Development Of Enriched Oil With Polyphenols Extracted From Olive Mill Wastewater Part 1

Abstract: The extraction of olive oil produces significant residual olive mill wastewater (OMW). The composition of OMW varies according to the type of olive, the fruit’s ripeness level, and the extraction method (traditional one-pressing system or continuous systems based on centrifugation of the olive pulp). In olive-producing countries, OMW is important for the environment and the economy and is also a low-cost source of polyphenolic compounds with high antioxidant properties. Olive oil’s properties, such as its anti-atherogenic, anti-inflammatory, anti-aging, and immunological modulator effects, have been attributed to its polyphenols. In this study, the cloud point extraction (CPE) method was used to recover polyphenolic compounds from OMW. The total micellar phase of the three recoveries was 24.2% and the total polyphenols (after sonication) was 504 mg GAE/Kg. Furthermore, the addition of polyphenols recovered from OMW enhanced the olive oil and extended its shelf life without changing its organoleptic properties. There was a 42.2% change in polyphenols after 0.5% enrichment of micellar dispersions. Thus, it is suggested that the CPE method could lead to better waste management in the olive oil industry and improve the nutritional quality of food products.

Glycoside of cistanche can also increase the activity of SOD in heart and liver tissues, and significantly reduce the content of lipofuscin and MDA in each tissue, effectively scavenging various reactive oxygen radicals (OH-, H₂O₂, etc.) and protecting against DNA damage caused by OH-radicals. Cistanche phenylethanoid glycosides have a strong scavenging ability of free radicals, a higher reducing ability than vitamin C, improve the activity of SOD in sperm suspension, reduce the content of MDA, and have a certain protective effect on sperm membrane function. Cistanche polysaccharides can enhance the activity of SOD and GSH-Px in erythrocytes and lung tissues of experimentally senescent mice caused by D-galactose, as well as reduce the content of MDA and collagen in lung and plasma, and increase the content of elastin, have a good scavenging effect on DPPH, prolong the time of hypoxia in senescent mice, improve the activity of SOD in serum, and delay the physiological degeneration of lung in experimentally senescent mice With cellular morphological degeneration, experiments have shown that Cistanche has the good antioxidant ability and has the potential to be a drug to prevent and treat skin aging diseases. At the same time, echinacoside in Cistanche has a significant ability to scavenge DPPH free radicals and can scavenge reactive oxygen species, prevent free radical-induced collagen degradation, and also has a good repair effect on thymine free radical anion damage.

Click on Anti-aging Cistanche Para Que Serve

【For more info: david.deng@wecistanche.com / WhatApp:86 13632399501】

Keywords: antioxidants; cloud point extraction; food industry; enrichment; olive mill wastewater;  olive oil; polyphenols; recovery

1. Introduction

The demand for olive oil is continuously increasing worldwide. In the Mediterranean area, which is the biggest olive-producing area worldwide, a huge amount of agro-industrial wastewater is generated from the olive processing industry [1,2]. Olive mill wastewater (OMW) residues are composed of solid waste—consisting of olive pulp—as well as liquid waste—consisting of vegetables and additional water generated during decantation. OMW has a dark-brown color (that can become black) and a distinctive,  potent aroma reminiscent of olives [3]. The olive mill by-products have environmental and economic significance in olive-producing countries. OMW creates high levels of pollutants because of its high organic load and a high content of phytotoxic and antibacterial polyphenolic substances, which resist biological degradation [4]. The treatment and disposal of OMW are becoming a serious environmental problem. Thus, olive oil-producing countries have been facing a serious challenge to find an environmentally friendly and economically viable solution to the handling and disposal of OMW.

An innovative and environmentally friendly technology for extracting bioactive substances, particularly from food, is cloud point extraction (CPE). It is a technique where chemical or biological components are extracted using non-ionic surfactants that when heated to (or above) a critical temperature, tend to separate from the bulk solution and create clouds [5]. Surfactants are specifically employed as extractants during the CPE  process. In the right circumstances, the extraction takes place at a temperature below the cloud point—when the surfactant becomes cloudy (i.e., less soluble than the initial sample or even insoluble)—leading to the separation of two phases: the aqueous phase and the surfactant-rich phase. Due to clouding processes, the technique leads to a preconcentration of analytes (such as polyphenols) in the surfactant-rich phase [6]. Furthermore, it has been demonstrated that the compounds solubilized by the micelle were protected from oxidation [7].

