The Germplasm Collection And Quality Evaluation Of Cistanche Tubulosa From Xinjiang Ⅲ
Jul 17, 2024
3. Comparison of fresh weight, dry weight and dry-to-dry ratio of different germplasms of C. tubulosa
The fresh weight and dry weight of C. tubulosa were measured, as well as the weight of the samples before and after treatment. The dry-to-dry ratio of C. tubulosa at different sampling points in the two sampling seasons was calculated (dry-to-dry ratio = dry weight/fresh weight*100%), and variance analysis was performed. The results of dry weight and fresh weight are shown in Tables 4 and 5, and the results of dry-to-dry ratio are shown in Tables 6 and 7.

HEDIAN PRODUCED CISTANCHE FOR SALE
| Region | Code | Source | Fresh Weight (g) | Coefficient of Variation | Dry Weight (g) | Coefficient of Variation |
|---|---|---|---|---|---|---|
| Cangxi | CL11-CL12 | Cultivated | 396.81±168.53bcd | 0.42 | 93.69±42.33bc | 0.45 |
| Hedian | HT11-HT13 | Cultivated | 437.65±151.67bcd | 0.35 | 99.57±39.54bcd | 0.40 |
| Minfeng | MF11 | Wild | 421.16±294.95bc | 0.70 | 123.42±53.16ab | 0.43 |
| MF12 | Wild | 495.51±401.53bc | 0.81 | 135.26±106.44ab | 0.79 | |
| MF14 | Wild | 320.74±168.91bcd | 0.53 | 86.39±45.90bc | 0.53 | |
| Mojiang | MY11 | Cultivated | 595.96±277.28ab | 0.47 | 121.52±58.00bc | 0.48 |
| Qianmei | QM11 | Cultivated | 673.63±123.30a | 0.18 | 137.97±27.23a | 0.20 |
| QM12 | Wild | 308.85±195.12bcd | 0.63 | 89.53±56.00ab | 0.62 | |
| Yutian | YT11 | Cultivated | 253.23±59.67cd | 0.24 | 92.33±42.33bc | 0.45 |
| YT12 | Cultivated | 343.70±288.70bc | 0.84 | 92.33±53.90bc | 0.58 | |
| YT14 | Cultivated | 690.43±130.15a | 0.19 | 132.34±25.02ab | 0.19 | |
| YT15 | Cultivated | 395.19±254.10bc | 0.64 | 96.91±61.51bc | 0.63 | |
| YT16 | Cultivated | 452.07±202.24bc | 0.45 | 128.93±56.00ab | 0.43 |
Note: Different letters indicate significant differences at the 0.05 level (double-sided) (P<0.05).
| Region | Code | Source | Fresh Weight (g) | Coefficient of Variation | Dry Weight (g) | Coefficient of Variation |
|---|---|---|---|---|---|---|
| Cangxi | CL21 | Cultivated | 400.25±235.14ab | 0.59 | 84.01±63.42bc | 0.75 |
| Hedian | HT21-HT22 | Cultivated | 527.65±285.54ab | 0.54 | 83.37±45.19bc | 0.54 |
| HT23 | Cultivated | 603.95±247.17ab | 0.41 | 116.61±66.29abc | 0.57 | |
| Minfeng | MF21 | Wild | 421.50±219.93ab | 0.52 | 109.58±53.70abc | 0.49 |
| MF22 | Wild | 414.37±217.38ab | 0.52 | 114.15±54.50abc | 0.48 | |
| MF23 | Wild | 523.34±307.22ab | 0.59 | 156.43±120.51a | 0.77 | |
| Mojiang | MY21 | Cultivated | 454.63±363.72ab | 0.80 | 102.58±70.70abc | 0.68 |
| Yutian | YT21 | Cultivated | 371.23±199.69ab | 0.54 | 52.30±34.26c | 0.66 |
| YT23 | Cultivated | 307.62±216.50b | 0.70 | 57.40±42.74bc | 0.74 | |
| YT24 | Cultivated | 709.46±555.73a | 0.78 | 134.74±74.66ab | 0.55 |
| Region | Code | Source | Starch Content (%) | Coefficient of Variation |
|---|---|---|---|---|
| Cangxi | CL11-CL12 | Cultivated | 23.50±3.43c | 0.15 |
| Hedian | HT11-HT13 | Cultivated | 23.05±5.56c | 0.24 |
| Minfeng | MF11 | Wild | 33.68±8.14b | 0.24 |
| MF12 | Wild | 29.07±4.54bc | 0.16 | |
| MF14 | Wild | 26.21±9.20bc | 0.35 | |
| Mojiang | MY11 | Cultivated | 20.39±2.94c | 0.14 |
| Qianmei | QM11 | Cultivated | 33.55±12.06b | 0.36 |
| QM12 | Wild | 28.78±8.71bc | 0.30 | |
| Yutian | YT11 | Cultivated | 46.68±5.02a | 0.11 |
| YT12 | Cultivated | 29.13±6.82bc | 0.23 | |
| YT14 | Cultivated | 24.43±2.00c | 0.09 | |
| YT15 | Cultivated | 29.31±8.59bc | 0.29 | |
| YT16 | Cultivated | 28.91±3.55bc | 0.12 |
Note: Different letters indicate significant differences at the 0.05 level (double-sided) (P<0.05).
The fresh weight and dry weight of different sampling points in the autumn of 2015 and the spring of 2016 were compared respectively, and the samples from the sampling points with more consistent GPS data in the two years were selected to compare the fresh weight and dry weight. Analysis of variance was performed using SPSS software, and different letters indicate significant differences at the 0.05 level (two-sided).

