The Effect Of The Alcoholic Extract Of Meat From The Puree On The Kidneys And Adrenal Glands Of Animals With Yang Deficiency Caused By Hormones

Pan Yurong, He Fanyin

The 148th Hospital of the People's Liberation Army,

Zhoucun District Hospital, Zibo City

Contact: joanna.jia@wecistanche.com

Traditional Chinese medicine Cistanche is the fleshy stem with scale leaves of the family plant, which has the effect of invigorating the kidney and assisting yang. We used Hydrocortisone Acetate to make a mouse "Yang Deficiency" animal model. Using the changes in the morphology and structure of the kidney and adrenal glands of the mice as indicators, we explored the protective effect of the alcohol extract of Rhizoma Rhizome on the kidney and adrenal functions.

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Material

Hydrocortisone Acetate Injection is produced by Shandong Xinhua Pharmaceutical Factory.

Alcoholic extract of Cistanche: Cistanche was purchased from a local medicinal material company.

Experimental animals: Experimental mice raised in the animal room of the PLA Hospital, weighing 22-28g.

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Experimental method

The mice were made into "Yang deficiency" animal models. Only hydrocortisone acetate was used as the experimental group, and only hydrocortisone acetate was added to the alcohol extract of meat from puree, which was the drug group and only the normal control group. Since the beginning of the experiment, the experimental group and the drug group have been injected with hydrocortisone acetonide into the buttock muscles, and the dosage is per day. The drug group was also given the alcohol extract of meat from the stomach via a gastric tube, the dose was each coin-day. The three groups of animal experiment group, drug group, and normal control group were fed with the same volume of normal saline every day, and the experiment lasted for days. There were deaths in the experimental group, deaths in the drug group, and no deaths in the normal control group. A mouse was taken from the three groups and sacrificed. The kidneys and adrenal glands were immediately dissected. The tissues were fixed with formalin solution and routine pathological examinations were performed.

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Result

In the experimental group, the adrenal cortex was atrophied, and the fascicular zone cells decreased in size. The tubular epithelial cells of the kidney are swollen, and casts can be seen in the distal convoluted tubules. In the drug group, there was no atrophy of the adrenal cortex, the thickness of the fascicular zone cells was close to normal, and there was no abnormality in the kidneys. In the normal control group, the adrenal cortex and kidney were normal. The above-mentioned experimental results show that when animals use corticosteroids for a longer period of time, the addition of alcoholic extracts of meat from the Chinese medicine for nourishing the kidney and promoting yang can prevent the adrenal cortex atrophy caused by the single use of hormones. In addition, the alcohol extract of meat from puree also has a certain protective effect on kidney function.

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