Polyphenolic compounds, sugars, and organic acids make up the majority of OMW. OMW also has rich resources such as potassium and other mineral nutrients, which may be utilized again as fertilizer [8]. However, OMW could be a natural source of antioxidants due to its high content of polyphenolic compounds. Olive oil polyphenols have potentially beneficial nutritional properties for their antioxidant and biological activities such as anti-allergic, anti-inflammatory, anti-cancer, and anti-hypertension [9,10]. The low redox potential of polyphenols, which enables them to act as reducing agents by donating hydrogen or electrons and scavenging free radicals, is the basis of their antioxidant activity [7].

Oil producers aim to produce food products that keep their nutritional value and shelf life over an extended length of time. These factors render antioxidant supplementation a common technique for lowering lipid oxidation in food production [11]. To prevent oxidation in foods containing fats and oils, synthetic antioxidants including butylated hydroxytoluene (BHT), tert-butyl hydroquinone (TBHQ), and butylated hydroxyanisole (BHA) have been employed as food additives. However, it was revealed that these chemical antioxidants have been linked to a variety of health hazards, including cancer and carcinogenesis [12].

Recent research has shown that natural antioxidants can prevent edible oil oxidation [11–13]. The enrichment of olive oil with polyphenolic substances can increase the shelf life of olive oil without affecting its organoleptic characteristics. However, the antioxidant/pro-oxidant balance depends on many factors, particularly dose (e.g., concentration) and timing (e.g., storage duration) and it is a very delicate process that can readily be changed [14]. Consequently, the recovery of polyphenolic compounds not only provides an economic opportunity but also lowers the environmental charge of wastewater.

The aims of this study were first to recover the polyphenolic substances from OMW  with the application of the CPE method, and secondly to enrich olive oil with the recovered polyphenolic substances. The stability of the polyphenols is an important parameter in this enrichment. Emulsifiers, such as lecithin, were studied for this purpose.

2. Materials and Methods

2.1. Materials

In this investigation, a sample of olive mill wastewater (Olea europaea var. Koroneiki)  from the Preveza region (Epirus, Greece) was employed. The OMW sample was specifically collected by the olive oil industry, frozen, and delivered to our lab the same day. Olive oil from a local market in Karditsa (Thessaly, Greece) was used as the matrix for the control sample, composed of virgin and refined olive oils.

2.2. Reagents

The 2,2-Diphenyl-1-picrylhydrazyl (DPPH• ) and α-tocopherol were purchased from Alfa Aesar (Karlsruhe, Germany). Sodium chloride, citric acid, lecithin of soya (>97%),  diethyl ether, and isooctane were obtained from Carlo Erba (Milano, Italy). Sodium carbonate anhydrous and sodium hydroxide were from Penta (Prague, Czech Republic). Absolute ethanol, methanol, Folin–Ciocalteu reagent, and gallic acid monohydrate were from Panreac (Barcelona, Spain). Cyclohexane and n-hexane were from Sigma-Aldrich (Steinheim, Germany). The deionized water used in the experiments was produced using a deionizing column. 

2.3. Extraction of Polyphenols from OMW 

A method called cloud point extraction (CPE) was used. The CPE method is based on the notion that when a surfactant’s concentration in a solution exceeds that of the critical micellar dispersion, it can generate micellar dispersions [6]. Figure 1 shows the experimental steps of the CPE method. First, the sample had to be defrosted before the solids could be separated using a centrifuge (Digicen 20-R, Orto Alresa, Madrid, Spain) for 10 min at 4500 rpm. After collecting the supernatant, citric acid (50%) was added to bring the pH level to 3.5. The entire sample was divided into three portions, with lecithin as a surfactant added to each of them in concentrations of 1%, 3%, and 5%, respectively, along with 30% salt. An intense magnetic stirrer was used for agitation. After 20 min, the solution was centrifuged for 5 min at 4500 rpm. The upper micellar (surfactant) phase (SP) and the lower aqueous (water) phase (WP) were examined separately. The first recovery of the polyphenolic dispersions occurred at this point. With the water phase containing the non-extracted analytes, the CPE method was performed two more times under the same conditions (2nd and 3rd recovery). Foods 2023, 12, x FOR PEER REVIEW 3 of 13 Absolute ethanol, methanol, Folin–Ciocalteu reagent, and gallic acid monohydrate were from Panreac (Barcelona, Spain). Cyclohexane and n‐hexane were from Sigma‐Aldrich (Steinheim, Germany). The deionized water used in the experiments was produced using a deionizing column

2.4. CPE Method Performance Determination 

2.4.1. Total Polyphenol Content

According to Chatzilazarou et al. [15], the Folin–Ciocalteu method was used to determine the total polyphenol content (TPC) photometrically. Briefly, 0.5 g of micellar dispersions and 0.5 mL of Folin–Ciocalteu reagent were added to a 25-mL volumetric flask. After 3 min, 1 mL of Na2CO3 (35%, w/w) was added. Deionized water was used to fill the flasks to the necessary volume level, and they were then kept in the dark for 60 min. A Shimadzu UV-1700 UV/Vis spectrophotometer (Kyoto, Japan) was used to measure absorption at 750 nm. Standard solutions (1–10 mg/L) were used to create a standard gallic acid curve. TPC was expressed as mg gallic acid equivalents (GAE) per Kg of micellar dispersions.

