Can kidney-tonifying herb cistanche anti-osteoporosis?

Contact: Audrey Hu Whatsapp/hp: 0086 13880143964 Email: audrey.hu@wecistanche.com

Osteoporosis is a systemic metabolic bone disease characterized by decreased bone mass and degradation of bone tissue microstructure; resulting in increased bone fragility and prone to fractures. The disease can be seen in different genders and ages, but it is more common in postmenopausal women and elderly men. At present, there is no ideal clinical method for the treatment of osteoporosis. Western medicines usually use vitamin D, calcium, estrogen, calcitonin, and fluoride preparations. Although they have certain effects, they have many side effects and are expensive. Traditional Chinese medicines prevent and treat osteoporosis. Research is getting more and more attention and full of prospects. Traditional Chinese medicine believes that kidney deficiency is the main cause of osteoporosis. Kidney-tonifying therapy has always been used as the main therapy for osteoporosis. Many traditional kidney-tonifying herbs have obvious effects in preventing and treating osteoporosis. Modern pharmacological studies have shown that kidney-tonifying prescriptions can prevent osteoporosis by regulating multiple target gland axes of the hypothalamus-pituitary gland, promoting the proliferation and differentiation of osteoblasts, inhibiting the proliferation and differentiation of osteoclasts, promoting alkaline phosphatase activity, and affecting the secretion of cytokines.

Osteoporosis

Cistanche is a plant that belongs to the genus Cisanche of Oroharwhaceae, also known as desert ginseng and dayun. It is mainly produced in Xinjiang, Inner Mongolia, Gansu, and Ningxia. Chen Yaqiong et al. found that the pharmacological action of Cistanche has the effects of nourishing the kidney and strengthening the bones and muscles, which is closely related to bone metabolism, and has shown certain activity in the regulation of the nervous system and endocrine system, which is similar to the enhancement of osteogenesis by nest hormone; Wei et al. found that Total phenylethanoid glycoside, echinacoside, and betaine in Cistanche all have androgen-like protein synthesis, promote bone matrix increase and calcium and phosphorus deposition; Zeng Jianchun et al. found that Cistanche medicated serum can promote the proliferation of BMSCs and induce BMSCs The role of osteogenic differentiation. At present, the commonly used animals in the study of osteoporosis are rats, goats, etc. The most commonly used method of modeling is to cut out bilateral ovary models of rats (4). Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF), has an important regulatory effect on osteoclastogenesis. M-KOOPG osteoporotic mice are caused by homologous recombination on mouse embryonic stem cells to cause partial deletion of the endogenous OPG gene sequence, and then through the blastocyst cavity injection of embryonic stem cells and mouse breeding work to obtain OPG gene knockout Mice, OPG knockout mice have typical osteoporosis due to excessive osteoclast production; compared to other animal models of secondary osteoporosis caused by various other inducing factors, they have a stable situation of systemic osteoporosis. The animal itself is not subject to external intervention. It is a good animal model of primary osteoporosis and an ideal animal model for studying osteoporosis at the overall animal level. In this study, the M-KOOPG osteoporosis mouse model successfully introduced by the Xinjiang Medical Animal Model Research Key Laboratory was used to observe the effect of different doses of Cistanche extract on interleukin 1 (Ⅱ4) and tumor necrosis in the bone tissue of M-KOOPG mice. The location and expression of factor-α (TNF-a), to explore the anti-osteoporosis mechanism of Cistanche.

Chinese medicinal herb cistanche-Anti-osteoporosis

Result

Influence on Femur Histopathology According to conventional calcium pathological slices, the bone cortex of the model group is thin, the number of trabeculae is sparse, the number of bone marrow in the bone marrow cavity is also less, the blank area is larger, and the bone marrow cavity is therefore relatively open. Cistanche extract high-dose and low-dose group, Fushan

Compared with the model group, the trabecular bone width, number and cortical bone of the US group increased slightly, but the improvement was not significant; after 60 days of the administration, the trabecular bone width and number of the Cistanche extract high and low dose groups and the Fushanmei group increased. Compared with the model group, the bone trabecula is more widely distributed in the high-dose Cistanche extract group, and the bone microenvironment is significantly improved compared with the model group; The distribution, number, width of bone trabecula and bone marrow cells in the bone marrow cavity in the low-dose liquid group were not as good as those in the high-dose group.

Effect on the expression of IL-1β and TNF-α in femoral tissues IL-1β and TNF-α are similarly distributed in the bone trabecular bone and bone marrow cavity of M-KOOPG mice, mainly located in the osteoblasts around the trabecular bone, In the cytoplasm of osteoclasts and bone marrow stromal cells, the positive expression area was brownish yellow, and the model group showed strong positive expression. Thirty days after the administration, IL-1β and TNF-α were present in the cytoplasm of osteoblasts, osteoclasts, and bone marrow stromal cells on the surface of bone trabecula in the high and low dose groups of Cistanche extract, the Fushanmei group, and the model group. It is obviously positively expressed, and IL-1β and TNF-α are also strongly expressed in the cytoplasm and nucleus of the articular cartilage migration with chondrocytes in this group. After 60 days of the administration, the number of IL-1β and TNF-α positive signal cells in the trabecular bone periphery and the bone marrow cavity in the high and low dose groups of Cistanche extract and the Fushanmei group were significantly reduced, and the staining was lighter than that in the model group. . See Figures 2 and 3. After 30 days of the administration, the positive expression of IL-1β and TNF-α in the high-dose, low-dose groups, and Fosamax group of Cistanche extract was reduced, and there was no statistical difference compared with the model group; after 60 days of the administration, Cistanche was extracted Compared with the model group, the number of IL-1β and TNF-α positively expressed cells in the high and low-dose liquid group, the Fushanmei group and the model group was significantly reduced, the difference was statistically significant (P <0.05), and the Cistanche extract was high, Low-dose group and Fushanmei group, the positive expression of IL-1β, TNF-α is not statistically different

