Effects Of Different Extracts Of Cistanche On Improving The Sexual Performance Of Rats With Kidney-yang Deficiency

Wang Qixin, Chen Zehua, Luo Hujie, Lu Wenji. , Li Chun, Tu Pengfei

1. Modern Research Center of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029;

2. No Limit (China) Co., Ltd., Guangzhou 510663;

3. Beijing University of Traditional Chinese Medicine School of Traditional Chinese Medicine, Beijing 100029

Contact: joanna.jia@wecistanche.com / WhatsApp: 008618081934791

Abstract

Objective

To observe the effects of different extracts of Cistanche on the sexual ability of rats with kidney-yang deficiency model induced by hydrocortisone. Methods: 50 male SD rats were randomly divided into normal group, Yang deficiency model group, Cistanche cistanche total oligosaccharide group, Cistanche cistanche total polysaccharide group, and Cistanche phenyl ethanol total glycoside group. The animal model of kidney-yang deficiency was prepared by intramuscular injection of hydrocortisone for 14 consecutive days. The drug group was given Cistanche cistanche total oligosaccharide 103.82mg·kg~ and Cistanche cistanche total polysaccharide 115.64mg·kg~ according to 2.3g·kg of crude drug of Cistanche. Cistanche phenethyl total glycosides 201.48mg·kg aqueous solution, once a day. 14 days later, the erectile latency, mating ability indicators (including capture latency, capture frequency, ejaculation latency, ejaculation frequency, capture rate, ejaculation rate, etc.) of each group of rats were measured, wet weight of organs, seminal berry sugar content, and plasma cyclophosphamide Adenosine (cAMP)/CGMP (cGMP) value, serum hormone testosterone (T), estradiol (E,), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and gonadotropin release Hormone (GnRH) level, observe the pathological changes of the testis. Results: Different extracts of Cistanche had effects on erectile function, mating ability, wet weight of organs and sugar content of sperm berry, plasma cAMP/cGMP value, serum hormone level, and pathological changes of testis in rats with kidney-yang deficiency. Among them, the extract of Cistanche phenethyl alcohol total glycosides can significantly shorten the erectile latency (P<0.05) and capture latency (P<0.01) of rats, significantly increasing the number of captures (P<0.01), and testis (P<0.05). 0.05), seminal vesicle gland (P<0.01) wet weight and refined berry sugar content (P<0.05), significantly increased plasma cAMP/cGMP value (P<0.01) and serum T (P< 0.01), FHS (P<0.01), GnRH (P<0.05) levels, significantly reduced serum E, (P<0.05), LH (P<0.05) levels, and significantly improved testes Pathological form. Conclusion: Different extraction parts of Cistanche can be induced by regulating hydrocortisone. The hormone levels of rats with kidney-yang deficiency improve their performance to different degrees. Among them, the total phenethyl alcohol glycosides can significantly improve the related indexes of the rats with kidney-yang deficiency.

Keywords: Cistanche; Kidney Yang deficiency; Performance; Phenylethanol glycosides; Oligosaccharides; Polysaccharides

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Deficiency of kidney yang is caused by the weakening of the kidney yang, insufficient life fire, endogenous yin and cold and soreness and pain in the waist and knees, and men’s hyposexuality, premature ejaculation, and other symptoms of deficiency and cold... Its typical clinical manifestations are hypersexual function. Modern studies have shown that patients with kidney-yang deficiency generally suffer from low gonadal function or reserve function. Traditional Chinese medicine for invigorating the kidney-yang can regulate the function of the hypothalamus-pituitary-gonad (HPG) axis in patients with kidney-yang deficiency, thereby achieving invigorating kidney and strengthening yang, and improving sex. The effect of the ability. Among them, Cistanche cistanche is the most frequently used kidney-yang medicine because of its functions of nourishing kidney-yang, nourishing essence, and blood. It is commonly used for syndromes of insufficient kidney-yang, deficiency of essence and blood, impotence, infertility, and soreness of the waist and knees. Studies have shown that Cistanche Decoction can significantly increase the body mass of mice with kidney yang deficiency, prolong the cold tolerance time of mice with kidney yang deficiency, and suggest that it has the effect of warming and replenishing kidney yang. In addition, clinical studies have shown that Cistanche can regulate the HPG axis function, promote the release of related transmitters and hormones in the body, and improve the performance of patients with kidney-yang deficiency. Therefore, the research on the effective substance basis and the mechanism of action of Cistanche has become a key link in the development of the drug that needs to be solved urgently. However, the current related research reports, especially the research on the substance basis of the action of Cistanche, are relatively small. In this experiment, we established a rat kidney-yang deficiency model induced by hydrocortisone to compare the effect levels of different extracts of Cistanche for nourishing the kidney and strengthening the yang. At the same time, it initially explores its potential mechanism and features, which will reveal the material basis of Cistanche and the subsequent drug development. . 96 · Develop and provide experimental basis.

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Discuss

The kidney is one of the most important viscera of the human body, and Chinese medicine calls it "the foundation of the innate." The vitality of the kidney is up and down, which is related to the reproduction and growth of human beings. Deficiency of the essence and qi in the kidney, and insufficient kidney yang will inevitably affect the reproductive function of the person, and symptoms of impotence, premature ejaculation, and other sexual dysfunction will appear.