The DPPH method of Katsoyannos et al. [16], with some modifications, was used to evaluate the radical scavenging activity of the polyphenols extracted in the surfactant phase as well as those still present in the sample following the CPE treatment (water phase). In short, a 1 mL, 0.1 mM solution of methanolic DPPH was added to 4 mL of the sample solution (500, 1000, and 2000 mg/L). The mixture was thoroughly shaken before being let to stand at room temperature in the dark for 30 min while the absorbance was measured at 517 nm. The formula used to determine the % inhibition was: % Inhibition = 100 × (Acontrol − Asample)/Acontrol, where Acontrol and Asample are the absorbances. From the scavenging activities (%) vs concentrations of the corresponding sample curve, the IC50  was determined.

2.5. Enrichment of Olive Oil with Micellar Dispersions 

Using an ultrasonic probe (Misonix Sonicator S3000, Qsonica, LLC, Newtown, CT, USA) and a sonication protocol (75% power energy for 60, 75, and 90 min with 0.5 pulse cycles), micellar size reduction was investigated. The CPE method was used to recover micellar dispersions, which were then added to the olive oil to improve it. For enrichment,  concentrations of 0.5, 1, and 5% were employed.

2.6. Enriched Olive Oil Quality Analysis

According to Commission Regulation (EEC) No. 2568/91 [17], the method for determining free fatty acids (FFAs) in olive oil was developed. 

2.6.2. Refractive Index 

A refractometer (Quartz/Digital Abbe refractometer, Medline Scientific Limited, Oxon, UK) was used to measure the sample’s refractive index. The instrument was calibrated with distilled water, which had a refractive index of 1.3333 at 20 ◦C.

2.6.3. Colorimetry 

The colorimeter was used to determine the color of the samples of olive oil (Lovibond CAM-System 500, The Tintometer Ltd., Amesbury, UK). To determine CIELAB  color, a sample (25 mL) was placed in a 50 mL beaker and placed in the colorimeter. A  psychometric index of lightness, L*, and chrominance coordinates, a* and b*, were defined. Additionally, the formula for calculating chroma, or C*, was as follows: C* = [(a*)2 + (b*)2 ] 1/2 .

2.6.4. Spectrophotometric Investigation in the Ultraviolet 

Utilizing a spectrophotometer, the method of ultraviolet spectrophotometric investigation was carried out following the Commission Implementing Regulation No 299/2013 [18].

2.6.5. Rancimat Method 

According to Lalas et al. [19], the Rancimat method was used to test the oxidation stability of the olive oil samples. More specifically, the reaction vessels of the Rancimat 743 (Metrhom LTD, Herisau, Switzerland) were filled with around 3 g of each olive oil. The chosen parameters were a 90 ◦C temperature and a 15 L/h airflow. The point when the rate of oxidation changes quickly and produces a strong inflection point on the oxidation curve is known as the induction time, and it is represented by the value of the olive oil stability index. This was the induction period (IP), which was provided in hours. Additionally, this formula was used to compute the protection factor (PF): PF = (IP with antioxidant)/(IP  without antioxidant). Inhibition of lipid oxidation is indicated by a protection factor larger than one, and the antioxidant activity is improved by a higher PF value.

2.6.6. Differential Scanning Calorimetry

Differential Scanning Calorimetry (DSC) measures the temperatures and heat flows associated with transitions in materials as a function of time and temperature. These measures include both quantitative and qualitative data about physical and chemical changes involving endothermic or exothermic processes, as well as alterations in heat capacity. DSC was used in this investigation to assess the oil’s oxidative stability [20]. A  special aluminum pan was used to weigh 5 mg ± 0.5 mg of oil. A blank pan (reference) was added as well. Oxygen flow was 20 mL/min, and oxidative conditions were programmed at Diamond DSC (PerkinElmer Inc., Shelton, CT, USA). The sample and reference were placed on the DSC, and the thermogram was initiated at 40 ◦C for 1 min, followed by 300 ◦C  at a rate of 10 ◦C/min. The onset temperature of the oxidation peak (Tmax) determines the starting temperature of oxidation.

