1. Why this matters: Aging, fatigue, and men's vitality as one connected challenge

Modern biology suggests that aging is closely associated with multiple molecular mechanisms. Among them, mitochondrial functional decline and disordered cellular energy metabolism are often regarded as key drivers. As the cell's "power plant," mitochondria are responsible for producing adenosine triphosphate (ATP)-the body's "energy currency." When mitochondrial activity decreases, ATP production drops, cellular repair weakens, oxidative stress rises, and signs of aging can appear, such as reduced skin elasticity, memory decline, and diminished physical performance.

At the same time, fast-paced modern life and increasing stress contribute to a growing proportion of people in a sub-healthy state. Fatigue and declining male function are common manifestations-signals that can precede disease. Existing drug-based approaches may show rapid and noticeable effects, but concerns about side effects, dependence, and long-term suitability limit their broader use.

Many anti-aging or anti-fatigue products on the market rely on single active ingredients (e.g., only resveratrol), which can be insufficient for multi-target, multi-pathway support. As a result, there is a clear need to develop solutions that can support anti-aging, anti-fatigue, and male vitality in a more integrated way-while also being adaptable into health products (such as tonic wines).

 

 

Cistanche Extract Benefits

 

2. Overview of the invention: A TCM composition + tonic wine + preparation method + applications

This article introduces:

A traditional Chinese medicine composition (multi-herb formula),

A tonic wine (health wine) containing this composition,

A preparation method (including extraction, filtration, optional freeze clarification and fine filtration),

Applications for anti-aging, anti-fatigue, and improving male function, as well as antioxidant support and relief of physical fatigue.

A key feature is that the ingredients are designed to create a notable synergistic effect-meaning the combined performance is stronger than what each herb would achieve independently.

 

3. The herbal composition (by weight parts)

3.1 Core formula ranges (broad range)

The TCM composition is made from the following raw materials, in parts by weight:

Lycium barbarum fruit (Goji berry): 50–99 parts

Dioscorea (Chinese yam): 20–40 parts

Rubus chingii fruit (Fu Pen Zi / Raspberry-type Rubus): 15–27 parts

Asparagus cochinchinensis root (Tian Dong): 10–20 parts

Ophiopogon japonicus (Mai Dong): 10–20 parts

Longan aril (Long Yan Rou): 10–20 parts

Alpinia oxyphylla fruit (Yi Zhi Ren): 3–9 parts

Prepared Rehmannia root (Shu Di Huang): 3–9 parts

Codonopsis (Dang Shen): 1–8 parts

Ziziphus jujuba seed (Suan Zao Ren): 1–8 parts

Angelica sinensis (Dang Gui): 1–8 parts

Cistanche (Rou Cong Rong): 1–8 parts

Ginseng (Ren Shen): 1–3 parts

3.2 Preferred formula ranges (optimized range)

Preferably, the formula uses:

Goji berry: 63–80 parts

Chinese yam: 25–35 parts

Rubus chingii: 17–23 parts

Asparagus root: 11–17 parts

Ophiopogon: 11–17 parts

Longan aril: 11–17 parts

Alpinia fruit: 3–8 parts

Prepared Rehmannia: 3–8 parts

Codonopsis: 2–5 parts

Ziziphus seed: 2–5 parts

Angelica: 2–5 parts

Cistanche (Rou Cong Rong): 2–6 parts

Ginseng: 1 part

3.3 Optional pharmaceutical carriers/excipients and dosage forms

The composition may also include pharmaceutically acceptable carriers or excipients, such as (one or more of): solvents, diluents, disintegrants, precipitation inhibitors, surfactants, glidants, binders, lubricants, dispersants, suspending agents, isotonic agents, thickeners, emulsifiers, preservatives, stabilizers, hydrates, emulsification accelerators, buffers, absorbents, colorants, flavoring agents, sweeteners, ion-exchange agents, mold-release agents, coating agents, taste-masking agents, and antioxidants.

The composition can be prepared as pharmaceutically acceptable forms including: granules, decoctions, powders, tablets, capsules, pills, emulsions, suspensions, wines, or tinctures.

 

4. The tonic wine: what it is

The tonic wine includes:

A base alcohol (liquor), and

The above TCM composition.