Cistanche extract anti-osteoporosis

Discuss

The pathogenesis of bis is mainly caused by the imbalance of bone resorption and bone formation in the process of bone remodeling caused by multiple factors such as environment and genetics. Disorders of bone resorption and formation involving signal molecules. A variety of cytokines in the bone microenvironment are also involved, especially IIL4 and TNF-α have a strong effect on it. Cytokines such as IILA, IIL-6, TNFe directly or indirectly act on OC precursors; induce them to differentiate into mature OC, promote bone resorption, and lead to osteoporosis. ⅡLA is an inflammatory cytokine that promotes bone resorption. It is related to bone destruction in b, rheumatoid arthritis, and periodontitis; it can directly induce OC monocyte precursor cell fusion, and it can be affected by nuclear factor-B. Body activating factor ligand (RANKL) promotes osteoclast differentiation; promotes bone resorption. Studies have shown that TNF-α can directly induce osteoclast differentiation and stimulate its activity to play an important role in bone metabolism. It can also regulate the activation of osteoclasts in a paracrine form and stimulate bone by affecting the OPCG/RANKL/RANK signaling system. Absorption confirms that TNF-a participates in the bone metabolism process in a variety of ways; it is also a powerful cytokine that promotes bone resorption.

Traditional Chinese medicine believes that the kidney governs bones and produces marrow, and recognizes that the "kidney" and bone have a close internal connection, that is, the so-called "kidney stores essence, essence produces marrow; marrow is hidden in bones; swimming nourishes bones." Studies have shown that Chinese medicine for invigorating the kidney may increase the expression of VDR mRNA by activating the expression of Smad3 mRNA, thereby promoting bone formation: Chinese medicine for invigorating the kidney can up-regulate the expression of the Smad gene in the bone marrow microenvironment, which may be one of the molecular mechanisms of its effect on promoting bone formation and repairing bone damage. One 04. Bian Qin et al. Observed the effects of the effective ingredients of the three representative traditional Chinese medicines epimedium, psoralen, and Ligustrum lucidum on icariin, psoralen, and oleanolic acid in 3 months of ovariectomized rat bone marrow mesenchymal stem cells (BMSCs). It is believed that kidney-tonifying Chinese medicine may promote the osteogenic differentiation of BMSCs in terms of increasing the extracellular matrix of BMSCs, promoting growth factor-related signal pathways, and increasing protein synthesis, and ultimately achieve the therapeutic effect of treating osteoporosis. The exact mechanism remains to be studied in depth. Modern pharmacological studies have confirmed that the pharmacological effects of Cistanche have the effects of invigorating the kidneys and strengthening the bones and muscles; it has a regulatory effect on the nervous system and endocrine system and is closely related to bone metabolism. Fushanmei belongs to the bisphosphonates, which mainly achieves the anti-osteoporosis effect by inhibiting bone resorption mediated by osteoclasts. It has been the first-line drug for the prevention and treatment of osteoporosis in recent years, and its good clinical efficacy has been affirmed at home and abroad. This study showed that after 60 days of continuous administration, HE staining of mice in the high and low dose groups of Cistanche extract, and the Fuchsia group showed that the width of bone trabecula increased, the number increased, and the fracture decreased; the bone cortex thickened, which was significant compared with the model group. Difference; immunohistochemical index L48. The number of TNF-α positive expressing cells is significantly different from the model group; Ⅱ4A; TNFa shows the cytoplasmic expression of osteoblasts, osteoclasts, and bone marrow stromal cells on the surface of trabecular bone It is reduced to varying degrees, which suggests that Ⅱ.-β, TNEFa acts on bone formation and bone resorption through autocrine and paracrine forms; this is consistent with the domestic Fan Hongbin et al. (4). There was no significant difference between the groups, suggesting that the Cistanche extract and Fushanmei have similar anti-osteoporosis effects.

The study also found that the anti-osteoporosis effect of Cistanche deserticola extract existed time-dependently; after the mice were fed the drug for 30 days, the number of bone trabecula and the number of IL-β and TNF-positive cells were not statistically different among the four groups. Significance: After 60 days of the administration, there were statistically significant differences in the number of trabecular bone and IIL-18; TNF-a positive expression cells in the experimental group, control group, and model group. Pathological observation also showed that after 60 days of administration, Cistanche extract can improve the bone trabecular structure, and the high-dose group improved significantly. Therefore, Rou Ai Rong can improve the pathological changes of osteoporotic mice. It shows that the treatment time of Cistanche extract should be at least one month, and this continuous long-term treatment is also consistent with Fushanmei. Xinjiang Rouhuan Rong has abundant resources in Xinjiang. In the long-term clinical application, there has been no adverse reaction of Xinjiang Rouhuanrong. It has a broad prospect for medicinal use. This experiment provides a theory for the future clinical application of Xinjiang Cistanche extract Basis and experimental basis.

Cistanche tonify kidney so to boost the production of bone marrow

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