The Memory Improving And Anti- Alzheimer's Disease Effects Of Cistanche

Contact: Audrey Hu Whatsapp/hp: 0086 13880143964 Email: audrey.hu@wecistanche.com

Study on the protective effect of Total Glycosides of Cistanche on Alzheimer's disease model rats

Luo Dang

[Project A: Natural Science Foundation of Xinjiang Uygur Autonomous Region (No. 200121112)]

[Director pharmacist, associate professor, Ph.D. Research direction: pharmacology and clinical pharmacy]

Wu Xiaochuan Qi Gaohui Ji, Lu Shunzhong 1, Wang Jianhua 2, Wang Xiaomai 3

1 Department of Pharmacy, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 8300542

2 Morphology Center, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830054

3 Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830054

To study the protective effect of Total Glycosides of Cistanche on Alzheimer's disease (AD) model rats. Methods: Rats were injected with condensed Q amyloid (Ap25.35) into the dorsal part of the hippocampus using brain stereotactic technology to replicate the rat AD model. The experiment was divided into sham operation (is volume normal saline), model (iso volume normal saline), vitamin E (40 mg/kg), and Total Glycosides of Cistanche high, medium, and low dose (160.80.40 mg/kg) groups. The intragastric administration was started 4 days before copying the model, once a day for 14 consecutive days. Passive avoidance jumping experiment and electrical maze experiment were used to detect the learning and memory ability of rats; tissue homogenate and brain tissue slices of rat hippocampus were prepared to detect the activity of acetylcholinesterase (AchE) in hippocampus tissue of rats, and the rats were observed under electron microscope The intracellular C content in the hippocampal CA1 area. Results: Compared with the sham operation group, the rats in the model group had significantly longer reaction time and a significant increase in the number of errors (corpse VO.O1), hippocampal AchE activity was significantly enhanced (corpse V0.01), and intracellular C storage content in the hippocampal CA1 area increase. Compared with the model group, the reaction time of rats in the high, medium, and low dose groups of Total Glycosides of Cistanche was significantly shortened, the number of errors was significantly reduced (P<0.01), and the AchE activity in the hippocampus was significantly weakened (FV0.01 or household V0.05), The intracellular Cf+ content in the CA1 area of the hippocampus was significantly reduced. Conclusion: Total Glycosides of Cistanche can reduce the AchE activity and Ca2+ content in the hippocampus of AD model rats, maintain the normal level of acetylcholine (Ach) in the brain and improve the ability of learning and memory.

Keywords: Total Glycosides of Cistanche; Alzheimer's disease; Acetylcholinesterase; Learning and memory ability; Calciumion

Alzheimer's Disease (AD) is a degenerative brain disease that occurs in old age and is characterized by progressive cognitive impairment and memory impairment. Its pathological changes are mainly characterized by senile plaques (SP), neurofibrillary tangles (NFT), and neuron loss. The pathogenesis of air AD is very complicated, and it is mainly related to amyloid boil (A0gQ, tau protein, presenilin, oxidative stress). Factors such as stimulation and apoptosis are related to O. Among them, the A025-35 theory (Amyloidcascade hypothesis) occupies an important position in the pathogenesis of AD. The most obvious and serious change that causes AD occurs in the cholinergic system called A. Chen% can increase Acetylcholinesterase (AchE) activity reduce the content of acetylcholine (Ach) in the brain and significantly reduce the ability of learning and memory. The development and function of the cholinergic nervous system are regulated by the concentration of intracellular Ca" called Cistanches Herba. Commonly known as Dayun, a plant in the Lyndangaceae family is a well-known tonic traditional Chinese medicine that has the functions of nourishing kidney yang, nourishing essence and blood, moisturizing the intestines and laxative, anti-aging, etc.' package (Glycosides of Cistanche Herba, Total Glycosides of Cistanche) from the north of Xinjiang Extracted from Xinjiang cistanche tubulosa, the main ingredient is Cistanche Total Glycosides, which has the effects of protecting myocardial ischemia, anti-radiation, anti-oxidation, scavenging free radicals in vitro, enhancing human immune function, and delaying aging. “Ginseng” has been listed in the 2010 edition of the Chinese Pharmacopoeia. The previous work of this research group has confirmed that Total Glycosides of Cistanche have a significant protective effect on neuro cytotoxicity caused by A wound ball. At the same time, it has been found that Total Glycosides of Cistanche can improve the learning and memory ability of AD mice caused by Pi 2 ball, and improve certain pathological changes in the brain tissue. Therefore, based on this research, the author further studied the effect of Total Glycosides of Cistanche on the AD caused by A% Yasuo. The learning and memory ability of mice and the influence of AchE activity in the brain and the content of Ca,+ provide a basis for the treatment of AD and promote the development and utilization of Cistanche.