 

Cistanche Wine

Click to check the listing of cistanche wine

Supportive Service of Wecistanche- For more details about cooperation
Email:wallence.suen@wecistanche.com

 

 

5. Preparation method (for product developers and OEM/ODM customization)

5.1 Basic method (essential steps)

Weigh the raw herbal components, mix uniformly, and add them to a base liquor for extraction (maceration).

After extraction, filter to obtain the liquor extract.

Recommended filtration precision for the first filtration: 50 μm to 180 μm.

5.2 Optional advanced clarification & fine filtration (for improved stability and appearance)

After obtaining the liquor extract:

Freeze clarification at −7∘C-7^\circ C−7∘C to −15∘C-15^\circ C−15∘C for 22–26 hours;

Conduct fine filtration, then let stand at room temperature for 2–4 days;

Adjust alcohol content (ABV/proof), then fine filter again to obtain the final tonic wine.

Fine filtration precision: 0.2 μm to 0.5 μm
Final alcohol degree: 36° to 52° (Chinese liquor degree system).

5.3 Base liquor requirements and extraction ratio

Base liquor alcohol degree: 50°–60°

Total mass of raw materials : volume of base liquor = (109–313) g : 1 L

Preferably: (150–250) g : 1 L

More preferably: (180–200) g : 1 L

Extraction is performed as sealed maceration for 20–23 days, during which the mixture is circulated uniformly every day.

A practical circulation approach is described as: open the bottom outlet valve and top return valve, start a circulation pump, draw liquor from the bottom and spray/return from the top, forming a closed-loop circulation to drive whole-tank movement. Alternatively, shaking can be used. An example operation is twice daily, 10–30 minutes each time.

5.4 Base liquor composition and room temperature definition

Base liquor is fermented baijiu containing:

Ethanol 50–60 wt%

Water 38–48 wt%

Trace flavor substances (esters, acids, etc.) 1–2 wt%

"Room temperature" refers to 20–30°C.

5.5 Alcohol adjustment example

If the base liquor is 52°, take 1 part of the fine-filtered extract and add about 0.24 parts purified water, circulate/mix uniformly and allow to stand to obtain a target alcohol degree of 42° ± 1°.

 

6. Applications (claims-style, but explained for real-world product planning)

6.1 Pharmaceutical application

The composition can be used in preparing drugs for:

Anti-aging

Anti-fatigue

Improving male function

6.2 Health product / functional food application

The composition can be used in preparing health products that help:

Antioxidant support

Relief of physical fatigue

The health product may include tonic wine, and may also exist as functional foods, foods, or food additives.

 

7. Why Cistanche (Rou Cong Rong) belongs in a formula like this (brand-friendly explanation)

In traditional formulation logic described in the text, the herbs are positioned to support complementary actions:

Several herbs act as the "core" to support kidney essence and strength (conceptually aligned with vitality).

Others nourish qi and yin, support fluids, and calm the mind.

Cistanche (Rou Cong Rong) is positioned to warm and support kidney function and secure essence, contributing to the overall vitality direction of the formula and balancing the approach so it is not purely one-sided.

In product development terms: Cistanche is often selected when brands want a formula positioned toward energy, stamina, and men's vitality, while still remaining within a broader "anti-fatigue / healthy aging" narrative.

 

 

 

 

8. Experimental framework (zebrafish model): anti-aging, anti-fatigue, and male function

The document reports zebrafish-based functional evaluations to demonstrate:

Anti-aging (telomerase activity) using a hydrogen peroxide-induced aging model

Anti-fatigue (total movement distance) using a sodium sulfite-induced fatigue model

Male function improvement (sperm count and testosterone) using a cyclophosphamide-induced oligospermia model

Note: The excerpt references multiple tables and figures (e.g., Table 1–7, Figure 1–3), and mentions results comparisons between examples and comparative examples. However, the specific numeric table data are not fully shown in the text you provided here. Therefore, this blog version summarizes the test design and reported conclusions without fabricating missing numeric values.

8.1 Anti-aging test (telomerase activity)

Test animals: wild-type AB strain zebrafish embryos (6 hpf)

Aging model: hydrogen peroxide exposure

Endpoint: telomerase activity measured by ELISA kit

Conclusion described: the tonic wines prepared in the examples show anti-aging efficacy; when some ingredients fall outside the described ranges (comparative examples), telomerase activity decreases, indicating weakened anti-aging action.