The mating experiment is used as the main evaluation indicator for sexual function. By measuring the rat’s capture latency, the number of captures, the latency of ejaculation, the number of ejaculations, the capture rate, and the rate of ejaculation, it can be determined whether the test substance has a good effect on the sex function. The erectile function of the penis is also an important indicator of the evaluation of sexual function. The erection of the penis is caused by the congestion of the spongy body of the penis. The measurement of the erection latency can reflect the reaction speed of the animal's penis to the stimulus. The results of this experiment show that each extracted part of Cistanche has the effect of improving erectile function and mating ability of rats. The total phenethyl alcohol glycosides can not only shorten the erectile latency and capture latency of rats with kidney yang deficiency but also increase the capture latency significantly.

In order to further study the effect of each extract on the morphology and function of the gonads and sexual organs, this experiment selected the testes, epididymis, and seminal vesicles of each group of rats to determine the wet weight of the testis, while observing the pathological changes of the testis. In addition, the seminal vesicle glands secrete fructose to provide the main carbohydrate substance for sperm, and its content is an important indicator for judging sperm motility and fertility. The results showed that different extraction sites of Cistanche can increase the wet weight of testis, epididymis, and seminal vesicle in rats with kidney yang deficiency, and the total glucosides of cistanche phenethyl alcohol have a more significant effect on the increase of wet weight of testis and seminal vesicle in rats.

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Regarding the improvement of related indicators of kidney-yang deficiency syndrome, this experiment has been conducted to determine the value of cAMP/cGMP, one of the pathological features of Yang-deficiency syndrome. . The results showed that all extracts of Cistanche can increase the value of cAMP/cGMP and regulate the neuroendocrine function of hypothalamus in rats with kidney-yang deficiency.

Further research on its possible upstream mechanism found that the function of each extraction part of Cistanche is closely related to the improvement of the HPG axis. 1O0. shut . The HPG axis plays an important role in the development and regulation of the animal reproductive system, almost controlling the entire process of mammalian development from fetal development, puberty to sexual maturity.” Hypothalamus secretes GnRH to regulate the release of pituitary FSH and LH, FSH and LH regulate sex hormones T, E At the same time, sex hormones carry out negative feedback regulation on the pituitary gland and hypothalamus. The feedback loop formed by the hypothalamus, pituitary gland, and gonads together maintain the stability of the endocrine system, and abnormal HPG system can lead to animal reproductive performance defects.'Kidney Yang Deficiency Syndrome One of the pathological bases is HPG axis dysfunction and hypofunction', which in turn affects the secretion of GnRH and insufficient T secretion, and ultimately leads to the decline of sexual function and spermatogenic dysfunction. Experimental results show that the total oligosaccharides of Cistanche can regulate FSH in rats with kidney-yang deficiency Total polysaccharides have a regulatory effect on E, FSH, and LH in rats with kidney-yang deficiency; and the total glycosides of cistanche phenethyl alcohol have a significant regulatory effect on the levels of sex hormones in rats with kidney-yang deficiency caused by hydrocortisone, and have significant effects. The amplitude increased the serum T level, which in turn improved the sexual performance of rats with kidney-yang deficiency.

According to literature reports, the total oligosaccharides of Cistanche are the medicinal substances of Cistanche for laxative and laxative, but there are few studies on its other pharmacological effects. The total polysaccharide of Cistanche has immune regulation, anti-aging, and other pharmacological effects. Its mechanism is related to scavenging free radicals and improving the antioxidant capacity of mitochondria. Cistanche phenethyl alcohol total glycosides show sex hormone-like effects and have multiple effects such as anti-oxidation, improvement of immune function, treatment of spermatogenesis disorders, improvement of learning and knowledge, and protection of cardiac muscle ischemia. Phenyl ethanol glycosides are a type of glycoside compound formed by the combination of phenyl ethanol aglycone and sugar base. Echinacoside and verbascoside in Cistanche are used as the index components of Cistanche to control the quality of medicinal materials. Reports have shown that the ergosteroside (i.e. verbascoside) in Cistanche tubulosa shows androgen-like effects, thereby improving sexual function. JIANG et al. found that echinacoside can protect rat sperm and sperm by regulating the synthesis of steroid hormones. The testicles are protected from damage. In addition, Phenylethanol glycoside extract can improve and restore the structure and morphology of seminiferous epithelium after reproductive injury, and it has an obvious protective effect on the structure and function of the sperm membrane. By comparison, it is found that the total oligosaccharides of Cistanche deserticola have obvious effects on the function regulation of the intestinal tract and the total polysaccharides on the regulation of the immune function of the body. As the main medicinal substance of the cistanche phenethyl alcohol glycosides, it has a prominent advantage in regulating the neuroendocrine system. It has a significant effect of invigorating the kidney and strengthening the yang to improve sexual ability.

In this experiment, the kidney yang deficiency model induced by hydrocortisone... was used to evaluate the effects of the different extraction sites of Cistanche cistanche total oligosaccharides, total polysaccharides, and total glycosides of phenyl ethyl alcohol on the improvement of the sexual ability of rats with kidney yang deficiency models. The results showed that each extraction part of Cistanche can improve the erectile latency and mating ability of kidney-yang deficiency rats, increase the wet weight of testis, epididymis, seminal vesicle, and the content of seminal berry sugar, increase the plasma cAMP/cGMP value, and regulate serum hormone levels. Improve the pathological morphology of the testis in rats with kidney-yang deficiency. As the main medicinal component of Cistanche deserticola extract, the total phenethyl alcohol glycosides have a more significant effect on the regulation of serum hormone levels. In particular, it can increase the serum T level of rats with kidney-yang deficiency, and significantly improve the sexual ability of rats with kidney-yang deficiency. This experiment provides experimental evidence for the elucidation of the mechanism of Cistanche for nourishing the kidney and strengthening yang and the development of subsequent drugs.

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