Effects Of Cistanche On Morphology And Histochemistry Of Testis And Epididymis in Mice

Wang Dejun, Sheng Shuqing, Liang Hong,

Department of Histology and Embryology, Yangzhou University School of Medicine, Yangzhou

Contact: joanna.jia@wecistanche.com / WhatsApp: 008618081934791

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Abstract

Objective To observe the effects of Cistanche on the morphology and histochemistry of testis and epididymis in mice. Methods Thirty Kunming male mice were selected and randomly divided into medication group I, group II and control group. Cistanche decoction (4g·kg -1·d-1 and 8g·kg -1·d-1) was used to gavage the stomach, and the control group was gavage with normal saline. Three weeks later, the mice were sacrificed to determine the number of sperm in the semen. Morphological indicators such as viability and running speed, as well as the fructose content in seminal plasma. Histochemical observation of testis and epididymis succinate dehydrogenase and non-specific lipase reactions. Results The above-mentioned indexes of the administration group were higher than those of the control group. Conclusion It suggests that Cistanche has the effect of strengthening yang and nourishing the kidney.

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Keywords: Cistanche; mice; testis; epididymis; morphology; histochemistry

Cistanche is listed as the fleshy stem with scales of the plant Cistanche cistanche. It is a blindly valuable tonic, which has the effects of invigorating the kidney, strengthening the yang, strengthening the liver and kidney, and replenishing essence and qi.[1] Cistanche is the most frequently used drug in the prescriptions for invigorating the kidney and strengthening yang in the past. It is also one of the famous tonic drugs, second only to ginseng[1] 2]. In order to further explore the mechanism of action of Cistanche, we observed the morphology and histochemistry of the testis and epididymis of the mice in the medication group and the control group.

In this experiment, the decoction of Cistanche cistanche was used to gavage male mice at 4g/kg and 8g/kg for 2 to 3 weeks. The sperm count and viability of the mice increased significantly, the sperm running speed increased, and the sperm deformity rate was somewhat declined. According to histomorphological observation, the spermatogenic function of the testis in the medication group was enhanced, the microenvironment of the epididymal tube was improved, and the fructose content increased. Histochemical studies have shown that SDH and NSE are strongly positive.

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Spermatogenesis is a complex multi-phase process, which includes three stages: mitosis of spermatogonia, maturation, and division of spermatocytes, and metamorphosis of sperm cells. Each stage has its own special type of metabolism. The division and spermatogenesis of spermatogenic cells at all levels in the seminiferous tubules require adequate nutrients and the regulation of endocrine hormones. Traditional medicine of the motherland believes that Cistanche has a good effect of nourishing the kidney and strengthening yang, and it can exhibit androgen and gonadotropin-like effects to regulate the function of the endocrine gland axis [6]. Androgens are essential hormones in the process of spermatogenesis. The epididymis is the microenvironment for the further maturation, development, and differentiation of sperm. In this process, sperm relies on the synergistic effect of epididymal epithelium. The resting microenvironment. The content of fructose in seminal plasma is an important component reflecting the function of the epididymis. The content of fructose in the medication group was significantly higher than that in the control group. SDH belongs to the succinate oxidase system of enzymes, which are arranged in chains in the mitochondria. SDH is the first enzyme in this system. It exists in all cells of aerobic respiration and is firmly bound to the mitochondrial membrane. It is the most important dehydrogenase. The enzyme is commonly used in histochemical reactions to reflect the condition of the tricarboxylic acid cycle and become a marker enzyme. NSE activity is related to cholesterol hydrolysis and steroid synthesis and is often used to measure the function of testicular stromal cells [7]. The number of drugs with strong positive reactions of SDH and NSE in the testis and epididymis of the medication group was significantly higher than that in the control group, which proved that Cistanche can promote spermatogenesis of the testis and improve the microenvironment of the epididymis. It provides a reliable morphological basis for the mechanism of Cistanche for nourishing the kidney and strengthening the yang.