Sampling point
Figure 5 Comparison of fresh weights at different sampling points in the autumn of 2015 Note: Different letters indicate significant differences (P<0.05) at the 0.05 level (both sides). The fresh weights of different sampling points in the autumn of 2015 are shown in Figure 5, the same rectangle The sampling points in the box are from the same county or city. There are significant differences in the fresh weight of different sampling points. The fresh weight value of the YT 14 sampling point in Yutian County is the largest, and the two sampling points in Mo County are the smallest. Fresh weight has some relationship with some factors such as host status and soil status. Autumn 2015

The dry weight of different sampling points in each season is shown in Figure 6. The difference between different sampling points is not significant. The fresh weight of YT 14 sampling point in Yutian County is the heaviest, so its dry weight value is also the largest. The coefficients of variation of fresh weight and dry weight of samples in the autumn of 2015 were not large, indicating that there was not much weight difference between samples at each sampling point.

Sampling point
Figure 6 Comparison of dry weight at different sampling points in the autumn of 2015
Note: Different letters indicate significant differences at the 0.05 level (both sides) (P<0.05)

The fresh weight of different sampling points in the spring of 2016 is shown in Figure 7. The difference between different sampling points is not significant. The fresh weight value of sampling point YT24 in Yutian County is the largest, while the fresh weight value of YT23 is the smallest. The sampling points within the same rectangular frame are from the same county or city. Fresh weight has some relationship with some factors such as host status and soil status. The dry weight of different sampling points in the spring of 2016 is shown in Figure 8. There are significant differences between different sampling points. The fresh weight of sampling point YT24 in Yutian County is the heaviest, but its dry weight value is not as large as that of MF23 sampling point in Minfeng County. Moisture content is lower. The coefficient of variation of the fresh weight and dry weight of samples in the spring of 2016 was not large, indicating that there was not much difference between each sample at each sampling point.
Sampling point

Figure 7 Comparison of fresh weight at different sampling points in the spring of 2016 Note: Different letters indicate significant differences at the 0.05 level (both sides) (P<0.05)

Sampling point
Figure 8 Comparison of dry weight at different sampling points in the spring of 2016 Note: Different letters indicate significant differences at the 0.05 level (both sides) (P<0.05)

3.2 Analysis of the drying rate of tuberous meat from different germplasm
Measure the fresh weight and dry weight of the pork belly, the weight before and after treatment, and calculate the dryness rate of the pork belly at different sampling points in the two sampling seasons (dry rate = dry weight/fresh weight * 100%). SPSS software was used for analysis of variance, and different letters indicate significant differences at the 0.05 level (two-sided).
Comparing the dryness rate of tuber flower meat from different sampling points in the autumn of 2015, the YT11 sampling point has the highest dryness rate, that is, the lowest moisture content. This may be related to the fact that it is a wild sampling point. Analysis of variance was performed through SPSS software. Different letters indicate significant differences at the 0.05 level (two-sided). There were significant differences in the dryness rates of different sampling points in the autumn of 2015.

Sampling point
Figure 9 Comparison of dryness rates of T. tubulosa from different sampling points in the autumn of 2015
Note: Different letters indicate significant differences at the 0.05 level (both sides) (P<0.05 sampling point

Figure 10 Comparison of the dryness rate of tube flower meat Congrong at different sampling points in the spring of 2016
Note: Different letters indicate significant differences at the 0.05 level (both sides) (P<0.05
Comparing the dryness rate of tuber meat from Rong in the spring of 2016, the sample from Minfeng County has the highest dryness rate, and the dryness rate also explains the moisture content of the sample. Analysis of variance was performed using SPSS software, and different letters indicate significant differences at the 0.05 level (two-sided). The dryness rate of the samples in Hotan City is significantly different from that in other production areas. The dryness rate of the Hotan sampling point is the lowest, which may be related to the sufficient soil moisture content in the cultivation area.
Select the samples with similar GPS data of the two-year sampling points from Rongcheng, and use SPSS software to perform a T test on the dryness rates in different seasons. It is found that there is a significant difference between the two. The dryness rate is higher than that in the spring of 2016, that is, the moisture content of spring samples is higher. This may be related to changes in soil moisture content, because only one year of data is examined, and samples will continue to be collected for verification in the future.

Origin
Figure 11 Comparison of dryness rates of tube flower pork Congrong samples in the autumn of 2015 and the spring of 2016
Section 3 This Chapter
This study collected a total of 285 accessions of cistanche tubulosa from 6 counties and cities in southern Xinjiang, including 180 samples collected from 17 sampling points in autumn and 105 samples collected from 12 sampling points in spring; including wild samples 133 samples and 152 cultivated samples; after that, all the samples of cistanche tubulosa were morphologically measured, weighed and processed.
The main results are as follows: The dryness rate of tube flower meat in autumn is higher than that in spring, that is, the moisture content of spring samples is higher.
Comparison of plant heights of cistanche tubulosa from different germplasms. The plant heights of cistanche tubulosa from different germplasm vary greatly in different seasons and at different sampling points in autumn. However, there is no significant difference in the plant height of cistanche tubulosa at different sampling points in spring, while the differences between samples in different sampling seasons are extremely significant. The plant height of C. tubulosa in the spring of 2016 was significantly higher than that of the autumn 2015 samples. It is speculated that this is because after the winter, the flower C. tubulosa gradually transitioned from vegetative growth to reproductive growth, and the inflorescence end was significantly elongated.






