Chemical Components Identification Of Cistanche Deserticola Using The X500R QTOF System
Mar 02, 2022
Contact: Audrey Hu Whatsapp/hp: 0086 13880143964 Email: audrey.hu@wecistanche.com
Cistanche Deserticola was first recorded in the “Shen Nong Materia Medica”; it is also known as “Dayun,” “Rousong Rong,” and “Zong rong.” As a traditional herbal medicine, it has the properties of nourishing the kidney yang, improving blood flow, acting as a laxative, immune stimulation, and other effects[1]. In1983, the Japanese scholar H. Kobayashi and others began to study the chemical composition of Cistanche Deserticola[2], and since then it has become a popular topic in Chinese medicine research that has generated great interest both domestically and abroad over the last 30 years. Cistanche Deserticola belongs to the class of plants containing echinacoside, acteoside(verbascoside), flavonoids, phenolic glycosides, iridoids, and lignans and their glycosides.
Quadrupole time-of-flight (QTOF) mass spectrometry is a sensitive and specific tool for the identification of Chinese medicine components that have gradually become indispensable to research. This technology has overcome traditional technical challenges with retrospective analyses of single injections that permit extraction of important data and the most comprehensive acquisition of sample information. Using exact mass and high-resolution TOF-MS and TOF-MS/MS data allows for simultaneous, highly specific targeted and non-targeted qualitative analysis. However, the complexity of the instrument operation and software use has vastly limited the spread and development of this technology. Here we introduce a new QTOF system that uses a revolutionary new N-type geometry-based TOF path, intuitive software, and accurate molecular weight techniques that are easier to use in Chinese medicine component identification.
Main effective ingredients of cistanche deserticola: Echinacoside
The benefits of this method are as follows:
1. The X500R uses the durable, industry-approved Turbo VTM ion source and air curtain gas interface design, which has a strong anti-contamination feature;
2. SCIEX OS Software has integrated acquisition, processing, and reporting functions on a single platform; the interface is intuitive, easy to master, and has a one-touch auto-adjust correction to ensure that analysts with any degree of expertise can obtain high quality, reliable data;
3. Using SCIEX OS Software to process data to identify Chinese medicine components are simple and permit rapid extraction of useful information, thus improving efficacy;
4. It derives more accurate and reliable identification results from Chinese medicine libraries containing MS/MS spectra;
5. High-resolution MS/MS Chinese medicine databases are based on the “Chinese pharmacopeia” Part 1 TCM active ingredients; including component references in the pharmacopeia and active ingredients in the herbs, there are nearly 900 compounds
Main effective ingredients of cistanche deserticola: Acteoside
Experimental Process:
1. Using TOF-MS-IDA MS/MS mode, inject a sample and simultaneously obtain primary precursor ions and corresponding secondary spectra;
2. Using SCIEX OS Software targeted screening, confirmation of target compounds, and secondary spectra along with a screening of Chinese medicine standards and matching methods can increase accuracy and work productivity.
3. SCIEX OS Software’s non-targeted identification workflow uses library searches and complete unknown searches in ChemSpider to verify results, ensuring more components are identified with a simpler workflow.
Effects of cistanche deserticola: IMPROVE IMMUNITY
