Study On Anti-fatigue Effects Of Cistanche Deserticola Extract And Epimedium Extract

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Abstract： Objective To study the anti-fatigue effects of extract from cistanche deserticola and epimedium on mice. Methods Rotarod performance test and swimming test were applied in comparing anti-fatigue effects of extract from cistanche deserticola and epimedium and extractive from ginseng water on mice. Result in The dwell time during the rotarod performance test and swimming test of the test group were longer than those of control group# < 0.05). Conclusion The extract from cistanche deserticola and epimedium is efficient on the action of anti-fatigue.

Click to Cistanche herba for fatigue

[Key words ] Cistanche deserticola; Epimedium; Extractive; Anti-fatigue; Rotarod performance test; Swimming test

Cistanche is the dry fleshy stem with scale leaves of Cistanche deserticola YCMa or Cistanche tubulosa (Schrenk) Wight. It is mainly produced in Inner Mongolia, Xinjiang, Gansu, and Ningxia. In other places, the active ingredients of cistanche are phenethyl alcohol glycosides, betaine, and eggplant puree polysaccharides. It has the effects of nourishing kidney yang, nourishing essence and blood, and moisturizing the intestines. It is often used to treat impotence, infertility, waist, and knees. Soreness, weakness of muscles and bones, dry intestines, and constipation. Cistanche plays an active role in the treatment and health care of many aspects of the human body. It is called "desert ginseng". It is currently a hot spot in the research of functional foods and medicines. Yang Lei is a nourishing Chinese medicine, belonging to Epimedium L (Epimedium L) perennial medicinal plant (3). Its active ingredient Epimedium L total flavonoids mainly contain Lea and Epimedium polysaccharides (EPS). ), is a traditional Chinese medicine for invigorating kidney yang, strengthening muscles and bones, and dispelling rheumatism. It has good curative effects on male reproductive system diseases, high blood pressure, cardiovascular and cerebrovascular diseases, body immunity, and tumors. Both meat eggplant paste and Yinyanglei have anti-fatigue effects. They are the main anti-fatigue compound medicines such as the compound black ant Jianshen Jiujian, Fufang Yishen Granules, F3 anti-fatigue oral liquids, Yinyanglei, and cistanche. Both are among the top 13 single-medicine drugs used frequently. However, there has not been a report on the anti-fatigue study of the combined use of two Chinese medicines: Epiphyllum and Epimedium. In this experiment, the compound extracts of Epimedium and Roucong are used to study and explore their anti-fatigue effects through the experiment of running time and swimming time. It can provide an experimental basis for the development, application, and in-depth research of the compound and health care products.

1 Material and methods

1.1 Experimental animals

Kunming female mice 36R, body weight (20 ± 5) g, provided by Guangdong Medical Experimental Animal Center.

1.2 Medicines and reagents

Ginseng, Cistanche, Chinese wolfberry, Yinyanglei, Pueraria lobata root powder, etc. were all purchased from Guangdong Medical Materials Company, ethanol (Tianjin Yongda Chemical Reagent Development Center, Analytical Pure, 20090724) o 1.2.1 Ginseng Water Decoction ginseng 10g, cut into thin slices, soak in an appropriate amount of steamed water for 0.5 h, fry twice at medium heat, the first decoction is 30 min, the second decoction is 20 min, the two decoctions are filtered and mixed, and concentrated to 50 ml. For use, the concentration is 0.2g/ml.

1.2.1 The extract of Epiphyllum lucidum (Medicine I).

The meat is pulverized from the puree, and then extracted with 80T ultrasonic water for 2h, and then the extract is concentrated to 1 g (crude medicine)/ml; pulverized with 80-long After ultrasonic extraction for 2 hours, the solution was recovered and concentrated to 1 g (crude medicine)/ml. Mix the two concentrates and set them aside.

1.2.2 The method

Extracting meat from Epimedium medlar and Pueraria lobata powder complex (drug U) is the same as that of 1.2.2; the medlar was extracted with 80-long ultrasonic water for 2 hours, and then the extract was concentrated to 1 g (crude medicine)/ml; Pueraria lobata root powder is a fine powder. Take 10ml each of meat girl paste, wolfberry fruit, and Yin Yang Bud extract, add 10g kudzu root powder, mix well, add steamed stuffing water to adjust the solution to 40 ml, and set aside.