8.2 Anti-fatigue test (total movement distance)

Test animals: 4 dpf zebrafish

Fatigue model: sodium sulfite exposure

Endpoint: total movement distance measured by behavioral analysis system

Conclusion described: example tonic wines increase total movement distance; deviations from the formula ranges reduce performance.

8.3 Male function improvement test (sperm count & testosterone)

Test animals: 6 mpf male zebrafish

Model: cyclophosphamide-induced oligospermia

Endpoints: sperm staining + image analysis; testosterone ELISA

Conclusion described: example tonic wines improve sperm count and testosterone metrics relative to the model; deviations from formula ranges reduce improvement.

9. Example manufacturing process (clear, factory-friendly SOP style)

Example 1 (tonic wine, 1 L batch)

Herbs (grams):

Goji berry 75 g

Chinese yam 30 g

Rubus chingii 20 g

Asparagus root 15 g

Ophiopogon 15 g

Longan aril 15 g

Alpinia fruit 5 g

Prepared Rehmannia 5 g

Codonopsis 3 g

Ziziphus seed 3 g

Angelica 3 g

Cistanche 3 g

Ginseng (artificially cultivated, under 5 years) 1 g

Base liquor: 1 L, 52°

Total herb mass : liquor volume = 193 g : 1 L

Sealed extraction for 21 days

During extraction: shake to circulate twice daily (AM/PM), 10–30 minutes each time

Filtration & clarification:

Coarse filtration at room temperature, 100 μm

Freeze at −10∘C-10^\circ C−10∘C for 24 h

Fine filtration at 0.35 μm

Stand at room temperature for 3 days

Adjust alcohol degree, mix, and fine filter again at 0.35 μm

Obtain tonic wine at 48°

10. Alternative dosage forms (for brands beyond tonic wine)

Example 6 (tablet form)

Add 8× purified water, reflux extract twice (1.5 h each)

Filter and combine filtrates

Vacuum concentrate (60–70°C, −0.08-0.08−0.08 MPa) to a thick paste with relative density 1.15–1.25

Vacuum dry, grind, pass 80-mesh

Blend with microcrystalline cellulose, lactose, silica (ratio: dry extract : MCC : lactose : silica = 1 : 0.4 : 0.2 : 0.05)

Use 75% ethanol as binder, granulate (16 mesh), dry (60°C), size (14 mesh)

Add magnesium stearate (0.5% of granule weight), compress into 0.5 g tablets, film coat, pack

Example 7 (granules)

Add 8× purified water, reflux extract twice (1.5 h each)

Filter; vacuum concentrate (60°C, −0.08-0.08−0.08 MPa) to a clear paste of relative density 1.08–1.12

Add dextrin and lactose (ratio: clear paste : dextrin : lactose = 1 : 0.3 : 0.2)

Add purified water to form soft material, wet granulate (16 mesh)

Hot-air dry (65°C), size (10 mesh), pack

11. Ingredient compliance note (as stated in the document)

The document states that, except for ginseng, all herbs are included in the officially published Chinese list of substances that are both food and traditional Chinese medicinal materials (including new additions in 2024), described as having no toxic side effects. It also states ginseng (artificially cultivated) is approved as a new food resource within specified dosage limits.

For international markets (EU/US/SEA), regulatory status can differ by jurisdiction and claim type. If you plan to sell or market with specific health claims, consult local regulatory counsel and align labeling accordingly.

 

12. Customization for your brand (Cistanche factory direct)

If you are building a Cistanche-centered product line, this formula and process can be customized along multiple dimensions:

Product form: tonic wine, tincture, granules, tablets, capsules

Alcohol degree: 36°–52° target range

Extraction ratio: (109–313) g : 1 L (with preferred windows)

Filtration & stability: optional freeze clarification + 0.2–0.5 μm fine filtration for clarity and long-term stability

Positioning: healthy aging support, energy & stamina support, antioxidant support, physical fatigue relief, men's vitality support

If you'd like, tell me your target market (China domestic / US / EU / Middle East), desired dosage form, and whether you want "stronger men's vitality angle" or "balanced anti-fatigue + healthy aging angle"-I can tailor the copy and structure to match your compliance and branding needs.