reproductive function.
Cistanche deserticola has the reputation of "desert ginseng". It is warm in nature but not hot, good for nourishing but not harsh, and has the power of invigorating the kidney and nourishing essence. Modern pharmacology shows that Cistanche deserticola can increase the level of testosterone, improve the microenvironment of the epididymis, and increase the number of interstitial cells in the testis. , thereby enhancing the spermatogenic function of the testis. It can be seen that high-frequency traditional Chinese medicine may promote the proliferation of testicular cells, improve sperm quality, and regulate sex hormones through the mechanism of "multi-component-multi-target" to achieve clinical effects in the treatment of male infertility.
3.5 Analysis of drug association rules
It is the most commonly used drug pair for the treatment of male infertility. The two medicines are the monarch medicine and the minister medicine of Wuzi Yanzong Pills. In the prescription, wolfberry is used to replenish yin and yang, replenish essence and fill marrow. It is monarch medicine. "Compendium of Materia Medica, Caomu Shang, Lycium barbarum" calls it: "replenishing the essence and qi, and strengthening the vagina". ; Dodder can not only invigorate kidney yang, but also nourish kidney yin, and it is the main medicine. "Materia Medica Huiyan, Caobu, Crescendo, Dodder," says that it is "tonifying but not severe, warm but not dry". Network pharmacology indicated that the dodder-lycium barbarum drug pair might treat male infertility by inhibiting inflammatory response, inhibiting apoptosis and promoting spermatogenesis.
The experimental study found that the dodder-lycium barbarum drug pair can effectively alleviate the spermatogenic dysfunction caused by tripterygium wilfordii polyglycosides. "Dodder, Chinese yam → Lycium barbarum" has the highest confidence in the combination. This is the main drug of Zuogui Pill in "Jingyue Quanshu·Xinfang Bazhen·Buizhen". Chinese yam tonifies the kidney and strengthens the spleen, and treats all kinds of deficiency and damage. The combination of the three medicines is warm but not dry, nourishing but not greasy. Clinical studies have found that Zuogui Pills can improve sperm count, and motility and effectively repair sperm DNA damage. It can be seen from the drug association rules that physicians attach importance to the addition and subtraction of Wuzi Yanzong Pills and Zuogui Pills for the treatment of male infertility.
3.6 Drug Cluster Analysis
Based on the entropy clustering extraction of complex systems, and using K-value clustering algorithm (cluster analysis algorithm for iterative solution), five types of drug core combinations were screened out. Clustering is to classify the included data that are the same or similar in a certain aspect to form new groups.
The first category is based on Cistanche Angelica sinensis, Rehmannia glutinosa, Dodder, Licorice, Astragalus, Chuanxiong, and is derived from Siwu Decoction. infertility;
The second category is based on Rehmannia glutinosa, Astragalus, Dodder, Polygonatum Radix, Epimedium, Cistanche deserticola, and Glycyrrhiza chinensis. All medicines are combined with yin and yang, breath power, and blood, and can be used for men with yin and yang deficiency.
The third category is based on dodder, medlar, epimedium, Polygonatum chinensis, psyllium, and dandelion. It has the effects of invigorating the kidney and nourishing essence, clearing heat and removing dampness. It can be used for male infertility due to kidney deficiency and damp heat. disease;
The fourth category is based on ginseng, astragalus, ganoderma lucidum, dodder, epimedium, and atractylodes, and it is used for male infertility with deficiency of spleen and kidney;
Category 5 is based on Epimedium, Wolfberry, Dodder, Raspberry, Cistanche, Morinda Officinalis, and is derived from Wuzi Yanzong Pill, which is known as "the first party of ancient and modern seeds", and can be used for kidney-yang deficiency. Decaying male infertility.
It can be seen from these five types of clusters that modern doctors focus on invigorating the kidney and nourishing essence when treating male infertility. Governing Law.
4 Conclusion for male infertility
This research uses the traditional Chinese medicine inheritance computing platform V3.0 software to conduct systematic and in-depth data mining of Chinese medicine compound patents for the treatment of male infertility. Medicinal flavor, meridian return, and efficacy; correlation analysis reflects the relatively close and stable Chinese herbal medicine pairs in the compound; cluster analysis objectively reveals the general tendency and the law of prescriptions for male infertility. The combination of various analysis methods is expected to explore the characteristics of traditional Chinese medicine treatment of male infertility, provide a reference for clinical prescriptions and create new prescriptions, and have a certain positive role in promoting the treatment of male infertility by traditional Chinese medicine.
FIND THE BEST BOTANICAL HOLISTIC CARE FOR Male Infertility
Our carefully hand-crafted improved sexual function formula includes the best-researched herbs- cistanche pruducts. Each shows amazing potential for nerve pain management, plus general nerve and neuron support overall.
Includes:
Cistanche herb has the efficacy of regulating the neuroendocrine system and invigorating the kidney and yang. Cistanche has an androgen-like effect and can increase the release of monoamine neurotransmitters in the central nervous system by inhibiting the activity of hypothalamic monoamine oxidase and enhancing gonadal function, thus achieving prevention of Low sexual behavior, the purpose of improving sexual dysfunction. Japanese scientists have also discovered a "life-nourishing factor" from Cistanche, which can increase the proliferation rate of kidney cells six times within 24 hours.
The Key to Improve sexual function：– to improve memory and prevent AD Disease, contains cistanche extract dodder extract, microcrystalline cellulose, magnesium stearate,
It can nourish the kidney and Yang! Cistanche is beneficial to essence and blood, moisturizing the intestines and laxative effects. Cistanche is often used to treat impotence, premature ejaculation, infertility, irregular menstruation, and infertility due to kidney yang deficiency. Therefore, Cistanche is used to invigorate kidney yang deficiency and treat all symptoms of kidney yang deficiency.
shows support for the sexual function system in studies
Effects of Cistanche Kidney Supplement
Inhibit kidney cell apoptosis
Improve kidney cell proliferation
Repair damaged kidney cells
Dilates the blood vessels of the kidneys
To know more knowledge: wallence.suen@wecistanche.com
