Experimental Study On The Nourishing Effect Of Cistanche Phenylethanoid Glycosides

How do Cistanche Phenylethanoid Glycosides improve memory?

Keywords: Cistanche; Phenylethanol glycosides; Learning and memory

Cistanche is called Goblin, Dayun, etc. It is also called "Chagangaoya" in Mongolian. It was first published in "Shen Nong's Materia Medica" and was listed as the top grade. There are about 18 species of meat ash in the world. There are about 4 species and 1 variant. Modern pharmacological research has found that meat ash paste has the functions of enhancing the body's immune function, promoting memory, and anti-aging. This article mainly studies the effect of Cistanche Phenylethanoid Glycosides on the learning and memory ability of mice, establishes a model of learning and memory impairment caused by scoreline, observes and evaluates its nootropic effects, and expounds the mechanism of action. To study the effects of Methoxin on Acetylcholinesterase and Antioxidant Enzymes.

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1 Material and methods

1.1 Medicinal materials, test agents, and main instruments

The meat puree was purchased from the pharmacy of the Inner Mongolia Zhongmeng Hospital, and the hydrobromide was purchased from the National Institute for the Control of Pharmaceutical and Biological Products. The other reagents were of national analytical grade. The rotary evaporator was purchased from Shanghai Yarong Biochemical Instrument Co., Ltd.; the square water maze was provided by the Institute of Pharmaceutical Research, Chinese Academy of Medical Sciences; the Morris water maze equipment and behavior record analysis software were provided by Shanghai Jiliang Software Technology Co., Ltd.;

1.2 Experimental animals

Clean-grade Kunming mice, weighing 20±2g, half male and half, were purchased from the Experimental Animal Center of Inner Mongolia University (animal number YXQ (Mongolia) 2002-0001), drinking and eating freely, alternating light and dark for 12 hours.

1.3 Preparation of Rouhuanrong phenethyl alcohol shake

Take 30g of dried meat ash paste, crush it and pass it through a 20-mesh sieve. Add 8 times the amount of 70% ethanol, extract with 70Y, extract twice, combine the two extracts and freeze-dry it at low temperature.

1.4 The grouping of experimental animals and the establishment of a model of learning and memory impairment caused by Scopoletin

Screen the mice in advance, and select the animals found on the platform in 120 sec for the experiment. The mice were randomly divided into 5 groups with 15 mice in each group, namely the normal group, the East Shizhu base model group, and the three-dose groups (10m" kg, 20mg/kg, 40mg/kg) of Cistanche Phenylethanoid Glycosides. The establishment of the model of learning and memory dysfunction caused by serotonin was carried out by reference method (4).

1.5 Channel (square) water maze behavior test

Each model and control group animals were subjected to a water maze experiment in turn. The path on the first d included 1 blind end, on the 2nd day there were 2 blind ends, on the 3rd day there were 3 blind ends, and 4, 5, 6, and 7 days were all four. Blind end. Train twice a day, and record the number of times each animal enters the blind end during swimming and the swimming time. If it does not go ashore within 120 s, it is calculated as 120 s.

1.6 Morris water maze behavior test

Reference method ⑸, measures the proportion of swimming time in the quadrant where the original platform is located in 120s, the number of times that it crosses the location of the platform (ie, the number of shuttles), and the first time to reach the original platform, and calculate the latency.

1.7 Determination of SOD activity and MDA in brain tissue and determination of cholinesterase content in the cerebral cortex

Refer to the kit instructions to take the brain tissue and detect the acetylcholinesterase activity. Another part of the animals collected whole blood and brain tissues according to the kit instructions to detect the content of MDA and SOD.

1.8 Statistical analysis

The data are expressed as Mean ± S, the difference between each group is compared with Excel software, and the comparison between groups is used and tested. P<0.05 is considered as a significant difference.

2. Experimental results

2.1. The effect of Rongrong phenylethanoid glycosides oxime on the learning and memory impairment model small K

2.11. Square water maze results

The results of the experiment are shown in Table 1. In the square water maze experiment, the number of errors and the time required to find the endpoint of the mice in the scoring model group are significantly different from those in the normal group (P v 0.01). Glycoside has a significant effect on improving learning and memory. In the 20mg/kg and 60ra&/kg groups, it can significantly reduce the number of collisions (P<0.05) and significantly shorten the time to find a safe island (P<0.05).

2.2 The effect of Cistanche phenylethanoid glycosides on MDA, SOD, and acetylcholine as activities in the brain group of model mice

Cistanche phenylethanoid glycosides can improve the SOD activity in brain tissues and significantly reduce the content of MDA, suggesting that Cistanche phenylethanoid glycosides have anti-lipid peroxidation effects. Scopolamine can significantly increase the AchE content in mouse brain endothelial tissues, while the scopolamine group can significantly reduce the activity of acetylcholinesterase.

3. Discussion

Learning and memory are the high-level functions of the brain and the requirement for intelligence. Scopolamine is a central anticholinergic drug. By blocking the central M choline receptors, it inhibits the short-term memory of mice after learning and learning. It acts on the link of memory acquisition and causes similar memory and cognitive impairment models. Cistanche phenylethanoid glycosides are effective for scopolamine. The resulting learning and memory dysfunction has a very good therapeutic effect. In the square water maze experiment, the administration of Cistanche phenylethanoid glycosides can significantly shorten the time to find the platform and reduce the number of errors; in the Morris water maze experiment, The Cistanche group can significantly shorten the incubation period and the time to find a platform.

MDA reflects the degree of lipid peroxidation damage in the body. After the administration of scopolamine, it significantly increases the content of MDA in the brain and significantly reduces the activity of SOD, suggesting that the learning and memory impairment caused by scopolamine is related to lipid peroxidation in the brain. Significant anti-lipid peroxidation effect. At the same time, this is also one of the pharmacological bases for the treatment of Alzheimer's disease. Acetylcholine plays an important role in learning and memory. Ach is catalyzed and synthesized by ChAT in the brain and decomposed by AChE to form the metabolic cycle of ACh. In the scopolamine model group, AChE was abnormally increased in the cerebral cortex, which caused the protruding gap ACH to be hydrolyzed, which caused the animal's ability to learn and remember to decrease rapidly. However, the activity of ACHE could be significantly reduced after the administration of glutinous rice phenethyl alcohol ridge. Make the ACH of the protruding gap recover.

In short, this experiment found that Cistanche phenylethanoid glycosides can significantly improve learning and memory functions, and this improvement of learning and memory is related to anti-brain lipid peroxidation and reducing ACHE activity. The research on the improvement of learning and memory functions of Cistanche. The further development of medicinal plants improves the pharmacological basis.