1.2.3 Equipment

Mouse cage, stopwatch, thermometer, mouse gastric applicator, electronic platform scale, mouse rotary rod fatigue meter Yidali UGO company, model 47600), mouse swimming pool (30cm x 16 cm x 18 cm), ultrasonic vibration (Shandong Jining Aobo Ultrasonic Electric Co., Ltd., JAC-300).

2. Experimental method

2.1 Mouse runner fatigue test 36 mice were randomly divided into blank control group, ginseng decoction group, and drugs according to body weight

Both group I and group II were administered ig, once a day, 0.4 ml each time, for one week. Half an hour after the last administration, start the mouse rotating rod fatigue meter, set the transfer mode to uniform acceleration, 10-50 revolutions/min, and adjust the step length to 1 revolution. Put each group of mice into the runner channel respectively, and record the time when the mice fell from the runner, which is the residence time of the mouse runner.

2.2 Mice swimming experiment method

36 mice were randomly divided into blank control group, ginseng decoction group, drug I group, drug II group according to their body weight, all were administered ig, 1 time/d, each time The medicine is 0.4 ml for one week in a row. Half an hour after the last administration, but the mice were in a mouse swimming pool with a water depth of 12 cm and a water temperature of 8 dragons. Record the time for each mouse to not rise to the surface for 10 seconds from entering the water to sinking its head into the water. Mouse swimming time.

2.3 Statistical processing

All data are expressed as mean ± standard deviation (M ± 5), using f test, P<0.05 indicates that the difference is significant. 2 results

See Table 1 for the staying time and swimming time of the mice in each group. It can be seen from Table 1 that the anti-fatigue effect of Roucong Yinyanglei Lycium barbarum Puerariae complex and Roucong Yinyanglei extract is similar, but not as good as ginseng; the three groups of drugs have anti-fatigue effects.

Table 1 Comparison of anti-fatigue effects of mice in each group

Note: Compared with the normal saline group, aP <0.05, bP <0.01; compared with the ginseng decoction group, <0.05

3. Discussion

Fatigue may be a physiological phenomenon or a precursor of a pathological process. Anti-fatigue is the use of drugs or rest to delay the appearance of these physiological phenomena. The main way to prevent and recover from fatigue is to supplement energy and remove undesirable metabolites. Wait. The most direct manifestation of the anti-fatigue ability is the improvement of sports endurance. The residence time of the runner and the swimming time can reflect the degree of fatigue resistance of the animal. The mouse swimming experiment is an anti-stress experimental method widely used in the study of the pharmacodynamics and mechanism of antidepressant, anti-fatigue, and nootropic drugs.

In this experiment, the retention time of the runner and the swimming time experiment was used to study the anti-fatigue effect of the extract of Epimedium. Ginseng decoction, meat from Epimedium. Lycium barbarum and Pueraria lobata complex, and meat from Epimedium extract all have anti-fatigue effects, but the effect of ginseng decoction is better. The anti-fatigue effect of the complex is not as good as that of ginseng.

The effective ingredients of traditional Chinese medicine are the material basis for the effect of the medicine. After the compatibility of traditional Chinese medicine, the interaction between the ingredients in the body is more complex and diverse due to the various components of the compound Chinese medicine. Studies have shown that the extract of Epiphyllum Virginia L. has a significant effect on improving exercise ability. The runner experiment and swimming experiment can cause various stress responses in the body. The extract may be anti-fatigue through the pituitary-adrenal cortex system. Role' item. Other studies have shown that Yin Yang Lei and Rou Nu Rong are rich in antioxidant system activators with certain anti-lipid peroxidation effects, which can inhibit the production of lipid peroxides in body tissues, thereby inhibiting the oxidation process in red blood cells. Has a certain anti-fatigue effect[16_1910.

There are no different dosage groups for the extract of Epiphyllum L., so whether its anti-fatigue effect is better remains to be studied. In addition, due to insufficient preparation of the experimental program when selecting the topic, no separate experimental group was set up for the two medicines of Cilantro and Epiphyllum, so the study of the extract of Epiphyte and Epiphyte in the experiment lacked a control group, it does not mean that the combination of the two medicines of Roucong Root Epidermis has a synergistic effect, an additive effect or an antagonistic effect. In addition, the medlar and Pueraria lobata root compound health product contains more traditional Chinese medicines. The medlar and Pueraria root in the compound may have an impact on the efficacy of the medicament. But our experiments show that drug I There is no significant difference in the effect of group II. Therefore, the anti-fatigue effect of the compound health care products of meat female epiphyte extract and medlar Pueraria lobata root and the interaction between the drugs need to be further studied.

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