2.6.7. Extraction of Polyphenolic Compounds from the Enriched Olive Oils 

The extraction of polyphenols from the olive oils was performed as described by Kalantzakis et al. [21]. Olive oil samples (1 g) were dissolved in 2 mL of n-hexane and 2 mL of a 60:40 (v/v) methanol/water solution. The final solution was centrifuged at 4500 rpm for 5 min after vigorous agitation. The soluble in methanol/water (polar) fraction of olive oil samples was obtained and used as it was.

2.6.8. Total Polyphenol Content 

As previously mentioned, the total polyphenol content (TPC) was determined using the Folin–Ciocalteu method. TPC was expressed as mg gallic acid equivalents (GAE) per Kg of olive oil.

2.6.9. Radical-Scavenging Activity 

The DPPH method, as previously mentioned, was used to evaluate the radical scavenging activity of the polyphenols extracted from the olive oil samples. The capacity to scavenge the DPPH radical was expressed as % inhibition, and the IC50 was calculated from the scavenging activities (%) vs. concentrations of the corresponding sample curve.

2.7. Statistical Analysis 

The results are given for the mean and standard deviation (SD) of three contemporaneous assays. Using IBM SPSS Statistics (Version 26) statistical software package (SPSS Inc., Chicago, IL, USA), a one-way ANOVA was performed to determine whether there were any statistically significant differences between the mean values. A significant level of p < 0.05 was utilized.

3. Results and Discussion

3.1. Recovery of Polyphenols 

A lecithin concentration aqueous phase was performed, where the same procedure was employed to recover polyphenols. Due to the low lecithin concentration of 1%, no surfactant micelle was produced. On the other hand, when there was a high concentration of 5% lecithin, this reduced their solubility in water and resulted in the formation of two phases. Both the equilibration temperature (45 ◦C) and the salt content (30%) were constant (third recovery). The total micellar phase of the three recoveries is expressed using the Total SP column (Figure 2).

Additionally, sonication procedures (75% power energy for 60, 75, and 90 min with a  pulse cycle of 0.5) with an ultrasonic probe were used to examine micelle size reduction. Finally, 0.5% of the polyphenol dispersions recovered by the CPE method were added to the oil to enhance it. This result can be explained by Víctor-Ortega et al. [22], who found that the solubilization of phenolic compounds on surfactant micelles became saturated at higher surfactant concentrations, and the polyphenols retention decreased. In addition,  according to Sliwa and Sliwa [23], when the surfactant concentration is below the critical micellar concentration (CMC), the solubility of the active substance is low.

In the Katsoyannos et al. [24] study, when 6% of Triton X‐114 surfactant was applied,  the CPE technique in OMW (after the removal of fatty compounds) produced recoveries higher than 60%. In a different study, Gortzi et al. [25], showed that utilizing simple and subsequent CPE, the total phenol recovery from OMW with various Genapol X‐080 concentrations (2, 5, and 20%, v/v) reached up to 89.5%. Additionally, Katsoyannos et al. [16],  used a double‐step CPE with 5% + 5% of Tween 80 to recover up to 94.4% of the total phenols from the OMW. Finally, in the Alibade et al. [6] study, lecithin was used as a  surfactant while CPE was used to recover phenolic compounds from wine sludge waste (WSL). The surfactant‐rich phases showed strong antioxidant activity. Their findings show that CPE and lecithin can be utilized to successfully separate polyphenols from WSL.

3.2. Enrichment of Olive Oil with Polyphenols from Olive Oil Wastewater 

An alteration in the organoleptic properties of the micellar dispersions was observed after 0.5% w/v enrichment (turbidity). This modification led to the final selection of 3%  lecithin enrichment. To obtain better outcomes, the first recovery was combined with the second and third ones. Additionally, sonication proved successful in reducing micellar dispersions. The oil system responded negatively to amounts of 1% and 5%. After a few hours of enrichment, the precipitate was seen in the oil samples.

The initial sample was subjected to the CPE method, which caused the polyphenol concentration to drop from 3448 mg to 346 mg GAE/Kg (Figure 3). At the molecular level,  the reduction in the number of hydrogen bonds between polyphenols and water allowed the incorporation of polyphenols into the micelle at the expense of its solubilization [26]. The micelles were then treated with sonication and methanol to extract the polyphenols,  increasing the polyphenol content to 1562 mg GAE/Kg. The final concentration of polyphenols was 504 mg GAE/Kg (after sonication), which demonstrates that a decrease in micellar dispersion results in a drop in the concentration of total polyphenols [27]. Additionally,  prolonged ultrasonic time causes particles to dissolve in the solvent, reducing the TPC  value [28].

【For more info: david.deng@wecistanche.com / WhatApp:86 13632399501】