1 material

1.1 Instrument

CKX41 type electron microscope (Olympus, Japan); LSY type electric heating constant temperature water bath (Beijing Medical Equipment Factory); Electronic balance (Beijing Sartorius Balance Co., Ltd.); LYJ-HI type centrifugal precipitation machine (Jiangsu Xiangshui Medical Equipment Factory); 721 type spectrophotometer (Shanghai No. 3 Analytical Instrument Factory); WH-851 type vortex mixer (Shanghai Huanqiu Physical Chemical Instrument Factory); TN-type tray-type torsion balance (Shanghai No. 2 Tianping Instrument Factory); Jiangwan I Type C Brain stereotaxic device (Second Military Medical University); platform jumping device (Functional Experimental Center of Xinjiang Medical University); third-arm Y-type maze (Zhangjiagang Sanxing Teaching Machinery Factory).

1.2 Medicinal materials

Cistanche extracts (from Beijiang, Xinjiang) were identified as genuine by Chief Pharmacist Yang Jianhua of the Third Affiliated Hospital of Xinjiang Medical University.

1.3 Drugs and reagents

Total Glycosides of Cistanche (made by the Department of Natural Medicines/Biopharmaceuticals, School of Pharmacy, Xinjiang Medical University, the main ingredient is Phenylethanoild Glycosides, the purity determined by UV method is >50%); A Yuqiu (American Sigma Company, batch number: 115k4778); Vitamin E (Vit E), Shanghai Sanwei Pharmaceutical Co., Ltd., batch number: 20070605); AchE test kit (Nanjing Jiancheng Institute of Bioengineering).

1.4 Animals

60 adult healthy SD rats, 8, weighing (220±270) g, were provided by the Experimental Animal Center of Xinjiang Medical University (animal production certificate number: Yidongzi No. 16-003).

2 Method

2.1 Grouping and administration

The experiment is divided into 6 groups, namely sham operation (iso volume normal saline), model (iso volume normal saline), Vit E (50 mg/kg), and Total Glycosides of Cistanche high, medium, and low doses (160, 80, 40 mg) /kg) group, ig administration was started 4 days before copying the model, once a day for 14 consecutive days.

2.2 Copy of the model

Dissolve 1 mg of A wound ball in 200 J1 sterilized physiological saline, seal it and place it in a 37 T cell incubator, and form a condensed state after 7 days. Rats are anesthetized with chloral hydrate (400 mg/kg) and routinely prepared and disinfected. The skin is cut in the middle of the top of the skull, fixed with a stereotaxic instrument, and the head is flattened. Determined according to the stereotaxic map of the rat The backside of the right hippocampus is 3.8 mm, 2.5 mm next to the midline, and 3.0 mm below the surface of the skull, which is the dorsal part of the right hippocampus. Withdraw the cerebrospinal fluid, slowly inject within 5 minutes with a 10 microinjector, and retain the needle for 10 minutes. After penicillin sodium 50,000 u, once a day for 3 days.

2.3 Detection of indicators

2.3.1 Rats’ passive avoidance platform jumping experiment 14 days after the operation, but the rats into the platform reaction box, adapt to the environment on the copper grid for 3 minutes, turn on the power (90V alternating current), the rats received an electric shock, the normal reaction is Jump on the platform to avoid noxious stimuli.

Total Glycosides of Cistanche significantly improve the learning and memory ability of AD-induced rats, shortens the response time to electrical stimulation, reduces the number of errors, and reduces the activity of AchE and the accumulation of Ca" in the hippocampal CA1 area. In summary, Total Glycosides of Cistanche has a protective effect on the learning and memory impairment of AD model rats caused by A", and thus plays a role in the treatment of memory and cognitive impairment. One of its mechanisms may be to maintain the normal level of Ach in the brain and reduce the content of Ca. It is related to the learning and memory ability of AD model rats. Our group intends to further study from molecular biology whether Total Glycosides of Cistanche can inhibit the hyperphosphorylation of tau-protein deposition by down-regulating the activity of glycogen synthase kinase 30, thereby Inhibit the deposition of A and e, exerting its neuron protective effect.

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