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Menopausal hypertension is a blood pressure fluctuation caused by endocrine and metabolic disorders of the body accompanied by autonomic nervous system dysfunction. Nearly 80% of women will experience this symptom. Moreover, blood pressure fluctuations at this stage are easily interfered with by external factors such as emotions and sleep, leading to A variability of blood pressure is large, and it is usually lowered

The control of pressure medication is not ideal, and long-term use may cause varying degrees of adverse reactions. Modern research shows that abnormal fluctuations in blood pressure during menopause may be significantly related to changes in endocrine hormone levels [1]. Clinical treatment mainly uses estrogen replacement therapy for intervention, and this method is not suitable for patients with hormone contraindications, such as liver and kidney dysfunction, Breast cancer, diabetes, etc., with this

At the same time, it will also increase the risk of cardiovascular and cerebrovascular diseases and reproductive system side effects in patients [2]; compared with the treatment of this disease in traditional Chinese medicine, it is more advantageous. The syndrome differentiation prescription of traditional Chinese medicine can not only control blood pressure in different ranges The regulation can also improve the patient's sleep status and alleviate the patient's clinical symptoms. The clinical treatment is well received by patients for its precise curative effect and few toxic and side effects. Cistanche deserticola is a parasitic plant of the Orobanchiaceae family. It is a "medicine and food" product with high nutritional value and unique medical and healthcare functions. Among them, polysaccharide (cistanche deserticola polysaccharides, CDPS) is its main biologically active component. There are studies Have shown that preparations made from Cistanche deserticola can improve the clinical symptoms of menopausal syndrome [3], and Cistanche deserticola has the effect of stimulating follicle development and retinogenesis in female mice with kidney deficiency, showing that Cistanche deserticola can regulate endocrine function of female mice with kidney deficiency. [4], but there has not been an in-depth study on the regulatory mechanism of Cistanche deserticola polysaccharide. Based on this, this study replicated the animal model of menopausal hypertension and dryness syndrome based on the clinical indications of menopausal women and gave Cistanche deserticola polysaccharide for intervention treatment. To investigate the regulatory mechanism of Cistanche deserticola polysaccharide on the endocrine system of menopausal patients.

HOW LONG DOES IT TAKE FOR CISTANCHE TO WORK?

1 Materials and methods

1. 1 animal

65 healthy female SPF grade SHR rats, body weight (200. 0 ± 15. 0) g, were purchased from Henan Experimental Animal Center, license number SCXK (Yu) 2014-0009. The rats were fed ordinary feed, had free access to water, and were free from noise and other environmental factors.

1. 2 Test drugs and reagents

500 g of Cistanche deserticola decoction pieces (purchased from Zhang Zhongjing Pharmacy, Origin: Inner Mongolia, batch number 20121025); other reagents were of analytical grade. Kuntai Capsule (Guiyang Xintian Pharmaceutical Co., Ltd., batch number 140507); endocrine indicators [estradiol (E2), folliclestimulating hormone (FSH), luteinizing hormone (LH) )] Elisa detection kit was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; neurotransmitters [norepinephrine (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), neuropeptide Y (neuropettide Y, NPY), 5-hydroxyindoleacetic acid (5-HIAA)] Elisa detection kit was purchased from Beijing Sizhengbai Biotechnology Co., Ltd.

1. 3 Instruments and equipment

Ultrasonic cleaner (KQ - 300VDV, Kunshan Ultrasonic Instrument Co., Ltd.); Shimadzu 2010 UV-visible spectrophotometer (Japan); ACCESS fully automatic chemiluminescence immunoanalyzer (BECKMAN COULTER, USA); AB204 - S-type Mei Teller electronic balance (Beijing Mettler-Toledo Co., Ltd.); animal non-invasive blood pressure measuring instrument (BP-100A type, Taimeng Technology Co., Ltd.).

1. 4 methods

1. 4． 1 Preparation of Cistanche deserticola polysaccharide

Take 500 g of Cistanche deserticola pieces, add 10 times the amount of water, soak for 30 minutes, heat and extract 2 times, 1. 5 hours, combine the decoction (brown-red), concentrate to 2: 1 (volume to mass ratio), centrifuge (3000 r/min) for 15 minutes, take the supernatant, add 3 times the amount of 95% ethanol to precipitate, refrigerate and freeze Let stand for 24 hours, centrifuge to separate the precipitate, wash the precipitate with absolute ethanol, acetone, and diethyl ether in sequence, dry the washed precipitate in vacuum, and use the anthrone-sulfuric acid method to determine the content of Cistanche deserticola polysaccharide to obtain the crude Cistanche deserticola polysaccharide (ash Brownish red, yield 10.13%).

1. 4． 2 Grouping and administration

All rats were reared in separate cages after purchase, and were divided into groups after 1 week of adaptation. 3 SHR rats with large body weight deviations were eliminated, and the remaining 62 rats were divided into 5 groups according to the random number table method, namely the SHR group and the sham operation group. , model group (menopausal hypertensive dryness syndrome model), positive group (Kuntai capsule, 1. 08g/kg), high-dose Cistanche deserticola group (equivalent to 11.84 g crude drug dosage of Cistanche deserticola, 1. 2 g/kg), low-dose group Dosage group (equivalent to 5.92 g of raw Cistanche deserticola, 0.6 g/kg); the rat dose was converted according to the formula of "Equivalent Dose Conversion between Animals and Animals and Humans in Pharmacological Experiments", 2015 edition of "China According to the Pharmacopoeia, the dosage of Cistanche deserticola is 6 to 10 g/d, and 1 g of crude Cistanche deserticola polysaccharide is equivalent to a dosage of 9. 87 g. Before administration, take an appropriate amount of Cistanche deserticola polysaccharide in sterile physiological saline and prepare it to 0. 1 g/mL Cistanche deserticola polysaccharide solution, the content of the positive group was taken to prepare a concentration of 0. 1 g/mL aqueous solution, the dosage of each dose is calculated according to the body weight, and the normal control group and the model group are given equal doses of physiological saline. In addition to Cistanche deserticola

Except for the two dose groups of polysaccharide, which had 11 rats each, the remaining three groups all had 10 rats. Animals in each group were administered drugs continuously for 4 weeks, and changes in animal behavioral characteristics during drug intervention were observed and recorded.

1. 4． 3 Replication of animal models

Refer to the literature method to replicate the menopausal hypertensive dryness syndrome rat model [5-6]. Surgically remove both ovaries and replicate the menopausal hypertensive rats. One week after the model was successfully replicated, special feed was given daily (feed formula: Ordinary rat food + 0.5% cumin + 0.5% spice

Powder + 10% animal fat + 2. 5% fried peanut kernels + 2. 5% cooked sesame) 100 g, drinking water 200 mL, keeping the temperature and relative humidity of the cage constant, and continuously modeling for 3 weeks to replicate menopausal hypertensive dryness syndrome rats. During the model replication, the biological characteristics of the animals were closely observed and recorded in time.

1. 4． 4. Detection of indicators

After the experiment, an animal non-invasive blood pressure measuring instrument was used to detect the systolic blood pressure of the rat tail artery before and after the experiment (measured three times and averaged). Under intraperitoneal anesthesia with 10% chloral hydrate solution (35 mg/kg), about 5 mL of blood was taken from the abdominal aorta, centrifuged at 3 000 r/min for 15 min, the serum was separated into Eppendorf tubes, and frozen at -20°C until tested. Serum endocrine indicators (E2, FSH, LH) were measured using radioimmunoassay. After blood collection, the rats were quickly decapitated and their brains were removed, and the levels of hypothalamic neurotransmitters (5-HT, NE, DA, NPY, 5-HIAA) were measured using chemical fluorescence method.

1. 4． 5 Statistical processing

Using SPSS17. 0 The data processing system performs statistical analysis on the experimental data. First, the experimental data is subjected to a normality test (KS test). Data that conform to the normal distribution and have equal variances are compared between multiple groups using the one-factor analysis of variance method; those that do not conform to the normal distribution The Chi-square test was used for the state distribution data, and P < 0 was used for all statistical comparisons. 05 means the difference is statistically significant.